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Journal of Plant Biochemistry and Biotechnology

, Volume 27, Issue 1, pp 118–122 | Cite as

Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for p-coumaric acid production

  • S. Tantong
  • N. Nuengchamnong
  • S. Kumphune
  • A. Incharoensakdi
  • P. LindbladEmail author
  • S. SirikantaramasEmail author
Short Communication
  • 271 Downloads

Abstract

Phototrophic cyanobacteria may be considered as an alternative host for producing numerous bioactive compounds. We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 ± 1.5 μg of p-coumaric acid per 108 cells (0.36 mg L−1). Additionally, our study is the first report to show the biotransformation of tyrosine to p-coumaric acid reaching a maximum 2.4-fold increase when 0.5 mM tyrosine was supplemented to the growth medium. Liquid chromatography-mass spectrometry analysis revealed the occurrence of diverse patterns of metabolites under different concentrations of supplemented tyrosine, suggesting that it is used in additional metabolic pathways.

Keywords

Biotransformation p-Coumaric acid LCMS analysis Synechocystis PCC 6803 Tyrosine 

Abbreviations

DAHPS

3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase

ESI

Electrospray ionization

LCMS

Liquid chromatographymass spectrometry

MS/MS

Tandem mass spectrometry

m/z

Mass-to-charge ratio

PAL

Phenylalanine ammonia-lyase

PLS-DA

Partial least squares discriminant analysis

RT

Retention time

TAL

Tyrosine ammonia-lyase

Notes

Acknowledgements

This work was financially supported by the 90th anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund), an overseas research experience scholarship for graduate students, the Chulalongkorn Academic Advancement into Its 2nd Century Project, Thailand Research Fund IRG578008, the Asahi Glass Foundation, the Swedish Energy Agency, and the KA Wallenberg foundation.

Supplementary material

13562_2017_416_MOESM1_ESM.tif (1.4 mb)
Fig. S1 The in vivo production of p-coumaric acid from Synechocystis PCC 6803 expressing TAL controlled by Ptrc1Ocore or Ptrc1O. The engineered cells of Synechocystis were grown in BG-11 medium with 25 µg mL−1 kanamycin under 50 μEm−2s−1 white light, 30 °C and 120 rpm until OD730 reached 0.5 to 0.6. The cells were collected for p-coumaric acid detection by LC-MS (6540 QTOF-LC/MS, Agilent Technologies, USA) equipped with dual electrospray ionization (ESI) operated in negative mode. The extracted ion chromatogram showed the molecular weight of the p-coumaric acid standard = 163.02[M-H]. The asterisks show the compounds produced from engineered Synechocystis PCC 6803 cells (TIFF 1419 kb)

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2017

Authors and Affiliations

  • S. Tantong
    • 1
    • 2
  • N. Nuengchamnong
    • 3
  • S. Kumphune
    • 4
  • A. Incharoensakdi
    • 2
  • P. Lindblad
    • 5
    Email author
  • S. Sirikantaramas
    • 2
    • 6
    Email author
  1. 1.Program in Biotechnology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Department of Biochemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Science Laboratory Centre, Faculty of ScienceNaresuan UniversityPhitsanulokThailand
  4. 4.Department of Medical Technology, Faculty of Allied Health SciencesNaresuan UniversityPhitsanulokThailand
  5. 5.Microbial Chemistry, Department of Chemistry –ÅngströmUppsala UniversityUppsalaSweden
  6. 6.Omics Sciences and Bioinformatics Center, Faculty of ScienceChulalongkorn UniversityBangkokThailand

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