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Ectopic expression of a grape vine vacuolar NHX antiporter enhances transgenic potato plant tolerance to salinity

  • Safa Charfeddine
  • Mariam Charfeddine
  • Mohsen Hanana
  • Radhia Gargouri-Bouzid
Original Article
  • 5 Downloads

Abstract

Salinity is a crucial environmental constraint that reduces plant productivity. However, plants activate different signaling pathways to overcome the abiotic stress. The NHX (Na+/H+ exchanger) antiporter corresponds to one of the antiporters involved in response to salinity. They are known to be responsible for the vacuole compartmentation of toxic Na+. In this report, a grapevine vacuolar antiporter (VvNHX1) cDNA was introduced into potato, response of transgenic plants to salinity was evaluated under in vitro and greenhouse culture conditions. The transgenic plants showed higher growth rate than wild type (WT) after the salinity treatment suggesting an improved tolerance both in vitro and under greenhouse culture conditions. In addition, a lower oxidative stress level was observed while a higher relative water and soluble sugar content were measured in transgenic plants compared to WT plants. Furthermore, in contrast to WT plants, the transgenic plants displayed an increase of leaf ion (K+, Mg2+) content and a decline in Na+ accumulation. The increase in the antioxidant enzyme activities in transgenic plants suggests that they can overcome oxidative stress resulting from salt treatment. The measurement of the tuber yield and the weight loss of plants sprinkled with 100 mM NaCl in the greenhouse showed a low negative effect on transgenic plants (12.5 and 40%) in comparison to WT (80%).

Keywords

Salinity Vacuolar Na+/H+ antiporter VvNHX1 potato 

Abbreviations

ROS

Reactive oxygen species

NHX

Na+/H+ antiporter

SOD

Superoxide dismutase

CAT

Catalase

GPX

Gluthatione peroxidase

MDA

Malondialdehyde

TCA

Trichloroacetic acid

TBA

Thiobarbituric acid

RWC

Relative water content

ANOVA

Analysis of variance

WT

Wild type

Notes

Acknowledgements

This work was financed by the Tunisian Ministry of High Education and Scientific Research. The authors thank to Anne-Lise Haenni from the Institute Jacques Monod (France) and Mrs Najoua Neifar english teacher from the University of Sfax-Tunisia and Miss cyrine Bouzid from ODDO BHF for improving the English throughout the manuscript.

Authors’ contributions

SC and MC carried out the experimental work. The insertion of the VvNHX1 gene into the pCAMBIA99.1 vector was generated by MH. RGB participated in the data analysis and contributed in writing and revising the manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  • Safa Charfeddine
    • 1
  • Mariam Charfeddine
    • 1
  • Mohsen Hanana
    • 2
  • Radhia Gargouri-Bouzid
    • 1
  1. 1.Laboratory of Plant improvement and Valorization of Agro-ressources, National School of Engineering of Sfax (ENIS)Sfax UniversitySfaxTunisia
  2. 2.Laboratory of Extremophilic PlantBiotechnology Center of Borj CedriaBorj CedriaTunisia

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