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Rapid Differentiation of Asian and American Ginseng by Differential Ion Mobility Spectrometry-Tandem Mass Spectrometry Using Stepwise Modulation of Gas Modifier Concentration

  • Ri Wu
  • Xiangfeng ChenEmail author
  • Wei-Jing Wu
  • Ze Wang
  • Y.-L. Elaine Wong
  • Y.-L. Winnie Hung
  • H.-T. Wong
  • Minli Yang
  • Feng Zhang
  • T.-W. Dominic ChanEmail author
Research Article

Abstract

This study reports a rapid and robust method for the differentiation of Asian and American ginseng samples based on differential ion mobility spectrometry-tandem mass spectrometry (DMS-MS/MS). Groups of bioactive ginsenoside/pseudo-ginsenoside isomers, including Rf/Rg1/F11, Rb2/Rb3/Rc, and Rd/Re, in the ginseng extracts were sequentially separated using DMS with stepwise changes in the gas modifier concentration prior to MS analysis. The identities of the spatially separated ginsenoside/pseudo-ginsenoside isomers were confirmed by their characteristic compensation voltages at specific modifier loading and MS/MS product ions. As expected, Asian ginseng samples contained some Rf and an insignificant amount of F11, whereas American ginseng samples had a high level of F11 but no Rf. The origin of the whole and sliced ginseng could further be confirmed using the quantitative ratios of three sets of ginsenoside markers, namely, Rg1/Re, Rb1/Rg1, and Rb2/Rc. Based on our results, new benchmark ratios of Rg1/Re < 0.15, Rb1/Rg1 > 2.15, and Rb2/Rc < 0.26 were proposed for American ginseng (as opposed to Asian ginseng).

Keywords

Differential ion mobility spectrometry Tandem mass spectrometry Collision-induced dissociation Isomeric ginsenosides 

Notes

Acknowledgements

This work was supported by The National Key Research and Development Program of China (2018YFC1603500), Research Grant Council of the Hong Kong Special Administrative Region (Research Grant Direct Allocation, Ref. 3132667 and 4053152), Key R&D Program of Shandong Province (2019GSF111009, 2019GSF111001), and Natural Science Foundation of Shandong Province (ZR2017MB011).

Compliance with Ethical Standards

Conflict of Interests

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Supplementary material

13361_2019_2317_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1234 kb)

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Copyright information

© American Society for Mass Spectrometry 2019

Authors and Affiliations

  1. 1.Department of ChemistryThe Chinese University of Hong KongShatin, N.T.People’s Republic of China
  2. 2.Shandong Analysis and Test CentreQilu University of Technology (Shandong Academy of Sciences)JinanPeople’s Republic of China
  3. 3.Institute of Food SafetyChinese Academy of Inspection and QuarantineBeijingChina

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