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Validation of Calibration Parameters for Trapped Ion Mobility Spectrometry

  • Cameron N. Naylor
  • Tobias Reinecke
  • Mark E. Ridgeway
  • Melvin A. Park
  • Brian H. ClowersEmail author
Research Article

Abstract

Using contemporary theory for ion mobility spectrometry (IMS), gas-phase ion mobilities within a trapped ion mobility-mass spectrometer (TIMS) are not easily deduced using first principle equations due to non-linear pressure changes and consequently variations in E/N. It is for this reason that prior literature values have traditionally been used for TIMS calibration. Additionally, given that verified mobility standards currently do not exist and the that the exact conditions used to measure reported literature values may not always represent the environment within the TIMS, a direct approach to validating the behavior of the TIMS system is warranted. A calibration procedure is presented where an ambient pressure, ambient temperature, two-gate, printed circuit board drift-tube IMS (PCBIMS) is coupled to the front of a TIMS allowing reduced mobilities to be directly measured on the same instrument as the TIMS. These measured mobilities were used to evaluate the TIMS calibration procedure which correlates reduced mobility and TIMS elution voltages with literature values. When using the measured PCBIMS-reduced mobilities of tetraalkyl ammonium salts and tune mix for TIMS calibration of the alkyltrimethyl ammonium salts, the percent error is less than 1% as compared with using the reported literature K0 values where the percent error approaches 5%. This method provides a way to obtain accurate reference mobilities for ion mobility techniques that require a calibration step (i.e., TIMS and TWAVE).

Keywords

Trapped ion mobility spectrometry Drift-tube ion mobility spectrometry Ion mobility calibration Mass spectrometry 

Notes

Acknowledgements

The authors would like to thank the Department of Chemistry of the Washington State University and Bruker Daltonics for their support. TR would like to acknowledge support from the NSF under award CHE-1506672 and support for CNN was provided by DTRA Basic Research Program (Grant No. HDTRA1-14-1-0023).

Supplementary material

13361_2019_2289_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1895 kb)

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Copyright information

© American Society for Mass Spectrometry 2019

Authors and Affiliations

  1. 1.Department of ChemistryWashington State UniversityPullmanUSA
  2. 2.Bruker Daltonics Inc.BillericaUSA

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