Association of adiponectin gene polymorphisms and their haplotypes with type 2 diabetes and related metabolic traits in an Iranian population

  • Hamid Reza Joshaghani
  • Parviz Kokhaei
  • Mehdi Barati
  • Abbas Pakdel
  • Ghorban Mohammadzadeh
  • Navid Bandegi
  • Ahmad Reza BandegiEmail author
Original Article



Adiponectin is an adipocyte-secreted protein that contributes to glucose homeostasis. Contradictory reports are available on single nucleotide polymorphisms (SNPs) in the adiponectin gene and the risk of type 2 diabetes (T2D). We investigate the association of adiponectin gene SNPs (+45T/G and +276G/T) with serum adiponectin, insulin resistance, lipid profile, and T2D risk in an Iranian population.


The +45T/G and +276G/T SNPs were genotyped in 211 non-familial T2D patients and 202 non-diabetic subjects by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan probe, respectively.


T2D was associated with a decrease in serum adiponectin level. The G allele and the GG and TG genotypes of +45T/G SNP were more abundant than the T allele and the TT genotype in T2D patients compared with controls (p < 0.001). The risk of T2D in individuals with the GG and TG genotypes of +45T/G SNP was 4 and 2 times more than that with the TT genotype, respectively. There was no statistically significant difference in the frequencies of allele and genotype of +276G/T SNP between the control and T2D groups. The presence of +45G/+276G haplotype was associated with an increased risk of T2D (OR = 2.01, 95% CI = 1.34–3.03, p = 0.04).


Therefore, our results showed that +45T/G SNP is associated with the risk of T2D higher than +276G/T SNP in the studied population.


Adiponectin Type 2 diabetes mellitus Gene polymorphism Iran 



We are most grateful to Vice Chancellor for Research Centers, Semnan University of Medical Sciences, for providing research facilities.

Funding information

This work was supported by a grant (No. 271) from Semnan University of Medical Sciences and Golestan University of Medical Sciences.

Compliance with ethical standards

Human and animal rights

The study has been approved by the appropriate local ethics committee at the Semnan University of Medical Sciences and has been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Ethical standard

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Semnan University of Medical Sciences research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Research Society for Study of Diabetes in India 2020

Authors and Affiliations

  1. 1.Biochemistry & Metabolic Disorders Research CenterGolestan University of Medical SciencesGorganIran
  2. 2.Cancer Research CenterSemnan University of Medical SciencesSemnanIran
  3. 3.Nervous System Stem Cells Research CenterSemnan University of Medical SciencesSemnanIran
  4. 4.Department of Biochemistry, Faculty of MedicineSemnan University of Medical SciencesSemnanIran
  5. 5.Hyperlipidemia Research Center, Dept. of Clinical Biochemistry, Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  6. 6.Faculty of MedicineBirjand University of Medical SciencesBirjandIran
  7. 7.Research Center of PhysiologySemnan University of Medical SciencesSemnanIran

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