Multifaceted Investigation of Metabolites During Nitrogen Fixation in Medicago via High Resolution MALDI-MS Imaging and ESI-MS

  • Erin Gemperline
  • Dhileepkumar Jayaraman
  • Junko Maeda
  • Jean-Michel Ané
  • Lingjun Li
Research Article

Abstract

Legumes have developed the unique ability to establish a symbiotic relationship with soil bacteria known as rhizobia. This interaction results in the formation of root nodules in which rhizobia thrive and reduce atmospheric dinitrogen into plant-usable ammonium through biological nitrogen fixation (BNF). Owing to the availability of genetic information for both of the symbiotic partners, the Medicago truncatulaSinorhizobium meliloti association is an excellent model for examining the BNF process. Although metabolites are important in this symbiotic association, few studies have investigated the array of metabolites that influence this process. Of these studies, most target only a few specific metabolites, the roles of which are either well known or are part of a well-characterized metabolic pathway. Here, we used a multifaceted mass spectrometric (MS) approach to detect and identify the key metabolites that are present during BNF using the Medicago truncatulaSinorhizobium meliloti association as the model system. High mass accuracy and high resolution matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) Orbitrap instruments were used in this study and provide complementary results for more in-depth characterization of the nitrogen-fixation process. We used well-characterized plant and bacterial mutants to highlight differences between the metabolites that are present in functional versus nonfunctional nodules. Our study highlights the benefits of using a combination of mass spectrometric techniques to detect differences in metabolite composition and the distributions of these metabolites in plant biology.

Graphical Abstract

Keywords

Nitrogen fixation Medicago truncatula Metabolites MALDI Orbitrap Mass spectrometry Imaging Q-Exactive 

Supplementary material

13361_2014_1010_MOESM1_ESM.pdf (186 kb)
ESM 1(PDF 186 kb)

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Copyright information

© American Society for Mass Spectrometry 2014

Authors and Affiliations

  • Erin Gemperline
    • 1
  • Dhileepkumar Jayaraman
    • 2
  • Junko Maeda
    • 2
  • Jean-Michel Ané
    • 2
  • Lingjun Li
    • 1
    • 3
  1. 1.Department of ChemistryUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of AgronomyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.School of PharmacyUniversity of Wisconsin-MadisonMadisonUSA

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