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Human Cell

, Volume 30, Issue 2, pp 117–123 | Cite as

Infection of defective human T-lymphotropic virus type 1

  • Yuuki Hashikura
  • Kazumi Umeki
  • Kunihiko Umekita
  • Hajime Nomura
  • Akiteru Yamada
  • Ikuo Yamamoto
  • Hiroo Hasegawa
  • Katsunori Yanagihara
  • Akihiko OkayamaEmail author
Research Article

Abstract

In a previous study, we reported that an identical defective provirus had integrated into multiple sites of the genome of a representative human T-lymphotropic virus type 1 (HTLV-1) cell line, MT-2. A possible explanation for this may be the repeated infection of this defective provirus to a cell. Therefore, we attempted to determine whether a defective provirus could transmit during the co-culture of HTLV-1 uninfected human T-cell line, Jurkat, with MT-2 cells treated with mitomycin C. As a result, we established not only a cell line with the integration of one complete provirus, but also a cell line with the integration of one defective provirus. The rearrangement of the T-cell receptor -γ gene of these cell lines showed them to be derived from Jurkat cells. Both HTLV-1 Tax/Rex and HBZ RNA were detected in the cell line, which harbors a complete provirus. On the other hand, HBZ RNA and transcriptional product specific for the defective provirus were detected in the cell line, which harbors a defective HTLV-1 provirus only. These results suggested that a defective HTLV-1 provirus with large depletion of internal sequence could transmit to other cells. Moreover, the defective provirus can be transcriptionally active. This suggested the possibility that the defective HTLV-1 provirus found in the lymphocytes of HTLV-1 carriers and patients with adult T-cell leukemia may transmit to other T-cells in vivo. The results also suggested that defective provirus in HTLV-1 carriers could be functional and may play a role in leukemogenesis.

Keywords

HTLV-1 Defective virus MT-2 cell line 

Notes

Acknowledgements

The authors would like to thank Ms. Y. Kaseda and Ms. K. Takatsuka (Miyazaki University) for their technical support and assistance.

Compliance with ethical standards

Conflict of interest

We have no disclosure.

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Copyright information

© Japan Human Cell Society and Springer Japan 2017

Authors and Affiliations

  • Yuuki Hashikura
    • 1
  • Kazumi Umeki
    • 1
  • Kunihiko Umekita
    • 1
  • Hajime Nomura
    • 1
  • Akiteru Yamada
    • 1
  • Ikuo Yamamoto
    • 1
  • Hiroo Hasegawa
    • 2
  • Katsunori Yanagihara
    • 2
  • Akihiko Okayama
    • 1
    Email author
  1. 1.Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of MedicineUniversity of MiyazakiKiyotakeJapan
  2. 2.Department of Laboratory MedicineNagasaki University Graduate School of Biomedical SciencesNagasakiJapan

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