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Imaging densitometry of honeydew samples with Densitometric Research Operating Program (DROP)

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Abstract

The amount of excreta from sap-feeding insects is linked to the differences in ingestion volumes, and it provides valuable information such as the presence of feeding stimulants and deterrents in their diets. In this work, we introduce a newly developed program source code named DROP and use it to produce imaging densitometric analyses of the insect excreta. Our method is straightforward, rapid, has high sensitivity, and is sufficiently accurate, and therefore is applicable to many sap-feeding insects. The DROP and the sample images are freely available on the website https://sites.google.com/view/tsuchida-lab-drop.

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References

  1. Auclair JL (1959) Feeding and excretion by the pea aphid, Acyrthosiphon pisum (Harr.) (Homoptera: Aphididdae), reared on different varieties of peas. Entomol Exp Appl 2:279–286. https://doi.org/10.1111/j.1570-7458.1959.tb00441.x

  2. Fukatsu T, Tsuchida T, Nikoh N, Koga R (2001) Spiroplasma symbiont of the pea aphid, Acyrthosiphon pisum (Insecta: Homoptera). Appl Environ Microbiol 67:1284–1291. https://doi.org/10.1128/AEM.67.3.1284-1291.2001

  3. Ihaka R, Gentleman R (1996) R: a language for data analysis and graphics. J Comp Graph Stat 5:299–314. Available via http://www.R-project.org

  4. Kawabe S (1985) Mechanism of varietal resistance to the rice green leafhopper (Nephotettix cincticeps Uhler). JARQ 19:115–124

  5. Mittler TE (1958) Studies on the feeding and nutrition of Tuberolachnus Salignus (Gmelin) (Homoptera, Aphididae) II. The nitrogen and sugar composition of ingested phloem sap and excreted honeydew. J Exp Biol 35:74–84

  6. Molyneux RJ, Campbell BC, Dreyer DL (1990) Honeydew analysis for detecting phloem transport of plant natural products: implications for host-plant resistance to sap-sucking insects. J Chem Ecol 16:1899–1909. https://doi.org/10.1007/BF01020503

  7. Noda H, Sogawa K, Saito T (1973) Amino acids in honeydews of the rice planthoppers and leafhoppers (Homoptera: Delphacidae, Deltocephalidae). Appl Entomol Zool 8:191–197. https://doi.org/10.1303/aez.8.191

  8. Paguia P, Pathak MD, Heinrichs EA (1980) Honeydew excretion measurement techniques for determining differential feeding activity of biotypes of Nilaparvata lugens on rice varieties. J Econ Entomol 73:35–40. https://doi.org/10.1093/jee/73.1.35

  9. Sasaki T, Aoki T, Hayashi H, Ishikawa H (1990) Amino acid composition of the honeydew of symbiotic and aposymbiotic pea aphids Acyrthosiphon pisum. J Insect Physiol 36:35–40. https://doi.org/10.1016/0022-1910(90)90148-9

  10. Sasaki T, Hayashi H, Ishikawa H (1991) Growth and reproduction of the symbiotic and aposymbiotic pea aphids, Acyrthosiphon pisum maintained on artificial diets. J Insect Physiol 10:749–756. https://doi.org/10.1016/0022-1910(91)90109-D

  11. Sogawa K, Pathak MD (1970) Mechanisms of brown planthopper resistance in Mudgo variety of rice (Hemiptera: Delphacidae). Appl Entomol Zool 5:145–158. https://doi.org/10.1303/aez.5.145

  12. Yemm EW, Cocking E (1955) The determination of amino-acids with ninhydrin. Analyst 80:209–214. https://doi.org/10.1039/AN9558000209

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Acknowledgements

We thank T. Ichishi, N. Murakami, K. Tanaka, M. Yoshizaki for their technical assistance; N. Haruyama for the whitefly samples; T. Fukatsu for the useful comments. This study was supported by grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (Research Program on development of innovative technology). A. F. was supported by the Leading Initiative for Excellent Young Researchers (LEADER) Program.

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Correspondence to Tsutomu Tsuchida.

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Tsuchida, T., Wasano, N., Wang, Y. et al. Imaging densitometry of honeydew samples with Densitometric Research Operating Program (DROP). Appl Entomol Zool (2020). https://doi.org/10.1007/s13355-020-00672-z

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Keywords

  • Densitometry
  • Honeydew
  • Aphid
  • Whitefly
  • Image analysis