Tip-Enhanced Laser Ablation Sample Transfer for Biomolecule Mass Spectrometry
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Atomic force microscope (AFM) tip-enhanced laser ablation was used to transfer molecules from thin films to a suspended silver wire for off-line mass spectrometry using laser desorption ionization (LDI) and matrix-assisted laser desorption ionization (MALDI). An AFM with a 30 nm radius gold-coated silicon tip was used to image the sample and to hold the tip 15 nm from the surface for material removal using a 355 nm Nd:YAG laser. The ablated material was captured on a silver wire that was held 300 μm vertically and 100 μm horizontally from the tip. For the small molecules anthracene and rhodamine 6G, the wire was cut and affixed to a metal target using double-sided conductive tape and analyzed by LDI using a commercial laser desorption time-of-flight mass spectrometer. Approximately 100 fg of material was ablated from each of the 1 μm ablation spots and transferred with approximately 3% efficiency. For larger polypeptide molecules angiotensin II and bovine insulin, the captured material was dissolved in saturated matrix solution and deposited on a target for MALDI analysis.
KeywordsAmbient Laser ablation Atomic force microscope Matrix-assisted laser desorption/ionization
This work was supported by the National Institutes of Health Grant Number R21DA035504. The authors are grateful to R. Shetty and K. Kjoller (Anasys) for helpful discussions.
- 14.Koestler, M., Kirsch, D., Hester, A., Leisner, A., Guenther, S., Spengler, B.: A high-resolution scanning microprobe matrix-assisted laser desorption/ionization ion source for imaging analysis on an ion trap/Fourier transform ion cyclotron resonance mass spectrometer. Rapid Commun. Mass Spectrom. 22, 3275–3285 (2008)CrossRefGoogle Scholar
- 32.Yao, Z.-P.: Characterization of proteins by ambient mass spectrometry. Mass Spectrom. Rev. 31, 437–447 (2012)Google Scholar