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Simple Approach for De Novo Structural Identification of Mannose Trisaccharides

  • Hsu Chen Hsu
  • Chia Yen Liew
  • Shih-Pei Huang
  • Shang-Ting Tsai
  • Chi-Kung Ni
Research Article

Abstract

Oligosaccharides have diverse functions in biological systems. However, the structural determination of oligosaccharides remains difficult and has created a bottleneck in carbohydrate research. In this study, a new approach for the de novo structural determination of underivatized oligosaccharides is demonstrated. A low-energy collision-induced dissociation (CID) of sodium ion adducts was used to facilitate the cleavage of desired chemical bonds during the dissociation. The selection of fragments for the subsequent CID was guided using a procedure that we built from the understanding of the saccharide dissociation mechanism. The linkages, anomeric configurations, and branch locations of oligosaccharides were determined by comparing the CID spectra of oligosaccharide with the fragmentation patterns based on the dissociation mechanism and our specially prepared disaccharide CID spectrum database. The usefulness of this method was demonstrated to determine the structures of several mannose trisaccharides. This method can also be applied in the structural determination of oligosaccharides larger than trisaccharides and containing hexose other than mannose if authentic standards are available.

Graphical Abstract

Keywords

Oligosaccharide Structure CID 

Notes

Acknowledgments

This work was financially supported in part by the Ministry of Science and Technology, Taiwan (103-2113-M-001-011-NY3).

Supplementary material

13361_2017_1850_MOESM1_ESM.docx (84 kb)
ESM 1 (DOCX 84 kb)

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Copyright information

© American Society for Mass Spectrometry 2017

Authors and Affiliations

  1. 1.Institute of Atomic and Molecular SciencesAcademia SinicaTaipeiTaiwan
  2. 2.Department of ChemistryNational Taiwan Normal UniversityTaipeiTaiwan
  3. 3.Department of ChemistryNational Tsing Hua UniversityHsinchuTaiwan

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