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Alkaline hydrogen peroxide-pretreated sugarcane tops for bioethanol production—a process optimization study

  • Subramaniapillai NijuEmail author
  • Thangavelu Nishanthini
  • Muthusamy Balajii
Original Article
  • 26 Downloads

Abstract

In the present work, sugarcane tops (SCT), an agricultural residue, were subjected to alkaline hydrogen peroxide (AHP) subsequently followed by acid hydrolysis for the maximum release of fermentable sugars for bioethanol production. The process parameters that affect the AHP pretreatment efficiency include AHP concentration (%, v/v), SCT loading (%, w/v), pretreatment time (h), pretreatment temperature (°C), and agitation speed (rpm) were studied for effective delignification. Furthermore, central composite design (CCD) based on response surface methodology (RSM) was applied for optimizing various process parameters of acid hydrolysis such as sulfuric acid concentration (%, v/v), reaction time (min), and reaction temperature (°C). The optimal hydrolysis conditions yielded 6.911 g/L of xylose and 4.106 g/L of glucose. The hydrolyzate containing fermentable sugars was fermented using Saccharomyces cerevisiae, and about 9.9 vol% ethanol was obtained at the end of 24 h fermentation. The native, pretreated, and hydrolyzed SCT were also characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) techniques.

Graphical abstract

Keywords

Sugarcane tops Pretreatment Acid hydrolysis Bioethanol 

Notes

References

  1. 1.
    Karim RA, Hussain AS, Mohd Zain A (2014) Production of bioethanol from empty fruit bunches cellulosic biomass and Avicel PH-101 cellulose. Biomass Convers Biorefinery 4:333–340.  https://doi.org/10.1007/s13399-014-0117-7 CrossRefGoogle Scholar
  2. 2.
    Bhagwat S, Girma AD, Kumar A (2016) Statistical optimization of enzymatic saccharification of acid pretreated Parthenium hysterophorus biomass using response surface methodology. Biofuels 1–9.  https://doi.org/10.1080/17597269.2016.1163214 CrossRefGoogle Scholar
  3. 3.
    Sherpa KC, Ghangrekar MM, Banerjee R (2017) Optimization of saccharification of enzymatically pretreated sugarcane tops by response surface methodology for ethanol production. Biofuels 7269:1–8.  https://doi.org/10.1080/17597269.2017.1409058 CrossRefGoogle Scholar
  4. 4.
    Champagne P (2008) Bioethanol from agricultural waste residues. Environ Prog 27:51–57.  https://doi.org/10.1002/ep.10242 CrossRefGoogle Scholar
  5. 5.
    Das SP, Ravindran R, Ghosh A et al (2014) Efficient pretreatment for bioethanol production from water hyacinth (Eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery. Environ Prog Sustain Energy 33:1396–1404.  https://doi.org/10.1002/ep.11885 CrossRefGoogle Scholar
  6. 6.
    Sivamani S, Baskar R (2015) Optimization of bioethanol production from cassava peel using statistical experimental design. Environ Prog Sustain Energy 34:567–574.  https://doi.org/10.1002/ep.11984 CrossRefGoogle Scholar
  7. 7.
    Jain RK, Ghosh D, Agrawal D, Suman SK, Pandey D, Vadde VT, Dixit AK, Adhikari DK, Dasgupta D (2015) Ethanol production from rice straw using thermotolerant Kluyveromyces sp. IIPE453. Biomass Convers Biorefinery 5:331–337.  https://doi.org/10.1007/s13399-014-0143-5 CrossRefGoogle Scholar
  8. 8.
    Bezerra TL, Ragauskas AJ (2016) A review of sugarcane bagasse for second-generation bioethanol and biopower production. Biofuels Bioprod Biorefin 10:634–647.  https://doi.org/10.1002/bbb CrossRefGoogle Scholar
  9. 9.
    FAO (2018) Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#data/QC. Accessed 31 Jul 2018
  10. 10.
    Sindhu R, Kuttiraja M, Binod P, Janu KU, Sukumaran RK, Pandey A (2011) Dilute acid pretreatment and enzymatic saccharification of sugarcane tops for bioethanol production. Bioresour Technol 102:10915–10921.  https://doi.org/10.1016/j.biortech.2011.09.066 CrossRefGoogle Scholar
  11. 11.
    Pandey A, Biswas S, Sukumaran R, Kaushik N (2009) Study on availability of Indian biomass resources for exploitation: a report based on a nation-wise survey. TIFAC, New DelhiGoogle Scholar
  12. 12.
    Sherpa KC, Ghangrekar MM, Banerjee R (2018) A green and sustainable approach on statistical optimization of laccase mediated delignification of sugarcane tops for enhanced saccharification. J Environ Manag 217:700–709.  https://doi.org/10.1016/j.jenvman.2018.04.008 CrossRefGoogle Scholar
  13. 13.
    Raghavi S, Sindhu R, Binod P, Gnansounou E, Pandey A (2016) Development of a novel sequential pretreatment strategy for the production of bioethanol from sugarcane trash. Bioresour Technol 199:202–210.  https://doi.org/10.1016/j.biortech.2015.08.062 CrossRefGoogle Scholar
  14. 14.
    Yuan Z, Wen Y, Li G (2018) Production of bioethanol and value added compounds from wheat straw through combined alkaline/alkaline-peroxide pretreatment. Bioresour Technol 259:228–236.  https://doi.org/10.1016/j.biortech.2018.03.044 CrossRefGoogle Scholar
  15. 15.
    Martínez-Patiño JC, Ruiz E, Romero I, Cara C, López-Linares JC, Castro E (2017) Combined acid/alkaline-peroxide pretreatment of olive tree biomass for bioethanol production. Bioresour Technol 239:326–335.  https://doi.org/10.1016/j.biortech.2017.04.102 CrossRefGoogle Scholar
  16. 16.
    Morando LEN, Gómez CXD, Zamora LL, Uscanga MGA (2014) Statistical optimization of alkaline hydrogen peroxide pretreatment of sugarcane bagasse for enzymatic saccharification with Tween 80 using response surface methodology. Biomass Convers Biorefinery 4:15–23.  https://doi.org/10.1007/s13399-013-0091-5 CrossRefGoogle Scholar
  17. 17.
    Verardi A, Blasi A, Marino T et al (2018) Effect of steam-pretreatment combined with hydrogen peroxide on lignocellulosic agricultural wastes for bioethanol production: analysis of derived sugars and other by-products. J Energy Chem 27:535–543.  https://doi.org/10.1016/j.jechem.2017.11.007 CrossRefGoogle Scholar
  18. 18.
    Akhtar N, Gupta K, Goyal D, Goyal A (2016) Recent advances in pretreatment technologies for efficient hydrolysis of lignocellulosic biomass. Environ Prog Sustain Energy 35:489–511.  https://doi.org/10.1002/ep.12257 CrossRefGoogle Scholar
  19. 19.
    Srinorakutara T, Suttikul S, Butivate E et al (2014) Optimization on pretreatment and enzymatic hydrolysis of sugarcane trash for ethanol production. J Food Sci Eng 4:148–154Google Scholar
  20. 20.
    Sindhu R, Kuttiraja M, Binod P et al (2014) Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology. Renew Energy 62:362–368.  https://doi.org/10.1016/j.renene.2013.07.041 CrossRefGoogle Scholar
  21. 21.
    Althuri A, Banerjee R (2017) Separate and simultaneous saccharification and fermentation of a pretreated mixture of lignocellulosic biomass for ethanol production. Biofuels 7269:1–12.  https://doi.org/10.1080/17597269.2017.1409059 CrossRefGoogle Scholar
  22. 22.
    Maurya DP, Vats S, Rai S, Negi S (2013) Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface methodology for biofuel. Indian J Exp Biol 51:992–996Google Scholar
  23. 23.
    Sindhu R, Kuttiraja M, Binod P, Preeti VE, Sandhya SV, Vani S, Sukumaran RK, Pandey A (2012) Surfactant-assisted acid pretreatment of sugarcane tops for bioethanol production. Appl Biochem Biotechnol 167:1513–1526.  https://doi.org/10.1007/s12010-012-9557-3 CrossRefGoogle Scholar
  24. 24.
    Sindhu R, Kuttiraja M, Elizabeth Preeti V et al (2013) A novel surfactant-assisted ultrasound pretreatment of sugarcane tops for improved enzymatic release of sugars. Bioresour Technol 135:67–72.  https://doi.org/10.1016/j.biortech.2012.09.050 CrossRefGoogle Scholar
  25. 25.
    Abdollahi M, Hosseini A (2014) Hydrogen Peroxide. In: Encyclopedia of toxicology, 3rd edn. Elsevier, pp 967–970.  https://doi.org/10.1016/B978-0-12-386454-3.00736-3 CrossRefGoogle Scholar
  26. 26.
    Gould JM (1985) Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification. Biotechnol Bioeng 26:46–52.  https://doi.org/10.1002/bit.260270303 CrossRefGoogle Scholar
  27. 27.
    Michalska K, Ledakowicz S (2016) Alkaline peroxide pretreatment for an effective biomass degradation. In: Mussatto SI (ed) Biomass fractionation technologies for a lignocellulosic feedstock based biorefinery. Elsevier, pp 483–498.  https://doi.org/10.1016/B978-0-12-802323-5.00021-9 CrossRefGoogle Scholar
  28. 28.
    Taherzadeh MJ, Karimi K (2008) Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review. Int J Mol Sci 9:1621–1651.  https://doi.org/10.3390/ijms9091621 CrossRefGoogle Scholar
  29. 29.
    Karagöz P, Rocha IV, Özkan M, Angelidaki I (2012) Alkaline peroxide pretreatment of rapeseed straw for enhancing bioethanol production by same vessel saccharification and co-fermentation. Bioresour Technol 104:349–357.  https://doi.org/10.1016/j.biortech.2011.10.075 CrossRefGoogle Scholar
  30. 30.
    Ramadoss G, Muthukumar K (2016) Mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production. Ultrason Sonochem 28:207–217.  https://doi.org/10.1016/j.ultsonch.2015.07.006 CrossRefGoogle Scholar
  31. 31.
    Rabelo SC, Filho RM, Costa AC (2008) A comparison between lime and alkaline hydrogen peroxide pretreatments of sugarcane bagasse for ethanol production. Appl Biochem Biotechnol 148:45–58.  https://doi.org/10.1007/s12010-008-8200-9 CrossRefGoogle Scholar
  32. 32.
    Rabelo SC, Andrade RR, Maciel Filho R, Costa AC (2014) Alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis and fermentation of sugarcane bagasse to ethanol. Fuel 136:349–357.  https://doi.org/10.1016/j.fuel.2014.07.033 CrossRefGoogle Scholar
  33. 33.
    Saha BC, Cotta MA (2007) Enzymatic saccharification and fermentation of alkaline peroxide pretreated rice hulls to ethanol. Enzym Microb Technol 41:528–532.  https://doi.org/10.1016/j.enzmictec.2007.04.006 CrossRefGoogle Scholar
  34. 34.
    Ayeni AO, Daramola MO, Sekoai PT, Adeeyo O, Garba MJ, Awosusi AA (2018) Statistical modelling and optimization of alkaline peroxide oxidation pretreatment process on rice husk cellulosic biomass to enhance enzymatic convertibility and fermentation to ethanol. Cellulose 25:2487–2504.  https://doi.org/10.1007/s10570-018-1714-6 CrossRefGoogle Scholar
  35. 35.
    Qiu J, Ma L, Shen F, Yang G, Zhang Y, Deng S, Zhang J, Zeng Y, Hu Y (2017) Pretreating wheat straw by phosphoric acid plus hydrogen peroxide for enzymatic saccharification and ethanol production at high solid loading. Bioresour Technol 238:174–181.  https://doi.org/10.1016/j.biortech.2017.04.040 CrossRefGoogle Scholar
  36. 36.
    Cao W, Sun C, Qiu J, Li X, Liu R, Zhang L (2016) Pretreatment of sweet sorghum bagasse by alkaline hydrogen peroxide for enhancing ethanol production. Korean J Chem Eng 33:873–879.  https://doi.org/10.1007/s11814-015-0217-5 CrossRefGoogle Scholar
  37. 37.
    Correia JA d C, Júnior JEM, Gonçalves LRB et al (2013) Alkaline hydrogen peroxide pretreatment of cashew apple bagasse for ethanol production: study of parameters. Bioresour Technol 139:249–256.  https://doi.org/10.1016/j.biortech.2013.03.153 CrossRefGoogle Scholar
  38. 38.
    Li Y, Cui J, Zhang G et al (2016) Optimization study on the hydrogen peroxide pretreatment and production of bioethanol from seaweed Ulva prolifera biomass. Bioresour Technol.  https://doi.org/10.1016/j.biortech.2016.04.090 CrossRefGoogle Scholar
  39. 39.
    Li K, Qin JC, Liu CG, Bai FW (2016) Optimization of pretreatment, enzymatic hydrolysis and fermentation for more efficient ethanol production by Jerusalem artichoke stalk. Bioresour Technol 221:188–194.  https://doi.org/10.1016/j.biortech.2016.09.021 CrossRefGoogle Scholar
  40. 40.
    Yuan Z, Wen Y, Kapu NS (2018) Ethanol production from bamboo using mild alkaline pre-extraction followed by alkaline hydrogen peroxide pretreatment. Bioresour Technol 247:242–249.  https://doi.org/10.1016/j.biortech.2017.09.080 CrossRefGoogle Scholar
  41. 41.
    Alvarez-Vasco C, Zhang X (2017) Alkaline hydrogen peroxide (AHP) pretreatment of softwood: enhanced enzymatic hydrolysability at low peroxide loadings. Biomass Bioenergy 96:96–102.  https://doi.org/10.1016/j.biombioe.2016.11.005 CrossRefGoogle Scholar
  42. 42.
    Zhang L, You T, Zhang L, Yang H, Xu F (2014) Enhanced fermentability of poplar by combination of alkaline peroxide pretreatment and semi-simultaneous saccharification and fermentation. Bioresour Technol 164:292–298.  https://doi.org/10.1016/j.biortech.2014.04.075 CrossRefGoogle Scholar
  43. 43.
    Li M, Foster C, Kelkar S et al (2012) Structural characterization of alkaline hydrogen peroxide pretreated grasses exhibiting diverse lignin phenotypes. Biotechnol Biofuels 5:1–15.  https://doi.org/10.1186/1754-6834-5-38 CrossRefGoogle Scholar
  44. 44.
    Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428.  https://doi.org/10.1021/ac60147a030 CrossRefGoogle Scholar
  45. 45.
    Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794.  https://doi.org/10.1177/004051755902901003 CrossRefGoogle Scholar
  46. 46.
    Ramadoss G, Muthukumar K (2016) Ultrasound assisted metal chloride treatment of sugarcane bagasse for bioethanol production. Renew Energy 99:1092–1102.  https://doi.org/10.1016/j.renene.2016.08.003 CrossRefGoogle Scholar
  47. 47.
    Parker FS (1971) Applications of infrared spectroscopy in biochemistry, biology, and medicine. Plenum Press, New York https://link.springer.com/book/10.1007/978-1-4684-1872-9. Accessed 30 July 2018CrossRefGoogle Scholar
  48. 48.
    Ramadoss G, Muthukumar K (2015) Influence of dual salt on the pretreatment of sugarcane bagasse with hydrogen peroxide for bioethanol production. Chem Eng J 260:178–187.  https://doi.org/10.1016/j.cej.2014.08.006 CrossRefGoogle Scholar
  49. 49.
    Su Y, Du R, Guo H et al (2015) Fractional pretreatment of lignocellulose by alkaline hydrogen peroxide: characterization of its major components. Food Bioprod Process 94:322–330.  https://doi.org/10.1016/j.fbp.2014.04.001 CrossRefGoogle Scholar
  50. 50.
    Moodley P, Kana EBG (2017) Microwave-assisted inorganic salt pretreatment of sugarcane leaf waste: effect on physiochemical structure and enzymatic saccharification. Bioresour Technol 235:35–42.  https://doi.org/10.1016/j.biortech.2017.03.031 CrossRefGoogle Scholar
  51. 51.
    Karatzos SK, Edye LA, Doherty WOS (2012) Sugarcane bagasse pretreatment using three imidazolium-based ionic liquids; mass balances and enzyme kinetics. Biotechnol Biofuels 5:1–12.  https://doi.org/10.1186/1754-6834-5-62 CrossRefGoogle Scholar
  52. 52.
    Hinterstoisser B, Akerholm M, Salmén L (2001) Effect of fiber orientation in dynamic FTIR study on native cellulose. Carbohydr Res 334:27–37.  https://doi.org/10.1016/S0008-6215(01)00167-7 CrossRefGoogle Scholar
  53. 53.
    Coates J (2000) Interpretation of infrared spectra, a practical approach. John Wiley & Sons Ltd, Chichester.  https://doi.org/10.1002/9780470027318.a5606 CrossRefGoogle Scholar
  54. 54.
    Kamalini A, Muthusamy S, Ramapriya R et al (2018) Optimization of sugar recovery efficiency using microwave assisted alkaline pretreatment of cassava stem using response surface methodology and its structural characterization. J Mol Liq 254:55–63.  https://doi.org/10.1016/j.molliq.2018.01.091 CrossRefGoogle Scholar
  55. 55.
    Li J, Lu M, Guo X, Zhang H, Li Y, Han L (2018) Insights into the improvement of alkaline hydrogen peroxide (AHP) pretreatment on the enzymatic hydrolysis of corn stover: chemical and microstructural analyses. Bioresour Technol 265:1–7.  https://doi.org/10.1016/j.biortech.2018.05.082 CrossRefGoogle Scholar
  56. 56.
    Wang Q, Hu J, Shen F, Mei Z, Yang G, Zhang Y, Hu Y, Zhang J, Deng S (2016) Pretreating wheat straw by the concentrated phosphoric acid plus hydrogen peroxide (PHP): investigations on pretreatment conditions and structure changes. Bioresour Technol 199:245–257.  https://doi.org/10.1016/j.biortech.2015.07.112 CrossRefGoogle Scholar
  57. 57.
    Moodley P, Gueguim Kana EB (2018) Comparative study of three optimized acid-based pretreatments for sugar recovery from sugarcane leaf waste: a sustainable feedstock for biohydrogen production. Eng Sci Technol Int J 21:107–116.  https://doi.org/10.1016/j.jestch.2017.11.010 CrossRefGoogle Scholar
  58. 58.
    Lange F, Queiroz P, Henrique P et al (2018) Acid , alkali and peroxide pretreatments increase the cellulose accessibility and glucose yield of banana pseudostem. Ind Crop Prod 115:62–68.  https://doi.org/10.1016/j.indcrop.2018.02.024 CrossRefGoogle Scholar
  59. 59.
    Dutra ED, Santos FA, Ribeiro B et al (2017) Alkaline hydrogen peroxide pretreatment of lignocellulosic biomass : status and perspectives. Biomass Convers Biorefinery 8:225–234.  https://doi.org/10.1007/s13399-017-0277-3 CrossRefGoogle Scholar
  60. 60.
    Gould JM (1985) Enhanced polysaccharide recovery from agricultural residues and perennial grasses treated with alkaline hydrogen peroxide. Biotechnol Bioeng 27:893–896.  https://doi.org/10.1002/bit.260270622 CrossRefGoogle Scholar
  61. 61.
    Sun RC, Fang JM, Tomkinson J (2000) Delignification of rye straw using hydrogen peroxide. Ind Crop Prod 12:71–83.  https://doi.org/10.1016/S0926-6690(00)00039-X CrossRefGoogle Scholar
  62. 62.
    Hideno A (2017) Short-time alkaline peroxide pretreatment for rapid pulping and efficient enzymatic hydrolysis of rice straw. Bioresour Technol 230:140–142.  https://doi.org/10.1016/j.biortech.2017.01.058 CrossRefGoogle Scholar
  63. 63.
    Moodley P, Kana EBG (2015) Optimization of xylose and glucose production from sugarcane leaves (Saccharum officinarum) using hybrid pretreatment techniques and assessment for hydrogen generation at semi-pilot scale. Int J Hydrog Energy 40:3859–3867.  https://doi.org/10.1016/j.ijhydene.2015.01.087 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyPSG College of TechnologyCoimbatoreIndia

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