Journal of NeuroVirology

, Volume 17, Issue 4, pp 303–313

Polyomavirus JC reactivation and noncoding control region sequence analysis in pediatric Crohn's disease patients treated with infliximab

  • Anna Bellizzi
  • Elena Anzivino
  • Federica Ferrari
  • Giovanni Di Nardo
  • Maria Teresa Colosimo
  • Daniela Fioriti
  • Monica Mischitelli
  • Fernanda Chiarini
  • Salvatore Cucchiara
  • Valeria Pietropaolo
Article

DOI: 10.1007/s13365-011-0036-3

Cite this article as:
Bellizzi, A., Anzivino, E., Ferrari, F. et al. J. Neurovirol. (2011) 17: 303. doi:10.1007/s13365-011-0036-3

Abstract

The recent introduction of monoclonal antibodies in Crohn's disease (CD) management has been associated with the development of serious complications, such as the progressive multifocal leukoencephalopathy (PML), caused by JC polyomavirus (JCV) reactivation. Therefore, the aims of our study have been the investigation of the possible JCV reactivation in pediatric CD patients treated or not with infliximab, performing quantitative PCR in urine, plasma, and intestinal biopsies at the time of recruitment (t0) and every 4 months in 1 year of follow-up (t1, t2, and t3), and the analysis of the JCV noncoding control region (NCCR) to detect cellular transcription factors binding site mutations. Results obtained showed that, in urine and ileal specimens, JCV load significantly increased in infliximab-treated patients after 1 year of treatment (t3), while viremia was significantly higher at t1. JCV NCCR sequence analysis showed a structure similar to CY archetype in 65/80 analyzed sequences, but the remaining 15/80, obtained exclusively from plasma and biopsies, evidenced a CY NCCR organization with two recurrent nucleotide changes, the 37-T to G transversion in box A Spi-B binding site and the 217-G to A transition in box F, and a box D deletion. These rearrangements were always found at t3 within seven infliximab-treated CD patients, who presented a very severe disease at t0. We can conclude that our rearranged NCCR sequences could be considered a marker of JCV virulence during mAb treatment, although none of our examined patients developed PML, and further studies on a larger cohort of patients should be performed.

Keywords

Crohn's disease Infliximab Human polyomavirus JC Noncoding control region Spi-B PML 

Copyright information

© Journal of NeuroVirology, Inc. 2011

Authors and Affiliations

  • Anna Bellizzi
    • 1
  • Elena Anzivino
    • 1
  • Federica Ferrari
    • 2
  • Giovanni Di Nardo
    • 2
  • Maria Teresa Colosimo
    • 1
  • Daniela Fioriti
    • 3
  • Monica Mischitelli
    • 1
  • Fernanda Chiarini
    • 1
  • Salvatore Cucchiara
    • 2
  • Valeria Pietropaolo
    • 1
    • 4
  1. 1.Department of Health Sciences and Infectious Diseases“Sapienza” UniversityRomeItaly
  2. 2.Department of Pediatrics“Sapienza” UniversityRomeItaly
  3. 3.National Institute for Infectious Diseases “Lazzaro Spallanzani”RomeItaly
  4. 4.Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and TechnologyTemple UniversityPhiladelphiaUSA

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