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Journal of The American Society for Mass Spectrometry

, Volume 30, Issue 9, pp 1801–1812 | Cite as

MeV-SIMS TOF Imaging of Organic Tissue with Continuous Primary Beam

  • Boštjan Jenčič
  • Primož Vavpetič
  • Mitja Kelemen
  • Matjaž Vencelj
  • Katarina Vogel-Mikuš
  • Anja Kavčič
  • Primož PeliconEmail author
Research Article

Abstract

MeV-SIMS is an emerging mass spectrometry imaging method, which utilizes fast, heavy ions to desorb secondary molecules. High yields and low fragmentation rates of large molecules, associated with the electronic sputtering process, make it particularly useful in biomedical research, where insight into distribution of organic molecules is needed. Since the implementation of MeV-SIMS in to the micro-beam line at the tandem accelerator of Jožef Stefan Institute, MeV-SIMS provided some valuable observations on the distribution of biomolecules in plant tissue, as discussed by Jenčič et al. (Nucl. Inst. Methods Phys. Res. B. 371, 205–210, 2016; Nucl. Inst. Methods Phys. Res. B. 404, 140–145, 2017). However, limited focusing ability of the chlorine ion beam only allowed imaging at the tissue level. In order to surpass shortcomings of the existing method, we introduced a new approach, where we employ a continuous, low-current primary beam. In this mode, we bombard thin samples with a steady chlorine ion flux of approx. 5000 ions/s. After desorbing molecules, chlorine ions penetrate through the thinly cut sample and trigger the time-of-flight “start” signal on a continuous electron multiplier detector, positioned behind the sample. Such bombardment is more effective than previously used pulsing-beam mode, which demanded several orders of magnitude higher primary ion beam currents. Sub-micrometer focusing of low-current primary ion beam allows imaging of biological tissue on a subcellular scale. Simultaneously, new time-of-flight acquisition approach also improves mass resolution by a factor of 5. Within the article, we compare the performance of both methods and demonstrate the application of continuous mode on biological tissue. We also describe the thin sample preparation protocol, necessary for measurements with low primary ion currents.

Keywords

MeV-SIMS Time-of-flight Electronic sputtering Molecular imaging Imaging mass spectrometry 

Notes

Acknowledgements

The work at the Jožef Stefan Institute was supported by the Slovenian research agency (ARRS) grants J7-9398, N1-0090, P1-0112, I0-0005, P1-0212, and EU H2020 project no. 824096 “RADIATE.”

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Copyright information

© American Society for Mass Spectrometry 2019

Authors and Affiliations

  1. 1.Jožef Stefan InstituteLjubljanaSlovenia
  2. 2.Biotechnical Faculty, Department of BiologyUniversity of LjubljanaLjubljanaSlovenia

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