The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat
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Lead is considered one of the major causes of infertility among occupational workers in manufacturing companies such as electric storage batteries, glass and ceramic wares. This study was carried out to confirm the effect of Gallic acid as an antioxidant on Lead acetate treated male albino rats.
Twenty albino rats weighing between 140 g and 200 g were divided equally into four groups. Group A served as the control, group B was treated with 60 mg/kg body weight of Lead acetate, group C received 50 mg/kg body weight of Gallic acid and group D received both Lead acetate and Gallic acid for 14 days.
The result showed slight increase in the body weights and testes of group B and D compared to the control group and there was a slight decrease in group C. Rats in group B showed a significant increase in MDA (3224.63±89.38 unit/mg protein) but a slight increase was observed in the level of MDA (1537.70± 53.92 unit/mg protein) in group D. The level of Lactate Dehydrogenase (LDH) in group B was 338.75±18.87 nmol min mL, which was the highest among the groups and significant.
Lead acetate is toxic to the gonad, it causes degeneration and reduction in spermatogenic activity in seminiferous tubules. However, the effect of Lead on the male reproductive organ can be reverse significantly by an antioxidant (gallic acid) due to its ability to scavenge for oxidative stress caused by Lead.
KeywordsLead acetate Spermatogenesis Toxicity Gallic acid Testes
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- 1.Vigeh, M., Smith, D. R. & Hsu, P.–C. How does lead induce male infertility? Iran. J. Reprod. Med. 9, 1–8 (2011).Google Scholar
- 2.Sallmén, M. International journal of occupational medicine and environmental health. Int. J. Occup. Med. Environ. Healt. 14, 219–222 (2001).Google Scholar
- 3.Adebayo Kehinde, B. & Ayoola Isaac, J. Effects of Heat and Lead on the Reproductive System of Male Wistar Rat. Anat. Sci. J. 11, 99–106 (2014).Google Scholar
- 4.Olayemi, F. O. A review on some causes of male infertility. Afr. J. Biotechnol. 9, 2834–2842 (2002).Google Scholar
- 7.AIT Hamadouche, N. Reproductive toxicity of lead acetate in adult male rats. Am. J. Sci. Res. 3, 38–50 (2009).Google Scholar
- 12.Fuchs–Tarlovsky, V. Role of antioxidants in cancer therapy. Nutritio. 29, 15–21 (2013).Google Scholar
- 14.Jin, L. et al. Gallic Acid Reduces Blood Pressure and Attenuates Oxidative Stress and Cardiac Hypertrophy in Spontaneously Hypertensive Rats. Sci. Rep. 7, doi: 10. 1038/s41598–017–15925–1 (2017).Google Scholar
- 15.Hassan, A. I. & Alam, S. S. Evaluation of mesenchymal stem cells in treatment of infertility in male rats. Stem Cell Res. Ther. 5, doi: 10.1186/scrt521 (2014).Google Scholar
- 17.Sujatha, K., Srilatha, C. H., Rao, T. S. C. & Amaravathi, P. Ultrastructural and histopathological studies in lead acetate induced neurotoxicity in wistar albino rats and its amelioration with Ocimum sanctum (os) a leaf extract. Int. J. Pharma Bio Sci. 2, 295–304 (2011).Google Scholar
- 18.Canbek, M. et al. The effect of gallic acid on kidney and liver after experimental renal ischemia/reperfusion injury in the rats. African J. Pharm. Pharmacol. 5, 1027–1033 (2011).Google Scholar
- 19.Kobyliak, N. M. et al. Antioxidative effects of cerium dioxide nanoparticles ameliorate age–related male infertility: optimistic results in rats and the review of clinical clues for integrative concept of men health and fertility. EPMA J. 6, doi: 10.1186/s13167–015–0034–2 (2015).Google Scholar
- 21.Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951).Google Scholar