Differential expression of Sub1A loci in rice under submergence
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Two rice land races (Meghi and Bhashakalmi) earlier reported to be submergence tolerant were experimented for differential expression of Sub1A loci under induced submergence with reference to FR13A and IR42 as tolerant and susceptible genotypes, respectively. Two weeks old seedlings were subjected to complete submergence for another 2 weeks and total transcripts were collected at the end of each week for expression study. Relative expression of the said loci was studied with respect to constitutive expression of Ubiquitin 5 for the studied lines. Up regulation of Sub1A loci relative to control was recorded for all the studied lines except IR42. Both the land races showed enhanced expression of Sub1A under induced submergence than IR42 but less than FR13A. After 7 days of complete submergence Meghi showed its higher expression than both FR13A and Bhashakalmi, which was not continued after 14 days as the expression of Sub1A in FR13A exceeded both the Meghi and Bhashakalmi. At the end of recovery period of 7 days, the expression of Sub1A were gradually decreased for all the genotypes. The expression profile of the tested landraces documented in this investigation support their superior submergence tolerance as recorded in our earlier report for physiological and biochemical screening under induced submergence.
KeywordsRice Land race Submergence Sub1A Differential expression
The authors thank Department of Science and Technology (DST-INSPIRE programme, INSPIRE reference number - IF140224) and Department of Biotechnology for financial support.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Biswas T, Das A, Bhattacharyya S (2012) Microsatellite marker based diversity analysis for submergence tolerance in some Bengal landraces of rice (Oryza sativa L.). J Trop Agric 50(1–2):67–71Google Scholar
- Deb D (2005) Seeds of tradition and seeds of future (folk rice varieties of Eastern India). Foundation for Science, Technology and Ecology, New DelhiGoogle Scholar
- Goswami S, Kar RK, Paul A, Dey N (2017a) Study of selected biochemical parameters related to submergence tolerance in rice (Oryza sativa L.) with special reference to land races and wild species. Res J Chem Environ 21(11):29–38Google Scholar
- Mishra PK, Sinha AK (2012) Rice diversity in Bankura district of West Bengal (India). Biosci Discov 3(3):284–287Google Scholar
- Singh N, Dang TT, Vergara GV, Pandey DM, Sanchez D, Neeraja CN, Septiningsih EM, Mendioro M, Tecson-Mendoza EM, Ismail AM, Mackill DJ, Heuer S (2010) Molecular marker survey and expression analyses of the rice submergence tolerance gene SUB1A. Theor Appl Genet 121:1441–1453CrossRefPubMedGoogle Scholar