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Endosulfan Induces Embryotoxicity in the Marine Medaka Oryzias javanicus

  • Manoharan Saravanan
  • Sang-Eun Nam
  • Jae-Sung RheeEmail author
Article
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Abstract

Objective

The present study aimed to investigate the embryotoxicity of endosulfan (ES) on marine medaka Oryzias javanicus embryos using several toxicity endpoints such as measurements of oxidative stress, DNA damage, cell death, and AChE enzyme activity upon different concentrations of waterborne ES.

Methods

Fertilized marine medaka embryos (3 hpf) were exposed to three concentrations of ES (86.2, 172.4, and 862 µg L−1). Oxidative stress parameters [e.g. intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH)], nitric oxide (NO) production, DNA damage, cell death, and acetylcholinesterase (AChE) activity were analyzed.

Results

The highest concentration 862 µg L−1 of ES significantly increased the intracellular ROS, MDA, GSH, and NO in the embryos compared to control groups. Endosulfan increased DNA damage and cell death in the marine medaka embryos during post fertilization period. In the case of AChE, a significant inhibition was detected in the 862 µg L−1 ES-treated medaka embryo. In addition, intracellular GSH level was increased by 172.2 µg L−1 ES exposure in medaka embryos.

Conclusion

Our results suggest that ES may induce strong cell death through increase of oxidative stress and DNA damage. Inhibition of AChE would represent potential neurotoxicity on cholinergic system of marine medaka embryos. The parameters tested in this study would be a useful biomarker for risk assessment of ES as an early signal of its toxicity.

Keywords

Endosulfan Marine medaka Embryo toxicity Oxidative stress DNA damage Cell death 

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Notes

Acknowledgements

Publication of this article was co-sponsored by the ToxEHS.

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Copyright information

© The Korean Society of Environmental Risk Assessment and Health Science and Springer 2019

Authors and Affiliations

  • Manoharan Saravanan
    • 1
    • 2
  • Sang-Eun Nam
    • 1
  • Jae-Sung Rhee
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Marine Science, College of Natural SciencesIncheon National UniversityIncheonRepublic of Korea
  2. 2.Research Institute of Basic SciencesIncheon National UniversityIncheonRepublic of Korea
  3. 3.Institute of Green Environmental Research CenterIncheonRepublic of Korea

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