Journal of NeuroVirology

, Volume 25, Issue 1, pp 50–56 | Cite as

Multimodal techniques failed to detect cytomegalovirus in human glioblastoma samples

  • Marie-Pierre Loit
  • Homa Adle-Biassette
  • Schahrazed Bouazza
  • Marie-Christine Mazeron
  • Philippe Manivet
  • Jacqueline Lehmann-Che
  • Natacha Teissier
  • Emmanuel MandonnetEmail author
  • Jean-Michel Molina


The role of the human cytomegalovirus (HCMV) in gliomagenesis is largely debated. Contradictory data exist regarding the sensitivity and specificity of HCMV detection techniques, including immunohistochemistry (IHC), in situ hybridization (ISH), and RNA and DNA sequencing. The aim of this study is to detect HCMV in glioblastoma (GBM) tumor samples using IHC, ISH, and real-time PCR (qPCR), as well as to correlate the findings with serological status and HCMV DNA load in blood. Forty-seven patients with histopathological diagnosis of GBM and HCMV serological status were retrospectively reviewed. HCMV DNA quantification in whole blood was performed in 31 patients. The detection of HCMV in tumor samples was performed using IHC in 42 cases, ISH in 10 cases, and qPCR in 29 cases. All but two patients were taking high steroid doses at the time of biological testing. HCMV seroprevalence was 68%. Active infection with HCMV DNA detected in blood was diagnosed in 6 out of 21 (28%) seropositive patients. HCMV was not detected in GBM samples using IHC or ISH, while qPCR was positive in one case (also positive for blood HCMV DNA). These data do not support a crucial role of HCMV in GBM tumorigenesis. HCMV might be reactivated in GBM patients, due to steroid treatment.


Glioblastoma Human cytomegalovirus Steroids 



Authors thank Diana Folia and Dr. Darren Russell for their careful English editing of the manuscript.

Compliance with ethical standards

Written consent for use of molecular analysis for research purpose was obtained and recorded in each patient’s medical file. This study was approved by the local Ethics Committee of the Neuroscience division of Lariboisière Hospital.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Journal of NeuroVirology, Inc. 2018

Authors and Affiliations

  • Marie-Pierre Loit
    • 1
    • 2
  • Homa Adle-Biassette
    • 2
    • 3
    • 4
    • 5
  • Schahrazed Bouazza
    • 1
  • Marie-Christine Mazeron
    • 2
    • 6
  • Philippe Manivet
    • 2
    • 5
    • 7
  • Jacqueline Lehmann-Che
    • 2
    • 8
  • Natacha Teissier
    • 2
    • 4
  • Emmanuel Mandonnet
    • 1
    • 2
    • 9
    Email author
  • Jean-Michel Molina
    • 2
    • 10
    • 11
  1. 1.Department of NeurosurgeryLariboisière HospitalParisFrance
  2. 2.Paris 7 Diderot UniversityParisFrance
  3. 3.Department of NeuropathologyLariboisière HospitalParisFrance
  4. 4.INSERM UMR 1141ParisFrance
  5. 5.Biobank BB-0033-00064ParisFrance
  6. 6.Microbiology DepartmentSaint-Louis HospitalParisFrance
  7. 7.Department of BiochemistryLariboisière HospitalParisFrance
  8. 8.Department of Molecular BiologySaint-Louis HospitalParisFrance
  9. 9.Frontlab, INSERM, ICMParisFrance
  10. 10.Department of Infectious DiseasesSaint-Louis HospitalParisFrance
  11. 11.INSERM UMR-S-941ParisFrance

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