Human Cell

, Volume 30, Issue 2, pp 140–148 | Cite as

Establishment and characterization of a novel uterine carcinosarcoma cell line, TU-ECS-1, with mutations of TP53 and KRAS

  • Yohei Chiba
  • Seiya Sato
  • Hiroaki ItamochiEmail author
  • Yasuko Suga
  • Tomoyuki Fukagawa
  • Nao Oumi
  • Tetsuro Oishi
  • Tasuku Harada
  • Tamotsu Sugai
  • Toru Sugiyama
Cell Line


A new human uterine carcinosarcoma (UCS) cell line, TU-ECS-1, was established and characterized. The morphological appearance of the cultured cells was an insular of epithelial-like cells arranged in the form of a jigsaw puzzle and mesenchymal-like cells with a spindle-shaped or fibroblast-like morphology. A relatively high proliferation rate was observed with a doubling time of 18.2 h. The chromosome number ranged from 44 to 49 and had an extra chromosome 12 (trisomy 12). The respective half-maximal inhibitory concentrations of cisplatin, paclitaxel, and doxorubicin were 2.9 µM, 154 nM, and 219 ng/mL, respectively. Mutational analysis revealed that TU-ECS-1 cells have mutations of TP53 in exons 4, 6, and 8 and of KRAS at codon 12 (G12D) in exon 2, which is a mutation hot spot on this gene. Western blot analysis showed that p53 protein was overexpressed in TU-ECS-1 cells. Immunostaining of the cultured cells and in vivo tumors showed that the TU-ECS-1 cells and xenografts were positive for epithelial marker cytokeratin AE1/3 and mesenchymal marker vimentin. These results suggested that TU-ECS-1 cells might have both epithelial and mesenchymal characteristics. This cell line may be useful to study the carcinogenesis of UCS and contribute to the development of novel treatment strategies.


Uterine carcinosarcoma Establishment TP53 KRAS Molecular-targeted therapy 



We thank Mr. Noriyuki Yamada of the Department of Pathology at Iwate Medical University School of Medicine for assisting with immunohistochemical analysis. The present study was supported by the Project of Human Resource Development for Cancer from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Leading Center for the Development and Research of Cancer Medicine from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Japan Human Cell Society and Springer Japan 2016

Authors and Affiliations

  • Yohei Chiba
    • 1
  • Seiya Sato
    • 1
  • Hiroaki Itamochi
    • 1
    Email author
  • Yasuko Suga
    • 1
  • Tomoyuki Fukagawa
    • 1
    • 2
  • Nao Oumi
    • 3
  • Tetsuro Oishi
    • 3
  • Tasuku Harada
    • 3
  • Tamotsu Sugai
    • 2
  • Toru Sugiyama
    • 1
  1. 1.Department of Obstetrics and GynecologyIwate Medical University School of MedicineMoriokaJapan
  2. 2.Department of PathologyIwate Medical University School of MedicineMoriokaJapan
  3. 3.Department of Obstetrics and GynecologyTottori University School of MedicineYonagoJapan

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