Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for p-coumaric acid production
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Phototrophic cyanobacteria may be considered as an alternative host for producing numerous bioactive compounds. We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 ± 1.5 μg of p-coumaric acid per 108 cells (0.36 mg L−1). Additionally, our study is the first report to show the biotransformation of tyrosine to p-coumaric acid reaching a maximum 2.4-fold increase when 0.5 mM tyrosine was supplemented to the growth medium. Liquid chromatography-mass spectrometry analysis revealed the occurrence of diverse patterns of metabolites under different concentrations of supplemented tyrosine, suggesting that it is used in additional metabolic pathways.
KeywordsBiotransformation p-Coumaric acid LC–MS analysis Synechocystis PCC 6803 Tyrosine
Liquid chromatography–mass spectrometry
Tandem mass spectrometry
Partial least squares discriminant analysis
This work was financially supported by the 90th anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund), an overseas research experience scholarship for graduate students, the Chulalongkorn Academic Advancement into Its 2nd Century Project, Thailand Research Fund IRG578008, the Asahi Glass Foundation, the Swedish Energy Agency, and the KA Wallenberg foundation.
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