Journal of Plant Biochemistry and Biotechnology

, Volume 26, Issue 4, pp 425–435 | Cite as

Molecular stacking of wide compatibility gene, S5 n and elongated uppermost internode (eui) gene into IR 58025B, an elite maintainer line of rice

  • Rahul Priyadarshi
  • Hari Prasad Subramanyam Arremsetty
  • Akhilesh Kumar Singh
  • Durga Khandekar
  • Kandasamy Ulaganathan
  • Vinay Shenoy
Original Article


One of the most widely used and stable wild-abortive cytoplasmic male sterile (WA-CMS) line is IR 58025A. We had earlier transferred the gene (eui1) for elongated uppermost internode into the elite WA-CMS line named IR 58025eB with the help of markers. To overcome incomplete panicle exsertion in the WA-CMS line and better exploitation of indica/japonica heterosis, we have stacked a major gene for wide-compatibility (S5 n ) into IR 58025eB through marker-assisted breeding. SSR markers (RM5970) tightly linked to eui1 gene and (S5-InDel) functional maker of S5 n gene were used for foreground selection, while ninety-one microsatellite markers polymorphic between IR 58025eB and donor Dular (S5 n ) were utilized for background selection at each backcross generation and backcrossing was continued till BC3 generation. Phenotypic observation previous to background selection at each generation provided supplementary information for identifying the novel recombinants and the fast-tracked recurrent parent genome recovery which ranged from 94.50 to 96.70% in selected BC3F4 plants. At BC3F5 generation, seven stable, backcross-derived lines possessing S5 n and eui genes, high yield, and long-slender grain type have been identified; their maintainer ability was confirmed through test crosses. These improved lines are being converted to CMS lines by marker-assisted backcrossing.


Hybrid rice IR 58025eB Maintainer line Wide compatibility Elongated uppermost internode Marker-assisted backcross breeding 





Cytoplasmic male sterile


Gibberellic acid


Elongated uppermost internode gene


Simple sequence repeat


Wide compatibility


Marker-assisted backcross breeding



We thank our Executive Director and Director Dr. Usha, B. Zehr for providing all the facilities for execution of this research work and all the encouragement and support. I would like to thanks Dr. R. M. Sundaram, Principal Scientist (Biotechnology), ICAR-Indian Institute of Rice Research, Hyderabad for providing valuable suggestions in the preparation of manuscript.


This study was funded by Barwale Foundation, Hyderabad, India.

Compliance with ethical standards

Conflict of interest

These authors do not have any conflict of interest.

Supplementary material

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Supplementary material 5 (JPEG 77 kb)


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Copyright information

© Society for Plant Biochemistry and Biotechnology 2017

Authors and Affiliations

  • Rahul Priyadarshi
    • 1
    • 2
    • 3
    • 4
  • Hari Prasad Subramanyam Arremsetty
    • 2
  • Akhilesh Kumar Singh
    • 3
    • 5
  • Durga Khandekar
    • 3
    • 6
  • Kandasamy Ulaganathan
    • 4
  • Vinay Shenoy
    • 3
    • 7
  1. 1.International Rice Research Institute, South Asia HubInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT)HyderabadIndia
  2. 2.ICAR-Indian Institute of Rice ResearchHyderabadIndia
  3. 3.Barwale Foundation Research CentreHyderabadIndia
  4. 4.Osmania UniversityHyderabadIndia
  5. 5.Rasi Seeds Private LimitedHyderabadIndia
  6. 6.AgriGenome Labs Private LimitedHyderabadIndia
  7. 7.Kaveri Seed Company LimitedSecunderabadIndia

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