Cellular Oncology

, Volume 36, Issue 1, pp 55–63 | Cite as

Expression of small nucleolar RNAs in leukemic cells

  • Kaisa J. Teittinen
  • Asta Laiho
  • Annemari Uusimäki
  • Juha-Pekka Pursiheimo
  • Attila Gyenesei
  • Olli Lohi
Original Paper



Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells.


The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles.


Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines.


snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.


Leukemia snoRNA SOLiD Nucleolus 



Acute lymphoblastic leukemia


Acute myeloid leukemia


Acute promyeloid leukemia


Burkitt’s lymphoma/leukemia


Differentially expressed


Fold change




Non-protein-coding RNA


Non-small cell lung cancer


Quantitative real-time PCR


Cajal body-specific RNA


Small interfering RNA


Small nuclear RNA


Small nucleolar RNA


Small nucleolar ribonucleoprotein



We thank Mr. Jorma Kulmala for technical assistance. This work was supported by the Academy of Finland Research Council for Health (funding decision number 115260), the Foundation for Paediatric Research in Finland, the Finnish Medical Foundation, the Competitive Research Funding of Tampere University Hospital (grants 9J062, 9K073 and 9M052), the Nona and Kullervo Väre Foundation and the Päivikki and Sakari Sohlberg Foundation. The funding sources had no involvement in the study.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13402_2012_113_MOESM1_ESM.doc (837 kb)
ESM 1 (DOC 837 kb)


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Copyright information

© International Society for Cellular Oncology 2012

Authors and Affiliations

  • Kaisa J. Teittinen
    • 1
  • Asta Laiho
    • 2
  • Annemari Uusimäki
    • 1
  • Juha-Pekka Pursiheimo
    • 2
  • Attila Gyenesei
    • 2
  • Olli Lohi
    • 1
  1. 1.Tampere Center for Child Health ResearchUniversity of Tampere School of Medicine and Tampere University HospitalTampereFinland
  2. 2.Turku Centre for Biotechnology and the Finnish Microarray and Sequencing CentreUniversity of Turku and Åbo Akademi UniversityTurkuFinland

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