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Comparison of salivary nitric oxide and epidermal growth factor level between diabetic patients and healthy individuals

  • Hamid Reza Abdolsamadi
  • Fatemeh Rezaei
  • Mohammad Taghi Goodarzi
  • Abbas Moghimbeigi
  • Mina Jazaeri
  • Soheila Asadi
  • Fatemeh Ahmadi-MotamayelEmail author
Original Article

Abstract

The purpose of the current study was to compare salivary Nitric oxide and epidermal growth factor level in diabetic patients and healthy individuals. In this paired-control study 20 patients with type 1 diabetes mellitus, 20 patients with type 2 diabetes mellitus and 20 age and sex matched healthy subjects participated. The level of salivary Nitric oxide was increased in type 1 and 2 diabetic patients in comparison to control individuals (P = 0.037 and P = 0.058), respectively. Epidermal growth factor concentration was higher in type 1 and 2 diabetic patients compare to healthy ones, (P = 0.037 and P = 0.005) respectively. There was no significant difference between salivary Nitric oxide and Epidermal growth factor level regarding to the type of diabetes (P > 0.05). Significant statistical correlation was found between Nitric oxide and Epidermal growth factor levels and the fasting blood glucose and HbA1c (P < 0.001). Conclusions: Salivary Nitric oxide and Epidermal growth factor levels were higher in type 1 and type 2 diabetic patients and there was correlation with severity of disease.

Keywords

Type 1 diabetes mellitus Type 2 diabetes mellitus Biomarker 

Notes

Acknowledgments

This article is a part of thesis supported by Hamadan University of Medical Sciences. We would like to thank Deputy of Education as well as Deputy of Research and Technology of Hamadan University of Medical Sciences for financial support of this study. The authors would like to thank Dr Zeynab Parvizi for English editing of this article.

Conflict of Interests

The authors declare that they have no conflicts of interest.

References

  1. 1.
    Power A. Diabetes mellitus. In: Fauci AS, Kasper DL, Longo DL, Braunwald E, Hauser SL, Jameson L, et al., editors. Harrison’s principls of internal medicine. 18th ed. New York: McGraw-Hill; 2012. p. 2152–80.Google Scholar
  2. 2.
    Castagnola M, Picciotti PM, Messana I, et al. Potential applications of human saliva as diagnostic fluid. Acta Otorhinolaryngol Ital. 2011;31:347–57.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Liu JDY. Saliva: a potential media for disease diagnostics and monitoring. Oral Oncol. 2012;18.Google Scholar
  4. 4.
    Marletta MA. Nitric oxide synthase structure and mechanism. J Biol Chem. 1993;269:12231–4.Google Scholar
  5. 5.
    Sunitha M, Shanmugam S. Evaluation of salivary nitric oxide levels in oral mucosal diseases: a controlled clinical trial. Indian J Dent Res. 2006;17:117–20.PubMedCrossRefGoogle Scholar
  6. 6.
    Nagy G, Koncz A, Telarico T, et al. Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus. Arthritis Res Ther. 2010;12:210.PubMedPubMedCentralCrossRefGoogle Scholar
  7. 7.
    Wilsher ML, Fergusson W, Milne D, et al. Exhaled nitric oxide in sarcoidosis. Thorax. 2005;60:967–70.PubMedPubMedCentralCrossRefGoogle Scholar
  8. 8.
    Schildge J. Nitric oxide in exhaled breath of patients with interstitial lung diseases. Pneumologie. 2011;65:143–8.PubMedCrossRefGoogle Scholar
  9. 9.
    Yalcin AD, Gorczynski RM, Parlak GE, et al. Total antioxidant capacity, hydrogen peroxide, malondialdehyde and total nitric oxide concentrations in patients with severe persistent allergic asthma: its relation to omalizumab treatment. Clin Lab. 2012;58:89–96.PubMedGoogle Scholar
  10. 10.
    Farhad AR, Razavi S, Jahadi S, et al. Use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide in periapical inflammation. J Oral Sci. 2011;53:225–30.PubMedCrossRefGoogle Scholar
  11. 11.
    Kim KA, Shin YJ, Kim JH, et al. Dysfunction of endothelial progenitor cells under DM conditions and its underlying mechanisms. Arch Pharm Res. 2012;35:223–34.PubMedCrossRefGoogle Scholar
  12. 12.
    Shoukry A, Shalaby SM, Abdelazim S, et al. Endothelial nitric oxide synthase gene polymorphisms and the risk of diabetic nephropathy in type 2 diabetes mellitus. Genet Test Mol Biomarkers. 2012;16(6):574–9.PubMedCrossRefGoogle Scholar
  13. 13.
    Abu-Saleh N, Ovcharenko E, Awad H, et al. Involvement of the endothelin and nitric oxide systems in the pathogenesis of renal ischemic damage in an experimental DM model. Life Sci. 2012;17.Google Scholar
  14. 14.
    Maritim AC, Sanders RA, Watkins 3rd JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003;17:24–38.PubMedCrossRefGoogle Scholar
  15. 15.
    Carpenter G, Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216.PubMedCrossRefGoogle Scholar
  16. 16.
    Niall M, Ryan G, O’Brien BM. The effect of epidermal growth factor on wound healing in mice. J Surg Res. 1982;33:164–9.PubMedCrossRefGoogle Scholar
  17. 17.
    Noguchi S, Ohba Y, Oka T. Effect of salivary epidermal growth factor on wound healing of tongue in mice. Am J Physiol. 1991;260:E620–5.PubMedGoogle Scholar
  18. 18.
    Lee HY, Yea K, Kim J, et al. Epidermal growth factor increases insulin secretion and lowers blood glucose in DM mice. J Cell Mol Med. 2008;12:1593–604.Google Scholar
  19. 19.
    Yu H, Sun Z, Cui J, et al. Epidermal growth factor and gastrin on PDX1 expression in experimental type 1 DM rats. Am J Med Sci. 2012;343:141–5.Google Scholar
  20. 20.
    Loukovaara M, Leinonen P, Teramo K, et al. DM pregnancy associated with increased epidermal growth factor in cord serum at term. Obstet Gynecol. 2004;103:240–4.Google Scholar
  21. 21.
    Lao G, Yan L, Yang C, et al. Controlled release of epidermal growth factor from hydrogels accelerates wound healing in DM rats. J Am Podiatr Med Assoc. 2012;102:89–98.Google Scholar
  22. 22.
    Nagy A, Nagashima H, Cha S, et al. Reduced oral wound healing in the NOD mouse model for type 1 autoimmune diabetes and its reversal by epidermal growth factor supplementation. Diabetes. 2001;50:2100–4.PubMedCrossRefGoogle Scholar
  23. 23.
    WHO. The use and interpretation of anthropometry. Geneva: WHO; 1995.Google Scholar
  24. 24.
    Navazesh M, Kumar SK. Measuring salivary flow: challenges and opportunities. J Am Dent Assoc. 2008;139:35S–40S.PubMedCrossRefGoogle Scholar
  25. 25.
    Astaneie F, Afshari M, Mojtahedi A, et al. Total antioxidant capacity and levels of epidermal growth factor and nitric oxide in blood and saliva of insulin-dependent DM patients. Arch Med Res. 2005;36:376–81.PubMedCrossRefGoogle Scholar
  26. 26.
    Oxford GE, Tayari L, Barfoot MD, et al. Salivary EGF levels reduced in DM patients. J Diabetes Complications. 2000;14:140–5.PubMedCrossRefGoogle Scholar
  27. 27.
    Ohashi M, Iwase M, Nagumo M. Elevated production of salivary nitric oxide in oral mucosal diseases. J Oral Pathol Med. 1999;28:355–9.PubMedCrossRefGoogle Scholar
  28. 28.
    Ahdi M, Gerdes V, Hoekstra JB, et al. Diabetes mellitus. Ned Tijdschr Tandheelkd. 2012;119:65–71.PubMedCrossRefGoogle Scholar
  29. 29.
    Mocada S, Palmer R, Higgs EA. Nitric oxide, physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991;43:109–42.Google Scholar
  30. 30.
    Sessa WC, Prichard K, Seyedi N, et al. Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression. Circ Res. 1994;74:349–53.PubMedCrossRefGoogle Scholar
  31. 31.
    Abdollahi M, Zadehkabir R, Rahmat-jirdeh N, et al. Interaction of L-arginine/nitric oxide system with lead acetate on secretion of amylase from isolated rat parotid glands. DARU. 2001;1:50–7.Google Scholar
  32. 32.
    Abou-Seif MA, Youssef A. Evaluation of some biochemical changes in DM patients. Clin Chim Acta. 2004;346:161–70.PubMedCrossRefGoogle Scholar
  33. 33.
    Takeda M, Mori F, Yoshida A, Takamiya A, et al. Constitutive nitric oxide synthase is associated with retinal vascular permeability in early DM rats. Diabetologia. 2001;44:1043–50.PubMedCrossRefGoogle Scholar
  34. 34.
    DeVries AS, Stoenoiu MS, Elger M, et al. Diabetes-induced microvascular dysfunction in the hydronephrotic kidney; role of nitric oxide. Kidney Int. 2001;60:202–10.CrossRefGoogle Scholar
  35. 35.
    Volk T, Loannidis I, Hensel M, et al. Endothelial damage induced by nitric oxide synergism with reactive oxygen species. Biophys Res Commun. 1995;213:1196–203.CrossRefGoogle Scholar
  36. 36.
    Reher VG, Zenobio EG, Costa FO, et al. Nitric oxide levels in saliva increase with severity of chronic periodontitis. J Oral Sci. 2007;49:271–6.PubMedCrossRefGoogle Scholar
  37. 37.
    Das D, Bandyopadhyay D, Bhattacharjee M, et al. Hydroxyl radical is the major causative factor in stress induced gastric ulceration. Free Radic Biol Med. 1997;23:8–18.PubMedCrossRefGoogle Scholar
  38. 38.
    Kasayama S, Ohba Y, Oka T. Epidermal growth factor deficiency associated with diabetes mellitus. Proc Natl Acad Sci U S A. 1989;86:7644–8.PubMedPubMedCentralCrossRefGoogle Scholar
  39. 39.
    Hu Y, Nakagawa Y, Purushotham KR, et al. Functional changes in salivary glands of autoimmune disease-prone NOD mice. Am J Physiol. 1992;263:E607–14.PubMedGoogle Scholar
  40. 40.
    Kim SK, Cuzzort LM, Mskean RK, et al. Effects of diabetes and insulin on a-amylase messenger RNA levels in rat parotid glands. J Dent Res. 1990;69:1500–4.PubMedCrossRefGoogle Scholar
  41. 41.
    Yu H, Sun Z, Cui J, et al. Epidermal growth factor and gastrin on PDX1 expression in experimental type 1. Am J Med Sci. 2012;343:141–5.PubMedCrossRefGoogle Scholar
  42. 42.
    Suarez-Pinzon WL, Yan Y, Power R, et al. Combination therapy with epidermal growth factor and gastrin increases beta-cell mass and reverses hyperglycemia in DM NOD mice. Diabetes. 2005;54:2596–601.PubMedCrossRefGoogle Scholar
  43. 43.
    Knighton D R, Fiegel VD. Growth factor and comprehensive surgical care of DM wounds. Curr Opin Gen Surg. 1993;32–9.Google Scholar
  44. 44.
    Olsen PS, Poulsen SS, Kirkagaard P, et al. Role of submandibular gland saliva and epidermal growth factor in gastrin cytoprotection. Gastroenterology. 1984;87:103–8.PubMedGoogle Scholar
  45. 45.
    Tsang MW, Wong WK, Hung CS, et al. Human epidermal growth factor enhances healing of DM foot ulcers. Diabetes Care. 2003;26:1856–61.PubMedCrossRefGoogle Scholar
  46. 46.
    Zelles T, Purushotham K, Macauley SP, et al. Saliva and growth factors: the fountain of youth resides in us all. J Dent Res. 1995;74:1826–32.PubMedCrossRefGoogle Scholar
  47. 47.
    Sreebny LM, Yu A, Green A, et al. Xerostomia in diabetes mellitus. Diabetes Care. 1992;15:900–4.PubMedCrossRefGoogle Scholar
  48. 48.
    Marotta PS, Fontes T, Armada L, et al. Type 2 diabetes mellitus and the prevalence of apical periodontitis and. J Endod. 2012;38:297–300.PubMedCrossRefGoogle Scholar
  49. 49.
    Hormia M, Thesleff I, Perheentupa J, et al. Increased rate of salivary epidermal growth factor secretion in patients with juvenile periodontitis. Scand J Dent Res. 1993;101:138–44.PubMedGoogle Scholar

Copyright information

© Research Society for Study of Diabetes in India 2014

Authors and Affiliations

  • Hamid Reza Abdolsamadi
    • 1
  • Fatemeh Rezaei
    • 2
  • Mohammad Taghi Goodarzi
    • 3
  • Abbas Moghimbeigi
    • 4
  • Mina Jazaeri
    • 5
  • Soheila Asadi
    • 6
  • Fatemeh Ahmadi-Motamayel
    • 7
    Email author
  1. 1.Dental Research Center - Department of Oral Medicine, School of DentistryHamadan University of Medical SciencesHamadanIran
  2. 2.Department of Oral Medicine, School of DentistryKermanshah University of Medical SciencesKermanshahIran
  3. 3.Research Center for Molecular Medicine, School of MedicineHamadan University of Medical SciencesHamadanIran
  4. 4.Department of Biostatistic, School of Public HealthHamadan University of Medical SciencesHamadanIran
  5. 5.Department of Oral Medicine, School of DentistryHamadan University of Medical SciencesHamadanIran
  6. 6.Department of Biochemistry, School of MedicineHamadan University of Medical SciencesHamadanIran
  7. 7.Research Center for Molecular Medicine, Department of Oral Medicine, Dental SchoolHamadan University of Medical SciencesHamadanIran

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