Human Cell

, Volume 26, Issue 3, pp 114–120

Effects of extracellular calcium on viability and osteogenic differentiation of bone marrow stromal cells in vitro

  • Shaowen Cheng
  • Wei Wang
  • Zhongqin Lin
  • Ping Zhou
  • Xiaolei Zhang
  • Wei Zhang
  • Qingyu Chen
  • Dongquan Kou
  • Xiaozhou Ying
  • Yue Shen
  • Xiaojie Cheng
  • Ziming Yu
  • Lei Peng
  • Chuanzhu Lu
Research Article

DOI: 10.1007/s13577-012-0041-8

Cite this article as:
Cheng, S., Wang, W., Lin, Z. et al. Human Cell (2013) 26: 114. doi:10.1007/s13577-012-0041-8

Abstract

Bone marrow stromal cells (BMSCs) have been extensively used for tissue engineering. However, the effect of Ca2+ on the viability and osteogenic differentiation of BMSCs has yet to be evaluated. To determine the dose-dependent effect of Ca2+ on viability and osteogenesis of BMSCs in vitro, BMSCs were cultured in calcium-free DMEM medium supplemented with various concentrations of Ca2+ (0, 1, 2, 3, 4, and 5 mM) from calcium citrate. Cell viability was analyzed by MTT assay and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) assay, Von Kossa staining, and real-time PCR. Ca2+ stimulated BMSCs viability in a dose-dependent manner. At slightly higher concentrations (4 and 5 mM) in the culture, Ca2+ significantly inhibited the activity of ALP on days 7 and 14 (P < 0.01 or P < 0.05), significantly suppressed collagen synthesis (P < 0.01 or P < 0.05), and significantly elevated calcium deposition (P < 0.01) and mRNA levels of osteocalcin (P < 0.01 or P < 0.05) and osteopontin (P < 0.01 or P < 0.05). Therefore, elevated concentrations of extracellular calcium may promote cell viability and late-stage osteogenic differentiation, but may suppress early-stage osteogenic differentiation in BMSCs.

Keywords

Mesenchymal stem cell Osteogenesis Calcium Viability Differentiation 

Copyright information

© Japan Human Cell Society and Springer 2013

Authors and Affiliations

  • Shaowen Cheng
    • 2
  • Wei Wang
    • 1
  • Zhongqin Lin
    • 4
  • Ping Zhou
    • 3
  • Xiaolei Zhang
    • 1
  • Wei Zhang
    • 1
  • Qingyu Chen
    • 1
  • Dongquan Kou
    • 1
  • Xiaozhou Ying
    • 1
  • Yue Shen
    • 1
  • Xiaojie Cheng
    • 1
  • Ziming Yu
    • 2
  • Lei Peng
    • 2
  • Chuanzhu Lu
    • 2
  1. 1.Department of Orthopaedic SurgeryThe Second Affiliated Hospital of Wenzhou Medical CollegeWenzhouChina
  2. 2.Trauma Center of the Affiliated Hospital of Hainan Medical CollegeHaikouChina
  3. 3.Department of OncologyThe First Affiliated Hospital of Zhejiang UniversityHangzhouChina
  4. 4.Department of Orthopaedic SurgeryThe Hospital of Integrated Traditional Chinese and Western Medicine of WenzhouWenzhouChina

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