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Discrimination of Isobaric Leu/Ile Residues by MALDI In-Source Decay Mass Spectrometry

  • Daiki AsakawaEmail author
  • Nicolas Smargiasso
  • Edwin De Pauw
Application Note

Abstract

MALDI in-source decay (ISD) has been used for top-down sequencing of proteins. The use of the matrix 1,5-diaminonapthalene (1,5-DAN) gave abundant w ions, which are formed from the unimolecular dissociation of z• radical fragments via α cleavage reaction and thus help identify which of the isobaric amino acids, Leu or Ile, is present. The high abundance of w ions in MALDI-ISD with 1,5-DAN results from the low collision rate in the MALDI plume. MALDI-ISD with 1,5-DAN appears to be an useful method for the top-down sequencing of proteins, including discrimination of Leu and Ile near the C-terminal end.

Key words

Initial velocity Side-chain loss 1,5-Diaminonapthalene Negative-ion mode Top-down sequencing 

Notes

Acknowledgment

D.A. acknowledges a research fellowship from the Japan Society for the Promotion of Science for Young Scientists (grant 23-10272). N.S. is a FRS-FNRS logistics collaborator. Walloon Region and FNRS contributed to Mass Spectrometry platform funding.

Supplementary material

13361_2012_523_MOESM1_ESM.doc (944 kb)
ESM 1 (DOC 944 kb)

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Copyright information

© American Society for Mass Spectrometry 2013

Authors and Affiliations

  • Daiki Asakawa
    • 1
    • 2
    Email author
  • Nicolas Smargiasso
    • 1
  • Edwin De Pauw
    • 1
  1. 1.Chemistry Department and GIGA-R, Mass Spectrometry LaboratoryUniversity of LiegeLiegeBelgium
  2. 2.Graduate School of Nano Bioscience, Mass Spectrometry LaboratoryYokohama City UniversityYokohamaJapan

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