Human Cell

, Volume 26, Issue 1, pp 19–27

Derivation of multipotent nestin+/CD271/STRO-1 mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions

  • Rongrong Wu
  • Bin Gu
  • Xiaoli Zhao
  • Zhou Tan
  • Liangbiao Chen
  • Jiang Zhu
  • Ming Zhang
Research Article


The successful establishment of stem cell-based therapies requires multipotent, immunocompatible stem cells, highly efficient strategies for direct differentiation, and most importantly, optimal culture conditions for large-scale expansion of such cell populations. Other than adult tissues, human embryonic stem cells (hESCs) represent another infinitely expansible source for mesenchymal stem cell (MSC) derivation. Here, we reproducibly derived a population of Nestin+/CD271/STRO-1 mesenchymal-like precursors from hESCs (hESC-MPs) in chemically defined conditions, without requiring any serum or serum replacement of animal origin, based on a Y-27632-assisted monolayer culture system. These cells showed slim fibroblastic morphology, and satisfied the criteria of MSCs including self-renewal, the expression of multiple MSC-specific markers and the ability to differentiate into osteoblasts, adipocytes and chondrocytes. Compared with previously reported hESC-derived MSCs, our hESC-MPs were more multipotent, and could differentiate into representative derivatives of all three embryonic germ layers including mature smooth muscle cells, cardiomyocytes, functional hepatocytes and neural cells expressing various neurotransmitter phenotypes, making them an attractive cell source for regenerative medicine.


Chemically defined conditions Human embryonic stem cells Mesenchymal-like precursors Y-27632 

Supplementary material

13577_2011_22_MOESM1_ESM.doc (114 kb)
Supplementary material 1 (DOC 114 kb)


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Copyright information

© Japan Human Cell Society and Springer 2011

Authors and Affiliations

  • Rongrong Wu
    • 1
    • 2
  • Bin Gu
    • 1
  • Xiaoli Zhao
    • 1
  • Zhou Tan
    • 1
  • Liangbiao Chen
    • 3
  • Jiang Zhu
    • 4
  • Ming Zhang
    • 1
  1. 1.College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, The Second Affiliated Hospital, College of MedicineZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Institute of Genetics and Developmental BiologyChinese Academy of ScienceBeijingPeople’s Republic of China
  4. 4.Shanghai Institute of Hematology, Rui-Jin Hospital, College of MedicineShanghai Jiao-Tong UniversityShanghaiPeople’s Republic of China

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