Species-specific genes account for the differences in floral transition between continuous-flowering and once-flowering roses
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Floral transitions in continuous-flowering Rosa chinensis ‘Old Blush’ and once-flowering R. odorata var. gigantea were analyzed using comparative transcriptome. In total, 3208 and 5825 species-specific unigenes were identified in the transcriptome profiles of ‘Old Blush’ and R. odorata var. gigantea, respectively. Furthermore, these species-specific unigenes were annotated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and the Mercator web tool was used to mine vital metabolic processes. The contents of soluble sugar and starch in ‘Old Blush’ were higher than those in R. odorata var. gigantea at each stage during the floral transition process. The soluble sugar content was negatively correlated with the starch content in ‘Old Blush’. Therefore, we concluded that the vegetative-to-reproductive phase transition of ‘Old Blush’ was largely controlled by carbon metabolism, especially trehalose. In contrast, the floral transition of R. odorata var. gigantea was potentially mediated by low temperatures (vernalization) and the hexose-mediated miR156 pathway.
KeywordsRoses Floral transition Trehalose metabolism Hexose metabolism Vernalization Transcriptome
Kyoto Encyclopedia of Genes and Genomes
Granule-bound starch synthase 1
Vegetative meristem of secondary axillary bud in Rosa odorata var. gigantea
This research was supported by the Fundamental Research Funds for the Central Universities and National Natural Science Foundation of China (31600565). We thank Yuyong Yang (Kunming Yang Chinese Rose Gardening Co., Ltd.) and Haiquan Huang (Southwest Forestry University) for help with sample collection. We also thank the OmicShare forum, a free online platform for data analysis (www.omicshare.com/tools).
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Conflict of interest
The authors declare that there is no conflict of interest.
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