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Human Cell

, Volume 29, Issue 2, pp 58–66 | Cite as

Identification of a highly immunogenic mouse breast cancer sub cell line, 4T1-S

  • Hirotake Abe
  • Haruka Wada
  • Muhammad Baghdadi
  • Sayaka Nakanishi
  • Yuu Usui
  • Takahiro Tsuchikawa
  • Toshiaki Shichinohe
  • Satoshi Hirano
  • Ken-ichiro SeinoEmail author
Rapid Communication

Abstract

Cancer vaccines serve as a promising clinical immunotherapeutic strategy that help to trigger an effective and specific antitumor immune response compared to conventional therapies. However, poor immunogenicity of tumor cells remains a major obstacle for clinical application, and developing new methods to modify the immunogenicity of tumor cells may help to improve the clinical outcome of cancer vaccines. 4T1 mouse breast cancer cell line has been known as poorly immunogenic and highly metastatic cell line. Using this model, we identified a sub cell line of 4T1—designated as 4T1-Sapporo (4T1-S)—which shows immunogenic properties when used as a vaccine against the same line. In 4T1-S-vaccinated mice, subcutaneous injection of 4T1-S resulted in an antitumor inflammatory response represented by significant enlargement of draining lymph nodes, accompanied with increased frequencies of activated CD8 T cells and a subpopulation of myeloid cells. Additionally, 4T1-S vaccine was ineffective to induce tumor rejection in nude mice, which importantly indicate that 4T1-S vaccine rely on T cell response to induce tumor rejection. Further analysis to identify mechanisms that control tumor immunogenicity in this model may help to develop new methods for improving the efficacies of clinical cancer vaccines.

Keywords

Breast cancer 4T1 Immunogenicity Transformation 

Abbreviations

4T1-A

4T1-ATCC

4T1-S

4T1-Sapporo

DLN

Draining lymph node

RLN

Remote lymph node

TIL

Tumor infiltrating lymphocytes

Notes

Acknowledgments

This study was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (KS). This work was also supported in part by the Suhara Foundation (KS).

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.

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Copyright information

© Japan Human Cell Society and Springer Japan 2016

Authors and Affiliations

  • Hirotake Abe
    • 1
    • 2
  • Haruka Wada
    • 1
  • Muhammad Baghdadi
    • 1
  • Sayaka Nakanishi
    • 1
  • Yuu Usui
    • 1
  • Takahiro Tsuchikawa
    • 2
  • Toshiaki Shichinohe
    • 2
  • Satoshi Hirano
    • 2
  • Ken-ichiro Seino
    • 1
    Email author
  1. 1.Division of Immunobiology, Institute for Genetic MedicineHokkaido UniversitySapporoJapan
  2. 2.Department of Gastroenterological Surgery IIHokkaido University Graduate School of MedicineSapporoJapan

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