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Asymbiotic seed germination and in vitro seedling development of Cymbidium aloifolium (L.) Sw.: a multipurpose orchid

  • Chitta Ranjan DebEmail author
  • Aolemla Pongener
Original Article

Abstract

Cymbidium aloifolium is a multipurpose economically important epiphytic orchid grows on tree trunk in the primary forests. Its population in natural habitat is downsized due to different anthropogenic activities. A successful attempt was made for asymbiotic immature embryo culture and in vitro mass scale production of plantlets. For successful culture initiation seed pods of various developmental ages, various nutrient media, sucrose concentrations, different quality and quantity of plant growth regulators were surveyed. Immature embryos of 9 months after pollination was successfully germinated on MS medium containing sucrose (2%) (w/v) and α-naphthalene acetic acid (NAA) and benzyl adenine (BA) (3 and 6 μM respectively in combination) within 45 days of culture where 90% germination was recorded. The germinated seeds formed PLBs on the optimum germination medium within two passages. The protocorm like bodies (PLBs) differentiated into rooted plantlets within 3 weeks on regeneration medium containing sucrose (3%), casein-hydrolysate (0.1 gl−1) and BA 3 μM. Amongst the three media studied, optimum regeneration was registered on MS medium where as many as 12 shoot buds developed per explants per subculture of 4 weeks duration. The well rooted plantlets of 6–7 cm long with 3–4 roots were hardened in vitro 3–4 weeks before they were transferred to potting mix. The potted plants were exposed to full sunlight periodically and watered at regular interval. About 70–80% transplants survived after 2 months of potting.

Keywords

Asymbiotic seed germination Cymbidium aloifolium Immature embryo culture Medicinal orchid Micropropagation 

Abbreviations

BA

Benzyl adenine

CH

Casein hydrolysate

IAA

Indole 3 acetic acid

KN

Kinetin

MAP

Months after pollination

MS

Muraghige and Skoog

NAA

α-naphthalene acetic acid

PGRs

Plant growth regulators

PLBs

Protocorm like bodies

TDZ

Thidiazuron

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Copyright information

© Optical Society of India 2011

Authors and Affiliations

  1. 1.Department of BotanyNagaland UniversityLumamiIndia

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