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Utilisation of tuna condensate waste from the canning industry as a novel substrate for polyhydroxyalkanoate production

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Abstract

Tuna condensate, an organic-rich by-product from the tuna canning industry, was assessed as a substrate for polyhydroxyalkanoate (PHA) production using Cupriavidus necator TISTR 1095. The effect of cultivation parameters on PHA accumulation was studied, including substrate concentration, carbon to nitrogen (C/N) ratio, initial pH-value control and fermentation strategies. For the bacterium, a biomass of 3.8 ± 0.1 g/L, PHA of 1.64 ± 0.1 g/L with PHA productivity of 0.027 g/L.h were obtained under batch cultivation using 100% tuna condensate with a C/N ratio of 88:1 and no control of pH. However, the PHA production was increased 1.3-fold when repeated-batch cultivation was applied. The highest biomass (7.5 ± 0.1 g/L) and PHA (3.8 ± 0.1 g/L) with 0.063 g/L.h of PHA productivity were achieved after the third cycle of repeated-batch cultivation. High chemical oxygen demand (COD) removal efficiency of 70% under the optimal condition was also demonstrated. The polymers generated by C. necator TISTR 1095 were characterised. The size of polymer granules was in the range of 0.7-0.8 μm. The polymer produced in the optimal medium under batch and repeated-batch cultivation was identified as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with a 20 mol% of 3-hydroxyvalerate. The molecular mass (Mn) and polydispersity of the polymer were 2 × 106 Da and 2.5, respectively. The results demonstrated that tuna condensate could be used as a cheap substrate for PHA production on an industrial scale.

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References

  1. 1.

    Nikel PI, Almeida A, Melillo EC, Galvagano MA, Pettinari MJ (2006) New recombinant Escherichia coli strain tailored for the production of poly(3-hydroxybutyrate) from agroindustrial by-products. Appl Environ Microb 72:3949–3954. https://doi.org/10.1128/AEM.00044-06

  2. 2.

    Kamilah H, Tsuge T, Yang TA, Sudesh K (2013) Waste cooking oil as substrate for biosynthesis of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-hydroxyhexanoate): turning waste into a value-added product. Malays J Microbiol 9:51–59. https://doi.org/10.21161/mjm.45012

  3. 3.

    Loo CY, Sudesh K (2007) Polyhydroxyalkanoates: bio-based microbial plastics and their properties. Malays Polym J 2:31–57

  4. 4.

    Yunus AMM, Paeveez GKA, Ho CL (2008) Transgenic plants producing polyhydroxyalkanoates. Asia Pac J Mol Biol Biotechnol 16:1–10

  5. 5.

    Bhubalan K, Chuah JA, Shozui F, Brigham CJ, Taguchi S, Sinskey AJ, Rha C, Sudesh K (2011) Characterization of a highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77:2926–2933. https://doi.org/10.1128/AEM.07997-10

  6. 6.

    Song JH, Che OJ, Mun HC, Sung CY, Woojun P (2008) Polyhydroxyalkanoate (PHA) production using waste vegetable oil by Pseudomonas sp. strain DR2. J Microbiol Biotechnol 18:1408–1415

  7. 7.

    Wang HH, Zhou XR, Liu Q, Chen GQ (2011) Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida. Appl Microbiol Biotechnol 89:1497–1507. https://doi.org/10.1007/s00253-010-2964-x

  8. 8.

    Bugnicourt E, Cinelli P, Lazzeri A, Alvarez V (2014) Polyhydroxyalkanoate (PHA): review of synthesis, characteristics, processing and potential applications in packaging. Express Polym Lett 8:791–808. https://doi.org/10.3144/expresspolymlett.2014.82

  9. 9.

    Chen YJ, Huang YC, Lee CY (2014) Production and characterization of medium-chain-length polyhydroxyalkanoates by Pseudomonas mosselii TO7. J Biosci Bioeng 118:145–152. https://doi.org/10.1016/j.jbiosc.2014.01.012

  10. 10.

    Rodriguez-Contreras A (2019) Recent advances in the use of polyhydroxyalkanoates in biomedicine. Bioengineering 6:82. https://doi.org/10.3390/bioengineering6030082

  11. 11.

    Choi D, Chipman DC, Bents SC, Brown RC (2010) A techno-economic analysis of polyhydroxyalkanoate and hydrogen production from syngas fermentation of gasified biomass. Appl Biochem Biotechnol 160:1032–1046. https://doi.org/10.1007/s12010-009-8560-9

  12. 12.

    Koller M, Marsalek L, de Sousa Dias MM, Braunegg G (2016) Producing microbial polyhydroxyalkanoate (PHA) biopolyesters in a sustainable manner. New Biotechnol 37:24–38. https://doi.org/10.1016/j.nbt.2016.05.001

  13. 13.

    Lee SH, Kim JH, Mishra D, Ni YY, Rhee YH (2011) Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid. Bioresour Technol 102:6159–6166. https://doi.org/10.1016/j.biortech.2011.03.025

  14. 14.

    Kang DK, Lee CR, Lee SH, Bae JH, Park YK, Rhee YH, Sung BH, Sohn JH (2017) Production of polyhydroxyalkanoates from sludge palm oil using Pseudomonas putida S12. J Microbiol Biotechnol 27:990–994. https://doi.org/10.4014/jmb.1612.12031

  15. 15.

    Akaraonye E, Keshavars T, Roy I (2010) Production of polyhydroxyalkanoates: the future green materials of choice. J Chem Technol Biotechnol 85:732–743. https://doi.org/10.1002/jctb.2392

  16. 16.

    Hass R, Jin B, Zept FT (2008) Production of poly(3-hydroxybutyrate) from waste potato starch. Biosci Biotechnol Biochem 72:253–256. https://doi.org/10.1271/bbb.70503

  17. 17.

    Cavalheiro JMBT, Almeida MCMD d, Grandfils C, Fonseca MMRd (2009) Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochem 44:509–515. https://doi.org/10.1016/j.procbio.2009.01.008

  18. 18.

    Tontakulchanchai U, Vaivudh S (2015) Anaerobic co-digestion of tuna factory waste and banana crop residue for biogas production. Int J Renew Energy 10:19–25

  19. 19.

    Wongsakul S, Prasertsen P, Bornscheuer UT, Kittikun AH (2003) Synthesis of 2-monoglycerides by alcoholysis of palm oil using immobilized lipases. Eur J Lipid Sci Tech 105:68–73. https://doi.org/10.1002/ejlt.200390019

  20. 20.

    Sultanbawa Y, Asknes A (2006) Tuna process waste: an unexploited resource. Infofish international 3:37–40

  21. 21.

    Fisheries and Oceans Canada (FOC), Gulf Home. 2003. Fish plant effluents: a workshop on sustainability. Canadian Industry report of fisheries and aquatic sciences. In: Morry CJ, Chadwick M, Courtenay S, Mallet P (eds), p 271

  22. 22.

    Hsu KC, Lu GH, Jao CL (2009) Antioxidative properties of peptides prepared from tuna cooking juice hydrolysates with orientase (Bacillus subtilis). Food Res Int 42:647–652. https://doi.org/10.1016/j.foodres.2009.02.014

  23. 23.

    Kim SK, Mendis E (2006) Bioactive compounds from marine processing byproducts—a review. Food Res Int 33:383–393. https://doi.org/10.1016/j.foodres.2005.10.010

  24. 24.

    Monteiro A, Paquincha D, Martins F, Queiros RP, Saraiva JA, Svarc-Gajic J, Nastic N, Delerue-Matos C, Carvalho AP (2018) Liquid by-products from fish canning industry as sustainable sources of ω3 lipid. J Environ Manag 219:9–17. https://doi.org/10.1016/j.jenvman.2018.04.102

  25. 25.

    Blanco M, Sotelo CG, Chapela MJ, Perez-Martin RI (2007) Towards sustainable and efficient use of fishery resources: present and future trends. Trend Food Sci Technol 18:29–36. https://doi.org/10.1016/j.tifs.2006.07.015

  26. 26.

    Zhao H, Jiang M, Xue S, Xie S,Wu X, Guo L (2011) Fish meal can be completely replaced by soy protein concentrate by increasing feeding frequency in Nile tilapia (Oreochromis niloticus). Department of Aquaculture Nutritional.16:648-655. https://doi.org/10.1111/j.1365-2095.2009.00708.x

  27. 27.

    Gamarro EG, Orawattanameteekul W, Sentina J, Gopal TKS (2013) By-products of tuna processing. Globefish Research Programme, FAO 112:1–48

  28. 28.

    Prasertsan P, Prasertsan S, H-Kitikun A (2009) Recycling of agro-industrial wastes through cleaner technology. In: Doelle HW, Rokem S, Berovic M (eds) BIOTECHNOLOGY volume X: fundamentals in biotechnology. Eolss Publishers Co. Ltd., Oxford

  29. 29.

    Kongkum R, Maneerat S, H-Kittikun A (2017) Bacteriocin production by Enterococcus faecalis TS9S17 in MRS medium with tuna condensate as a nitrogen source and its characteristics. Walailak J Sci Technol 14:941–952 http://orcid.org/0000-0003-3634-8853

  30. 30.

    Olsen RL, Toppe J, Karunasagar I (2014) Challenges and realistic opportunities in the use of by-products from processing of fish and shellfish. Trends Food Sci Technol 36:144–151. https://doi.org/10.1016/j.tifs.2014.01.007

  31. 31.

    Alonso L, Cuesta EP, Gilliland SE (2003) Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. J Dairy Sci 86:1941–1946. https://doi.org/10.3168/jds.S0022-0302(03)73781-3

  32. 32.

    AOAC International (ed) (2016) Official methods of analysis of AOAC international, 20th edn AOAC International

  33. 33.

    Dubber D, Gray NF (2010) Replacement of chemical oxygen demand (COD) with total organic carbon (TOC) for monitoring wastewater treatment performance to minimize disposal of toxic analytical waste. J Environ Sci Heal Part A 45:1595–1600. https://doi.org/10.1080/10934529.2010.506116

  34. 34.

    APHA, AWWA, WPCF (1985) Standard methods for the examination of water and wastewater. 16th ed. American Public Health Association, Washington, D.C.

  35. 35.

    Kerven G (1980) Applications of atomic absorption spectroscopy to the analysis of biological materials. University of Queensland, Department of Agriculture

  36. 36.

    Prasertsan P, Choorit W, Suwano S (1993) Optimization for growth of Rhodocyclus gelatinosus in seafood processing effluents. World J Microb Biot 9:593–596

  37. 37.

    Gopinath KP, Kathiravan MN, Srinivasan R, Sankaranarayanan S (2011) Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant. Bioresour Technol 102:3687–3693. https://doi.org/10.1016/j.biortech.2010.11.072

  38. 38.

    Sangkharak K, Prasertsan P (2013) Municipal wastes treatment and production of polyhydroxyalkanoate by modified two-stage batch reactor. J Polym Environ 21:1009–1015. https://doi.org/10.1007/s10924-013-0597-8

  39. 39.

    Fernandez-Castillo R, Rodriguez-Valera F, Gonzalez-Ramos J, Ruiz-Berraquero F (1986) Accumulation of poly(R-3-hydroxybutyrate) by halobacteria. Appl Environ Microbiol 51:214–216

  40. 40.

    Wong PAL, Cheung MK, Lo WH, Chua H, Yu PHF (2004) Investigation of the effects of types of food waste utilized as carbon source on the molecular weight distributions and thermal properties of polyhydroxy-butyrate produced by two strains of microorganisms. E-polymers 31:1-11. https://doi.org/10.1515/epoly.2004.4.1.324

  41. 41.

    Oliveira FC, Dias ML, Castilho LR, Freire DMG (2007) Characterization of poly(3-hydroxybutyrate) produced by Cupriavidus necator in solid-state fermentation. Bioresour Technol 96:633–638. https://doi.org/10.1016/j.biortech.2006.02.022

  42. 42.

    Sunarno JN, Prasertsan P, Duangsuwan W, Cheirsilp B, Sangkharak K (2019) Biodiesel derived crude glycerol and tuna condensate as an alternative low-cost fermentation medium for ethanol production by Enterobacter aerogenes. Ind Crop Prod 138:111451. https://doi.org/10.1016/j.indcrop.2019.06.014

  43. 43.

    Ribera RG, Monteoliva-Sanchez M, Ramos-Cormenzana A (2001) Production of polyhidroxyalkanoates by Pseudomonas putida KT2442 harboring pSK2665 in wastewater from olive oil mills (alpechín). Electron J Biotechn [online] Cited (12 July 2019) Available from: http://www.ejbiotechnology.info/content/vol4/issue2/full/6/index.html

  44. 44.

    Yu J (2001) Production of PHB from starchy wastewater via organic acids. J Biotechnol 86:105–112. https://doi.org/10.1016/s0168-1656(00)00405-3

  45. 45.

    Du G, Chen J, Yu J, Lun S (2001) Continuous production of poly-3-hydroxybutyrate by Ralstonia eutropha in a two stage culture system. J Biotechnol 88:59–65. https://doi.org/10.1016/S0168-1656(01)00266-8

  46. 46.

    Keunun P, Rakkan T, Yunu T, Paichid N, Prasertsan P, Sangkharak K (2018) The production of polyhydroxybutyrate by two-step fermentation and the application of polyhydroxybutyrate as a novel substrate for a biolubricant. J Polym Environ 26:2459–2466. https://doi.org/10.1007/s10924-017-1140-0

  47. 47.

    Sangyoka S, Poomipuk N, Reungsang A (2012) Optimum conditions for the production of polyhydroxybutyrate from cassava wastewater by the newly isolated Cupriavidus sp. KKU38. Sains Malays 41:1211–1216

  48. 48.

    Ahn J, Jho EH, Nam K (2015) Effect of C/N ratio on polyhydroxyalkanoates (PHA) accumulation by Cupriavidus necator and its implication on the use of rice straw hydrolysates. Environ Eng Res 20:246–253. https://doi.org/10.4491/eer.2015.055

  49. 49.

    Yang YH, Brigham CJ, Budde CF, Boccazzi P, Willis LB, Hassan MA, Yusof ZA, Rha C, Sinskey AJ (2010) Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biot 87:2037–2045. https://doi.org/10.1007/s00253-010-2699-8

  50. 50.

    Baei MS, Najafpour G, Younesi H, Tabandeh F, Eisazadeh H (2009) Poly (3-hydroxybutyrate) synthesis by Cupriavidus necator DSMZ 545 utilizing various carbon sources. World Appl Sci J 7:157–161

  51. 51.

    Madkour MH, Heinrich D, Alghamdi MA, Shabbaj II, Steinbüchel A (2013) PHA recovery from biomass. Biomacromolecules 14:2963–2972. https://doi.org/10.1021/bm4010244

  52. 52.

    Faccin DJL, Martins I, Cardozo NSM, Rech R, Ayub MAZ, Alves TLM, Gambetta R, Secch AR (2009) Optimization of C: N ratio and minimal initial carbon source for poly(3-hydroxybutyrate) production by Bacillus megaterium. J Chem Technol Biotechnol 84:1756–1761. https://doi.org/10.1002/jctb.2240

  53. 53.

    Wang YJ, Hua FL, Tsang YF, Chan SY, Sin SN, Chua H, Yu PHF, Ren NQ (2007) Synthesis of PHAs from waster under various C: N ratios. Bioresour Technol 98:1690–1693. https://doi.org/10.1016/j.biortech.2006.05.039

  54. 54.

    Ma C, Chua H, Yu P, Hong K (2000) Optimal production of polyhydroxyalkanoates in activated sludge biomass. Appl Biochem Biotech 84:981–989. https://doi.org/10.1385/ABAB:84-86:1-9:981

  55. 55.

    Kulpreecha S, Boonruangthavorn A, Meksiriporn B, Thongchul N (2009) Inexpensive fed-batch cultivation for high poly(3-hydroxybutyrate) production by a new isolate of Bacillus megaterium. J Biosci Bioeng 107:240–245. https://doi.org/10.1016/j.jbiosc.2008.10.006

  56. 56.

    Prasertsan P, Jaturapornpipat M, Siripatana C (1997) Utilization and treatment of tuna condensate by photosynthetic bacteria. Pure Appl Chem 69:2439–2445. https://doi.org/10.1351/pac199769112439

  57. 57.

    Aly MM, Albureikan MO, Rabey HE, Kabli SA (2013) Effects of culture conditions on growth and poly-β-hydroxybutyric acid production by Bacillus cereus MM7 isolated from soil samples from Saudi Arabia. Life Sci J 10:1884–1891

  58. 58.

    Gahlawat G, Srivastava AK (2017) Enhancing the production of polyhydroxyalkanoate biopolymer by Azohydromonas Australica using a simple empty and fill bioreactor cultivation strategy. Chem Biochem Eng Q 31:479–485. https://doi.org/10.15255/CABEQ.2017.1148

  59. 59.

    Povolo S, Basaglia M, Fontana F, Morelli A, Casella S (2015) Poly(hydroxyalkanoate) production by Cupriavidus necator from fatty waste can be enhanced by phaZ1 inactivation. Chem Biochem Eng 29:67–74. https://doi.org/10.15255/CABEQ.2014.2248

  60. 60.

    Verlinden RAJ, Hill DJ, Kenward MA, Williams CD, Piotrowska-Seget Z, Radecka IK (2011) Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator. AMB Express 1:11. https://doi.org/10.1186/2191-0855-1-11

  61. 61.

    Povolo S, Toffano P, Basaglia M, Casella S (2010) Polyhydroxyalkanoates production by engineered Cupriavidus necator from waste material containing lactose. Bioresour Technol 101:7902–7907. https://doi.org/10.1016/j.biortech.2010.05.029

  62. 62.

    Poomipuk N, Reungsang A, Plangklang P (2014) Poly-β-hydroxyalkanoates production from cassava starch hydrolysate by Cupriavidus sp. KKU38. Int J Biol Macromol 65:51–64. https://doi.org/10.1016/j.ijbiomac.2014.01.002

  63. 63.

    Taniguchi I, Kagotani K, Kimura Y (2003) Microbial production of poly(hydroxyalkanoate)s from waste edible oils. Green Chem 5:545–548. https://doi.org/10.1039/B304800B

  64. 64.

    Cruz MV, Paiva A, Lisboa P, Freitas F, Alves VD, Simões P, Barreiros S, Reis MAM (2014) Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology. Bioresour Technol 157:360–363. https://doi.org/10.1016/j.biortech.2014.02.013

  65. 65.

    Koller M, Bona R, Hermann C, Horvat P, Martinz J, Neto J, Pereira L, Varila P, Braunegg G (2005) Biotechnological production of poly(3-hydroxybutyrate) with Wautersia eutropha by application of green grass juice and silage juice as additional complex substrate. Biocatal Biotransfor 23:329–337. https://doi.org/10.1080/10242420500292252

  66. 66.

    Hass C, Steinwandter V, Diaz De Apodaca E, Maestro Madurga B, Smerilli M, Dietrich T, Neureiter M (2015) Production of PHB from chicory roots-comparison of three Cupriavidus necator strains. Chem Biochem Eng Q 29:99–112. https://doi.org/10.15255/CABEQ.2014.2250

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Sangkharak, K., Paichid, N., Yunu, T. et al. Utilisation of tuna condensate waste from the canning industry as a novel substrate for polyhydroxyalkanoate production. Biomass Conv. Bioref. (2020) doi:10.1007/s13399-019-00581-4

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Keywords

  • Cupriavidus necator
  • 3-Hydroxybutyrate
  • 3-Hydroxyvalerate
  • Repeated-batch cultivation
  • Tuna condensate