Biomedical Engineering Letters

, Volume 8, Issue 4, pp 393–398 | Cite as

Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

  • Saeed Farzamfar
  • Majid SalehiEmail author
  • Arian Ehterami
  • Mahdi Naseri-Nosar
  • Ahmad Vaez
  • Amir Hassan Zarnani
  • Hamed Sahrapeyma
  • Mohammad-Reza Shokri
  • Mehdi Aleahmad
Original Article


This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via decellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM was seeded with MenSCs at the density of 3 × 104 cells/cm2 and implanted onto a rat’s 1.50 × 1.50 cm2 full-thickness excisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSC-seeded DAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicated that the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.


Amniotic membrane Wound healing Menstrual blood-derived stem cells 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

Animal experiments were approved by the ethical committee of Shahroud University of Medical Sciences and were carried out in accordance with the university’s guidelines.


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Copyright information

© Korean Society of Medical and Biological Engineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Saeed Farzamfar
    • 1
  • Majid Salehi
    • 2
    • 3
    Email author
  • Arian Ehterami
    • 2
  • Mahdi Naseri-Nosar
    • 3
  • Ahmad Vaez
    • 1
  • Amir Hassan Zarnani
    • 4
    • 5
  • Hamed Sahrapeyma
    • 6
  • Mohammad-Reza Shokri
    • 7
  • Mehdi Aleahmad
    • 4
  1. 1.Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  2. 2.Department of Tissue Engineering, School of MedicineShahroud University of Medical SciencesShahroudIran
  3. 3.Tissue Engineering and Stem Cells Research CenterShahroud University of Medical SciencesShahroudIran
  4. 4.Department of Immunology, School of Public HealthTehran University of Medical SciencesTehranIran
  5. 5.Reproductive Immunology Research Center, Avicenna Research InstituteACECRTehranIran
  6. 6.Department of Biomedical Engineering, Science and Research BranchIslamic Azad UniversityTehranIran
  7. 7.Department of Immunology, School of MedicineIran University of Medical SciencesTehranIran

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