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Differences in fennel seed responses to drought stress at the seed formation stage in sensitive and tolerant genotypes

  • Ehsaneh Khodadadi
  • Akiko Hashiguchi
  • Barat Ali Fakheri
  • Saeed Aharizad
  • Abbasali Emamjomeh
  • Majid Norouzi
  • Setsuko Komatsu
Original Article
  • 13 Downloads

Abstract

To understand the effects of drought on fennel seed production and determine the underlying molecular processes, various fennel genotypes were exposed to drought stress. The yield and quality, including aromatic oil content, of fennel seeds were reduced by drought during seed development. To explore drought-induced biological processes in fennel, a label-free/gel-free proteomic analysis was performed. In Gaziantep and Tatmaj cultivars, which are sensitive and tolerant fennel genotypes, respectively, 106 and 92 drought-responsive proteins were identified. Comparison of protein-functional profiles indicated that proteins classified in stress, cell, and protein synthesis/degradation categories consisted important responsive mechanisms against drought stress. Pathway analysis visualized that the tricarboxylic acid cycle is important for both cultivars. In Tatmaj, moderate activation of proteins related to oxidative pentose phosphate pathway was detected along with an increase in photosynthesis-related proteins. Furthermore, cluster analysis of drought-responsive proteins using protein abundance at milky, dough, and mature stages identified protein homeostasis as a mechanism of drought tolerance in fennel. These results suggest that coordinated energy consumption and supply might be a drought-tolerance mechanism in fennel plants.

Keywords

Fennel Seed Drought Drought-sensitive genotypes Drought-tolerant genotypes Proteomics 

Abbreviations

TCA

Tricarboxylic acid

OPP

Oxidative pentose phosphate

ROS

Reactive oxygen species

MS

Mass spectrometry

LC

Liquid chromatography

Notes

Acknowledgements

The authors thank Dr. X. Wang at the University of Tsukuba for experimental support and data analyses.

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interest.

Supplementary material

13562_2018_461_MOESM1_ESM.docx (59 kb)
Supplementary material 1 (DOCX 58 kb)
13562_2018_461_MOESM2_ESM.docx (49 kb)
Supplementary material 2 (DOCX 49 kb)
13562_2018_461_MOESM3_ESM.docx (62 kb)
Supplementary material 3 (DOCX 61 kb)
13562_2018_461_MOESM4_ESM.docx (47 kb)
Supplementary material 4 (DOCX 46 kb)

References

  1. Ahuja I, deVos RCH, Bones AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci 15:664–674CrossRefPubMedGoogle Scholar
  2. Aprotosoaie AC, Şpac A, Hăncianu M, Miron A, Tănăsescu VF, Dorneanu V, Stănescu U (2010) The chemical profile of essential oils obtained from fennel fruits (Foeniculum vulgare MILL.). Farmacia 58:46–53Google Scholar
  3. Askari E, Ehsanzadeh P (2015) Drought stress mitigation by foliar application of salicylic acid and their interactive effects on physiological characteristics of fennel (Foeniculum vulgare Mill.) genotypes. Acta Physiol Plant 37:4CrossRefGoogle Scholar
  4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  5. Caruso G, Cavaliere C, Guarino C, Gubbiotti R, Foglia P, Laganà A (2008) Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Anal Bioanal Chem 391:381–390CrossRefPubMedGoogle Scholar
  6. Choi E, Hwang J (2004) Antiinflammatory analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia 75:57–565Google Scholar
  7. Diaaz-Maroto MC, Pea rez-Coello MS, Esteban J, Sanz J (2006) Comparison of the volatile composition of wild fennel samples (Foeniculum vulgare Mill.) from Central Spain. J Agric Food Chem 54:814–6818CrossRefGoogle Scholar
  8. Ehsanipour A, Razmjoo J, Zeinali H (2012) Effect of nitrogen rates on yield and quality of fennel (Foeniculum vulgare Mill.) accessions. Ind Crops Prod 35:121–125CrossRefGoogle Scholar
  9. Farooq M, Wahid A, Komayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212CrossRefGoogle Scholar
  10. Figueredo G, Chalchat JC, Al Juhaimi FY, Özcan MM (2012) Effect of harvest years on chemical composition of essential oil of bitter fennel (Foeniculum vulgare subsp. piperitum) leaves. Asian J Chem 24:2228–2230Google Scholar
  11. Ge P, Ma C, Wang S, Gao L, Li X, Guo G, Ma W, Yan Y (2012) Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress. Anal Bioanal Chem 402:1297–1313CrossRefPubMedGoogle Scholar
  12. Johnson ER, McKay DB (1999) Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase. RNA 5:1526–1534CrossRefPubMedPubMedCentralGoogle Scholar
  13. Komatsu S, Han C, Nanjo Y, Altaf-Un-Nahar M, Wang K, He D, Yang P (2013a) Label-free quantitative proteomic analysis of abscisic acid effect in early-stage soybean under flooding. J Proteome Res 12:4769–4784CrossRefPubMedGoogle Scholar
  14. Komatsu S, Nanjo Y, Nishimura M (2013b) Proteomic analysis of the flooding tolerance mechanism in mutant soybean. J Proteomics 79:231–250CrossRefPubMedGoogle Scholar
  15. Kurek I, Chang TK, Bertain SM, Madrigal A, Liu L, Lassner MW, Zhu G (2007) Enhanced thermostability of Arabidopsis Rubisco activase improves photosynthesis and growth rates under moderate heat stress. Plant Cell 19:3230–3241CrossRefPubMedPubMedCentralGoogle Scholar
  16. Lannoo N, Van Damme EJ (2015) Review/N-glycans: the making of a varied toolbox. Plant Sci 239:67–83CrossRefPubMedGoogle Scholar
  17. Li L, Nelson CJ, Trösch J, Castleden I, Huang S, Millar AH (2017) Protein degradation rate in Arabidopsis thaliana leaf growth and development. Plant Cell 29:207–228CrossRefPubMedPubMedCentralGoogle Scholar
  18. Marino SD, Gala F, Borbone N, Zollo F, Vitalini S, Visioli F, Iorizzi M (2007) Phenolic glycosides from Foeniculum vulgare fruit and evaluation of antioxidative activity. Phytochemistry 68:1805–1812CrossRefPubMedGoogle Scholar
  19. Mohamed M, Abdu M (2004) Growth and oil production of fennel: effect of irrigation and organic fertilization. Bio Agric Hortic 22:31–39CrossRefGoogle Scholar
  20. Mustafa G, Komatsu S (2014) Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress. Front Plant Sci 5:627CrossRefPubMedPubMedCentralGoogle Scholar
  21. Ozcan MM, Chalchat JC, Arslan D, Ate A, Unver A (2006) Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation. J Med Food 9:552–561CrossRefPubMedGoogle Scholar
  22. Piccaglia R, Marotti M (2001) Characterization of some Italian types of wild fennel (Foeniculum vulgare Mill.). J Agric Food Chem 49:239–244CrossRefPubMedGoogle Scholar
  23. Qureshi I, Qadir S, Zolla L (2007) Proteomics-based dissection of stress-responsive pathways in plants. J Plant Physiol 16:1239–1260CrossRefGoogle Scholar
  24. Radchuk V, Borisjuk L (2014) Physical, metabolic and developmental functions of the seed coat. Front Plant Sci 5:e510CrossRefGoogle Scholar
  25. Raines CA, Paul MJ (2006) Products of leaf primary carbon metabolism modulate the developmental programme determining plant morphology. J Exp Bot 57:1857–1862CrossRefPubMedGoogle Scholar
  26. Sade B, Soylu S, Yetim E (2011) Drought and oxidative stress. Afr J Biotechnol 10:11102–11109CrossRefGoogle Scholar
  27. Senatore F, Oliviero F, Scandolera E, Taglialatela-Scafati O, Roscigno G, Zaccardelli M, De Falco E (2013) Chemical composition, antimicrobial and antioxidant activities of anethole-rich oil from leaves of selected varieties of fennel [Foeniculum vulgare Mill. ssp. vulgare var. azoricum (Mill.) Thell]. Fitoterapia 90:214–219CrossRefPubMedGoogle Scholar
  28. Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Hammouda FM, Abdel-Rahman FH, Saleh MA (2011) Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules 16:1366–1377CrossRefPubMedGoogle Scholar
  29. Stefanini MB, Ming LC, Marques MOM, Facanali R, Meireles MAA, Moura LS, Marchese JA, Sousa LA (2006) Essential oil constituents of different organs of fennel (Foeniculum vulgare var. vulgare). Rev Bras Plantas Med 8:193–198Google Scholar
  30. Tahaei A, Soleymani A, Shams M (2016) Seed germination of medicinal plant, fennel (Foeniculum vulgare Mill), as affected by different priming techniques. Appl Biochem Biotechnol 180:26–40CrossRefPubMedGoogle Scholar
  31. Templer SE, Ammon A, Pscheidt D, Ciobotea O, Schuy C, McCollum C, Sonnewald U, Hanemann A, Förster J, Ordon F, von Korff M, Voll LM (2017) Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense. J Exp Bot 68:1697–1713CrossRefPubMedPubMedCentralGoogle Scholar
  32. Timabud T, Yin X, Pongdontri P, Komatsu S (2015) Gel-free/label-free proteomic analysis of developing rice grains under heat stress. J Proteom 133:1–19CrossRefGoogle Scholar
  33. Urban MO, Vašek J, Klíma M, Krtková J, Kosová K, Prášil IT, Vítámvás P (2017) Proteomic and physiological approach reveals drought-induced changes in rapeseeds: Water-saver and water-spender strategy. J Proteom 152:188–205CrossRefGoogle Scholar
  34. Usadel B, Nagel A, Thimm O, Redestig H, Blaesing OE, Palacios-Rofas N, Selbig J, Hannemann J, Piques MC, Steinhauser D, Scheible WR, Gibon Y, Morcuende R, Weicht D, Meyer S, Stitt M (2005) Extension of the visualization tool MapMan to allow statistical analysis of arrays, display of corresponding 31 genes, and comparison with known databases. Plant Physiol 138:1195–1204CrossRefPubMedPubMedCentralGoogle Scholar
  35. Usadel B, Poree F, Nagel A, Lohse M, Czedik-Eysenberg A, Stitt M (2009) A guide to using MapMan to visualize and compare Omics data in plants: a case study in the crop species, Maize. Plant Cell Environ 32:1211–1229CrossRefPubMedGoogle Scholar
  36. Yrjönen T, Eeva M, Kauppila TJ, Martiskainen O, Summanen J, Vuorela P, Vuorela H (2016) Profiling of coumarins in Peucedanum palustre (L.) Moench populations growing in Finland. Chem Biodivers 13:700–709CrossRefPubMedGoogle Scholar
  37. Zahid NY, Abbasi NA, Hafiz IA, Ahmad Z (2009) Genetic diversity of indigenous fennel germplasm in Pakistan assessed by RAPD marker. Pak J Bot 41:1759–1767Google Scholar

Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  • Ehsaneh Khodadadi
    • 1
    • 2
  • Akiko Hashiguchi
    • 3
  • Barat Ali Fakheri
    • 2
  • Saeed Aharizad
    • 4
  • Abbasali Emamjomeh
    • 2
  • Majid Norouzi
    • 4
  • Setsuko Komatsu
    • 1
  1. 1.Faculty of Environmental and Information SciencesFukui University of TechnologyFukuiJapan
  2. 2.Department of Plant Breeding and BiotechnologyUniversity of ZabolZabolIran
  3. 3.Faculty of MedicineUniversity of TsukubaTsukubaJapan
  4. 4.Department of Plant Breeding and BiotechnologyUniversity of TabrizTabrizIran

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