Human Cell

, Volume 26, Issue 1, pp 19–27

Derivation of multipotent nestin+/CD271/STRO-1 mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions

  • Rongrong Wu
  • Bin Gu
  • Xiaoli Zhao
  • Zhou Tan
  • Liangbiao Chen
  • Jiang Zhu
  • Ming Zhang
Research Article

Abstract

The successful establishment of stem cell-based therapies requires multipotent, immunocompatible stem cells, highly efficient strategies for direct differentiation, and most importantly, optimal culture conditions for large-scale expansion of such cell populations. Other than adult tissues, human embryonic stem cells (hESCs) represent another infinitely expansible source for mesenchymal stem cell (MSC) derivation. Here, we reproducibly derived a population of Nestin+/CD271/STRO-1 mesenchymal-like precursors from hESCs (hESC-MPs) in chemically defined conditions, without requiring any serum or serum replacement of animal origin, based on a Y-27632-assisted monolayer culture system. These cells showed slim fibroblastic morphology, and satisfied the criteria of MSCs including self-renewal, the expression of multiple MSC-specific markers and the ability to differentiate into osteoblasts, adipocytes and chondrocytes. Compared with previously reported hESC-derived MSCs, our hESC-MPs were more multipotent, and could differentiate into representative derivatives of all three embryonic germ layers including mature smooth muscle cells, cardiomyocytes, functional hepatocytes and neural cells expressing various neurotransmitter phenotypes, making them an attractive cell source for regenerative medicine.

Keywords

Chemically defined conditions Human embryonic stem cells Mesenchymal-like precursors Y-27632 

Supplementary material

13577_2011_22_MOESM1_ESM.doc (114 kb)
Supplementary material 1 (DOC 114 kb)

References

  1. 1.
    Banas A, Teratani T, Yamamoto Y, et al. Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes. Hepatology. 2007;46:219–28.PubMedCrossRefGoogle Scholar
  2. 2.
    Chamberlain G, Fox J, Ashton B, Middleton J. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells. 2007;25:2739–49.PubMedCrossRefGoogle Scholar
  3. 3.
    Lian Q, Lye E, Suan Yeo K, et al. Derivation of clinically compliant MSCs from CD105+, CD24− differentiated human ESCs. Stem Cells. 2007;25:425–36.PubMedCrossRefGoogle Scholar
  4. 4.
    Liu YP, Seckin H, Izci Y, Du ZW, Yan YP, Baskaya MK. Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab. 2009;29:780–91.PubMedCrossRefGoogle Scholar
  5. 5.
    Barberi T, Willis LM, Socci ND, Studer L. Derivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med. 2005;2:e161.PubMedCrossRefGoogle Scholar
  6. 6.
    Olivier EN, Rybicki AC, Bouhassira EE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells. 2006;24:1914–22.PubMedCrossRefGoogle Scholar
  7. 7.
    Barberi T, Bradbury M, Dincer Z, Panagiotakos G, Socci ND, Studer L. Derivation of engraftable skeletal myoblasts from human embryonic stem cells. Nat Med. 2007;13:642–8.PubMedCrossRefGoogle Scholar
  8. 8.
    Mahmood A, Harkness L, Schroder HD, Abdallah BM, Kassem M. Enhanced differentiation of human embryonic stem cells to mesenchymal progenitors by inhibition of TGF-beta/activin/nodal signaling using SB-431542. J Bone Miner Res. 2010;25:1216–33.PubMedCrossRefGoogle Scholar
  9. 9.
    Xu C, Jiang J, Sottile V, McWhir J, Lebkowski J, Carpenter MK. Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth. Stem Cells. 2004;22:972–80.PubMedCrossRefGoogle Scholar
  10. 10.
    Hwang NS, Varghese S, Lee HJ, et al. In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci USA. 2008;105:20641–6.PubMedCrossRefGoogle Scholar
  11. 11.
    Brown SE, Tong W, Krebsbach PH. The derivation of mesenchymal stem cells from human embryonic stem cells. Cells Tissues Organs. 2009;189:256–60.PubMedCrossRefGoogle Scholar
  12. 12.
    Lee EJ, Lee HN, Kang HJ, et al. Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells. Tissue Eng Part A. 2009;16:705–15.CrossRefGoogle Scholar
  13. 13.
    Gerrard L, Rodgers L, Cui W. Differentiation of human embryonic stem cells to neural lineages in adherent culture by blocking bone morphogenetic protein signaling. Stem Cells. 2005;23:1234–41.PubMedCrossRefGoogle Scholar
  14. 14.
    Erceg S, Lainez S, Ronaghi M, et al. Differentiation of human embryonic stem cells to regional specific neural precursors in chemically defined medium conditions. PLoS One. 2008;3:e2122.PubMedCrossRefGoogle Scholar
  15. 15.
    Amit M, Carpenter MK, Inokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol. 2000;227:271–8.PubMedCrossRefGoogle Scholar
  16. 16.
    Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282:1145–7.PubMedCrossRefGoogle Scholar
  17. 17.
    Watanabe K, Ueno M, Kamiya D, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007;25:681–6.PubMedCrossRefGoogle Scholar
  18. 18.
    Mannello F, Tonti GA. Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells. 2007;25:1603–9.PubMedCrossRefGoogle Scholar
  19. 19.
    Heiskanen A, Satomaa T, Tiitinen S, et al. N-glycolylneuraminic acid xenoantigen contamination of human embryonic and mesenchymal stem cells is substantially reversible. Stem Cells. 2007;25:197–202.PubMedCrossRefGoogle Scholar
  20. 20.
    Sakamoto N, Tsuji K, Muul LM, et al. Bovine apolipoprotein B-100 is a dominant immunogen in therapeutic cell populations cultured in fetal calf serum in mice and humans. Blood. 2007;110:501–8.PubMedCrossRefGoogle Scholar
  21. 21.
    Hisamatsu-Sakamoto M, Sakamoto N, Rosenberg AS. Embryonic stem cells cultured in serum-free medium acquire bovine apolipoprotein B-100 from feeder cell layers and serum replacement medium. Stem Cells. 2008;26:72–8.PubMedCrossRefGoogle Scholar
  22. 22.
    Martin MJ, Muotri A, Gage F, Varki A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med. 2005;11:228–32.PubMedCrossRefGoogle Scholar
  23. 23.
    Cowan CA, Klimanskaya I, McMahon J, et al. Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med. 2004;350:1353–6.PubMedCrossRefGoogle Scholar
  24. 24.
    Wu R, Xu C, Jin F, et al. Derivation, characterization and differentiation of a new human embryonic stem cell line from a Chinese hatched blastocyst assisted by a non-contact laser system. Hum Cell. 2010;23:89–102.PubMedCrossRefGoogle Scholar
  25. 25.
    Yamada T, Yoshikawa M, Kanda S, et al. In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Stem Cells. 2002;20:146–54.PubMedCrossRefGoogle Scholar
  26. 26.
    Rodriguez LV, Alfonso Z, Zhang R, Leung J, Wu B, Ignarro LJ. Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells. Proc Natl Acad Sci USA. 2006;103:12167–72.PubMedCrossRefGoogle Scholar
  27. 27.
    Huang H, Zhao X, Chen L, et al. Differentiation of human embryonic stem cells into smooth muscle cells in adherent monolayer culture. Biochem Biophys Res Commun. 2006;351:321–7.PubMedCrossRefGoogle Scholar
  28. 28.
    Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med. 2004;8:301–16.PubMedCrossRefGoogle Scholar
  29. 29.
    Quirici N, Soligo D, Bossolasco P, Servida F, Lumini C, Deliliers GL. Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp Hematol. 2002;30:783–91.PubMedCrossRefGoogle Scholar
  30. 30.
    Galan-Caridad JM, Harel S, Arenzana TL, et al. Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell. 2007;129:345–57.PubMedCrossRefGoogle Scholar
  31. 31.
    Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol. 2003;21:183–6.PubMedCrossRefGoogle Scholar
  32. 32.
    Hotta R, Pepdjonovic L, Anderson RB, et al. Small-molecule induction of neural crest-like cells derived from human neural progenitors. Stem Cells. 2009;27:2896–905.PubMedGoogle Scholar
  33. 33.
    Yao S, Chen S, Clark J, et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci USA. 2006;103:6907–12.PubMedCrossRefGoogle Scholar
  34. 34.
    Sze SK, de Kleijn DP, Lai RC, et al. Elucidating the secretion proteome of human embryonic stem cell-derived mesenchymal stem cells. Mol Cell Proteomics. 2007;6:1680–9.PubMedCrossRefGoogle Scholar
  35. 35.
    Pochampally RR, Smith JR, Ylostalo J, Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood. 2004;103:1647–52.PubMedCrossRefGoogle Scholar

Copyright information

© Japan Human Cell Society and Springer 2011

Authors and Affiliations

  • Rongrong Wu
    • 1
    • 2
  • Bin Gu
    • 1
  • Xiaoli Zhao
    • 1
  • Zhou Tan
    • 1
  • Liangbiao Chen
    • 3
  • Jiang Zhu
    • 4
  • Ming Zhang
    • 1
  1. 1.College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, The Second Affiliated Hospital, College of MedicineZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Institute of Genetics and Developmental BiologyChinese Academy of ScienceBeijingPeople’s Republic of China
  4. 4.Shanghai Institute of Hematology, Rui-Jin Hospital, College of MedicineShanghai Jiao-Tong UniversityShanghaiPeople’s Republic of China

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