Enhancing the radiotherapeutic index of gamma radiation on cervical cancer cells by gold nanoparticles
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Nanotechnology has the impending ability to improve the therapeutic potential of drugs and radiation-based treatment approaches for reducing cancerous cell death while curtailing collateral toxicity to non-cancerous cells. Among all metal nanomaterials, gold nanoparticles (AuNPs) are establishing themselves as an excellent radiosensitizer and serve as a multimodal modality due to their unique physicochemical properties. The primordial purpose of the work is to evaluate the synergistic effect and molecular level interaction of gamma (γ) radiation on human cervical cancer cell (HeLa) in the presence of AuNPs. Biocompatible AuNPs in combination with γ-radiation were found to exhibit elated cytotoxic effects on cancer cells as evidenced by cell-based assays. The implication of AuNPs facilitates the minimization of radiation dose employment on cultured cells. As per our experimental evaluation, the modus operandi of dual effectors ascertained that a higher amount of reactive oxygen species (ROS) plays a key role in cellular functionality collapse. In that scenario, it can be concluded that AuNP-mediated radiosensitization proved to be the plausible candidate for preclinical testing in nanoparticle-based radiotherapy.
KeywordsGold nanoparticles Cancer Radiosensitization Ionizing radiation Gamma radiation Oxidative stress
The authors would like to express their sincere gratitude to Dr. Sayan Bhattacharya for providing many conveniences at the Department of Chemical Sciences, IISER-Kolkata. Authors are also thankful to Dr. Abhijit Saha and Dr. Aparna Datta for providing the in vitro gamma irradiation facility to cells at UGC-DAE, CSR Kolkata Centre. The authors would like to acknowledge the faculties of the Department of Molecular Biology & Biotechnology, University of Kalyani, for providing departmental facilities.
Priya Yadav is thankful to University Grant Commission-Department of Atomic Energy, Consortium of Scientific Research, Kolkata (UGC-DAE, CSR, Kolkata) for providing fellowship. The authors gratefully acknowledge UGC-DAE Kolkata (UGC-DAE-CSR-KC/CSR/13/RB-03/0840) and Department of Science and Technology (DST-Nano mission) (SR/NM/NS-1447/2014) for financial support.
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Conflict of interest
The authors declare that they have no conflict of interest.
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