Expression of small nucleolar RNAs in leukemic cells
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Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells.
The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles.
Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines.
snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.
KeywordsLeukemia snoRNA SOLiD Nucleolus
Acute lymphoblastic leukemia
Acute myeloid leukemia
Acute promyeloid leukemia
Non-small cell lung cancer
Quantitative real-time PCR
Cajal body-specific RNA
Small interfering RNA
Small nuclear RNA
Small nucleolar RNA
Small nucleolar ribonucleoprotein
We thank Mr. Jorma Kulmala for technical assistance. This work was supported by the Academy of Finland Research Council for Health (funding decision number 115260), the Foundation for Paediatric Research in Finland, the Finnish Medical Foundation, the Competitive Research Funding of Tampere University Hospital (grants 9J062, 9K073 and 9M052), the Nona and Kullervo Väre Foundation and the Päivikki and Sakari Sohlberg Foundation. The funding sources had no involvement in the study.
Conflict of interest
The authors declare no conflict of interest.
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