Cloning and expression analysis of the StCUL1 gene in potato
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The Cullin-RING E3 ubiquitin ligase (CRL) complex is the most common E3 ligase, and the SCF complex (CRL1) has the most diverse functions. Cullin1(CUL1) is a scaffolding protein for assembly of the complex. SCF has been shown to participate in the non-biological stress response pathways. In this study, a classic CUL1 protein was identified in Solanum tuberosum, StCUL1. A full-length cDNA of the StCUL1 gene was obtained from ED13 (a potato variety) by Ralstonia solanacearum inoculation using the RACE method. Sequence analysis indicated that the gene comprised 2662 bp, with an open reading frame of 2229 bp encoding 743 amino acids. The expression levels of the StCUL1 gene in potato treated with R. solanacearum and exogenous hormones (such as salicylic acid, jasmonic acid methyl ester and abscisic acid) at different time points were determined by real-time PCR. The results indicated that StCUL1 was induced not only by pathogenic bacteria, but also by exogenous hormones, with sustained high expression. However, there were some differences in the modes of expression. Tissue localization analysis indicated that its expression was tissue specific, and it was mainly in the phloem of the vascular system of stems and leaves.
KeywordsPotato Ralstonia solanacearum Cullin1 StCUL1 Gene expression Tissue localization
Cullin-RING E3 ubiquitin ligase
Homology to E6-AP Carboxyl Terminus
Cell division control protein 53
Suppressor of kinetochore protein 1
S-phase kinase-associated protein 1
MORE AXILLARY BRANCHES 2
We would like to thank the institutes of Vegetables and Flowers and Plant Protection of the Chinese Academy of Agricultural Sciences for potato materials. This work was supported by the project of National Natural Science Foundation of China (31771858).
Compliance with ethical standards
Conflict of interest
No conflict of interest was declared.
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