Anti-VEGFR2 nanobody expression in lettuce using an infectious Turnip mosaic virus vector

  • Malihe Mirzaee
  • Mokhtar Jalali-Javaran
  • Ahmad Moieni
  • Sirous Zeinali
  • Mahdi Behdani
  • Masoud Shams-Bakhsh
  • Mostafa Modarresi
Original Article
  • 46 Downloads

Abstract

Angiogenesis plays an important role in tumor growth and metastasis of cancer. Vascular endothelial growth factor is the key regulator in stimulating angiogenesis. The VEGF activity is mediated by binding to its cell-surface receptors, mainly VEGFR2. Therefore, inhibition of the VEGF/VEGFR2 interaction by antibodies is investigated as a therapeutic strategy in cancer therapy. Here, we describe transient expression of an anti-VEGFR2 nanobody (3VGR19) by a viral vector based on Turnip mosaic virus in lettuce (Lactuca sativa L.). RT-PCR analysis demonstrated the 3VGR19 transcript expression. Western blot analysis showed the 3VGR19 protein expression with an expected molecular mass of ~15 kDa and based on the ELISA results, the expression level of 3VGR19 was 8 μg/g of leaf fresh weight. Taken together, recombinant 3VGR19 could be efficiently expressed in lettuce leaves and TuMV-based expression system would be an appropriate platform for production of recombinant proteins in lettuce plants.

Keywords

Angiogenesis Lettuce Nanobody Transient expression TuMV 

Abbreviations

VEGFR2

Vascular endothelial growth factor receptor 2

TuMV

Turnip mosaic virus

CaMV

Cauliflower mosaic virus

GFP

Green fluorescent protein

Notes

Acknowledgements

The authors gratefully thank Prof. Shyi-Dong Yeh for kindly providing the TuMV-based expression vector p35STuMVGFPHis. We acknowledge the skilled technical assistance of Dr. S. M. Nassaj-Hosseini.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2017

Authors and Affiliations

  • Malihe Mirzaee
    • 1
  • Mokhtar Jalali-Javaran
    • 1
  • Ahmad Moieni
    • 1
  • Sirous Zeinali
    • 2
  • Mahdi Behdani
    • 3
  • Masoud Shams-Bakhsh
    • 4
  • Mostafa Modarresi
    • 1
  1. 1.Department of Plant Breeding and Biotechnology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Department of Molecular MedicinePasteur Institute of IranTehranIran
  3. 3.Venom and Biotherapeutics Molecules Lab., Biotechnology Department, Biotechnology Research CenterPasteur Institute of IranTehranIran
  4. 4.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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