Multiphoton Dissociation of Electrosprayed MegaDalton-Sized DNA Ions in a Charge-Detection Mass Spectrometer

  • Tristan Doussineau
  • Pierre Paletto
  • Philippe Dugourd
  • Rodolphe Antoine
Research Article


Charge detection mass spectrometry in combination with a linear electrostatic ion trap coupled to a continuous wavelength infrared CO2 laser has been used to study the multiphoton dissociation of DNA macromolecular ions. Samples, with masses ranging from 2.23 to 31.5 MDa, include single strand circular M13mp18, double strand circular M13mp18, and double strand linear LambdaPhage DNA fragments. Their activation energies for unimolecular dissociation were determined. Activation energy values slightly increase as a function of the molecular weight. The most important result is the difference between the fragmentations observed for hybridized double-strands and dimers of single strands.

Graphical Abstract


Charge detection mass-spectrometry Single-molecule Oligonucleotide Gas-phase Radiative dissociation Electrospray ions Nucleic-acids Activation Charge 

Supplementary material

13361_2014_1011_MOESM1_ESM.docx (21 kb)
ESM 1(DOCX 20 kb)


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Copyright information

© American Society for Mass Spectrometry 2014

Authors and Affiliations

  • Tristan Doussineau
    • 1
    • 2
  • Pierre Paletto
    • 1
    • 2
  • Philippe Dugourd
    • 1
    • 2
  • Rodolphe Antoine
    • 1
    • 2
  1. 1.Université Claude Bernard Lyon1-CNRSUniversité de LyonVilleurbanne cedexFrance
  2. 2.Institut lumière matière, UMR5306VilleurbanneFrance

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