Laboratory rearing of Lytopylus rufipes (Hymenoptera: Braconidae: Agathidinae), a parasitoid wasp of the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), using apple and a commercially available diet

Applied Entomology and Zoology volume 55, pages271–276(2020)Cite this article

Abstract

Lytopylus rufipes (Nees) (Hymenoptera: Braconidae: Agathidinae) is a potential natural enemy of the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), but there is no established method to rear this wasp continuously. In the laboratory, female wasps can produce both female and male progenies without mating (deuterotokous), but host-infested plants are necessary to trigger oviposition behavior. In this study, immature apples were used because they keep well. Grapholita molesta larvae were transferred to immature apples, and then exposed to L. rufipes females. After parasitization, these apples were transferred to blocks of artificial diet (Silkmate 2M) for further rearing. Using this transitional diet system, L. rufipes females develop in 25.1 ± 1.8 (mean ± SD) days from egg to adult, but male wasps require only 23.8 ± 1.0 days. Furthermore, the longevity of female wasps was 12.2 ± 7.3 (mean ± SD) days, and the parasitism rate was 26.4 (95% Cl: 22.2–30.6). Female wasps can attack host larvae in all instars, but parasitism of first-instar larvae is more successful. Therefore, first-instar larvae of G. molesta are recommended for establishing a L. rufipes colony under laboratory conditions. This system decreases the requirement for plant material and maintains continuous production of L. rufipes.

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References

  1. Allen H (1962) Parasites of the oriental fruit moth in the eastern United States. USDA Tech Bull No. 1265

  2. Allen HW, Yetter WP (1949) Bassus diversus, an oriental fruit moth parasite established in the United States. J Econ Entomol 42:540

    CAS  Google Scholar 

  3. Bailey P (1979) An attempt to control oriental fruit moth, Cydia molesta Busckby mass releases of Macrocentrus ancylivorus Rohwer (Hymenoptera: Braconidae). J Aust Entomol Soc 18:211–212. https://doi.org/10.1111/j.1440-6055.1979.tb00839.x

    Article  Google Scholar 

  4. Brunson MH, Allen HW (1944) Mass liberation of parasites for immediate reduction of oriental fruit moth injury to ripe peaches. J Econ Entomol 37:411–416. https://doi.org/10.1093/jee/37.3.411

    Article  Google Scholar 

  5. Croft P, Copland MJW (1995) The effect of host instar on the size and sex ratio of the endoparasitoid Dacnusa sibirica. Entomol Exp Appl 74:121–124. https://doi.org/10.1111/j.1570-7458.1995.tb01883.x

    Article  Google Scholar 

  6. de Lame FM, Hong J, Shearer PW, Brattsten LB (2001) Sex-related differences in the tolerance of Oriental fruit moth (Grapholita molesta) to organophosphate insecticides. Pest Manag Sci 57:827–832. https://doi.org/10.1002/ps.368

    Article  PubMed  Google Scholar 

  7. Endo K (1966) Studies on the control of oriental fruit moth, Grapholitha molesta Busck. J Jpn Soc Hortic Sci 35:339–344. https://doi.org/10.2503/jjshs.35.339(in Japanese with English summary)

    Article  Google Scholar 

  8. Farahani S, Talebi AA, Rakhshani E, van Achterberg C, Sharkey M (2014) A contribution to the knowledge of Agathidinae (Hymenoptera: Braconidae) from Iran with description of a new species. Biologia 69:228–235. https://doi.org/10.2478/s11756-013-0295-y

    Article  Google Scholar 

  9. Haeussler GJ (1940) Parasites of the oriental fruit moth in Japan and chosen and their introduction into the United States. USDA Tech Bull No.728

  10. Harvey JA, Strand MR (2002) The developmental strategies of endoparasitoid wasps vary with host feeding ecology. Ecology 83:2349–2451. https://doi.org/10.1890/0012-9658(2002)083[2439:TDSOEW]2.0.CO;2

    Article  Google Scholar 

  11. Harvey JA, Strand MR (2003) Sexual size and development time dimorphism in a parasitoid wasp: an exception to the rule. Eur J Entomol 100:485–492. https://doi.org/10.14411/eje.2003.074

    Article  Google Scholar 

  12. Harvey JA, Harvey IF, Thompson DJ (1994) Flexible larval growth allows use of a range of host sizes by a parasitoid wasp. Ecology 75:1420–1428. https://doi.org/10.2307/1937465

    Article  Google Scholar 

  13. Harvey JA, Kadash K, Strand MR (2000) Differences in larval feeding behavior correlate with altered developmental strategies in two parasitic wasps: implications for the size-fitness hypothesis. Oikos 88:621–629. https://doi.org/10.1034/j.1600-0706.2000.880319.x

    Article  Google Scholar 

  14. Idriss GEA, Mohamed SA, Khamis F, Du Plessis H, Ekesi S (2018) Biology and performance of two indigenous larval parasitoids on Tuta absoluta (Lepidoptera: Gelechiidae) in Sudan. Biocontrol Sci Technol 28:614–628. https://doi.org/10.1080/09583157.2018.1477117

    Article  Google Scholar 

  15. Kanga LHB, Pree DJ, van Lier JL, Walker GM (2003) Management of insecticide resistance in Oriental fruit moth (Grapholita molesta: Lepidoptera: Tortricidae) populations from Ontario. Pest Manag Sci 59:921–927. https://doi.org/10.1002/ps.702

    CAS  Article  PubMed  Google Scholar 

  16. Liu YH, Li BP, Xu ZH (2013) Effect of host instar and temperature on fitness-related traits in the solitary endoparasitoid, Meteorus pulchricornis. Phytoparasitica 41:1–7. https://doi.org/10.1007/s12600-012-0253-1

    Article  Google Scholar 

  17. Mackauer M, Sequeira R, Otto M (1997) Growth and development in parasitoid wasps: adaptation to variable host resources. In: Detner K, Bauer G, Volkl W (eds) Vertical food web interactions: evolutionary patterns and driving forces. Springer, New York, pp 191–203

    Google Scholar 

  18. Mills NJ, Hougardy E, Julier E, Pickel C, Bentley W, Grant J, Wulfert S (2004) Enhancing the biological control of codling moth in walnuts. Walnut Research Reports 2003. Walnut Marketing Board, Sacramento, pp 321–331

  19. Navarro-Roldán MA, Avilla J, Bosch D, Valls J, Gemeno C (2017) Comparative effect of three neurotoxic insecticides with different modes of action on adult males and females of three tortricid moth pests. J Econ Entomol 110:1740–1749. https://doi.org/10.1093/jee/tox113

    CAS  Article  PubMed  Google Scholar 

  20. Oliveira CM, Auad AM, Mendes SM, Frizzas MR (2013) Economic impact of exotic insect pests in Brazilian agriculture. J Appl Entomol 137:1–15. https://doi.org/10.1111/jen.12018

    Article  Google Scholar 

  21. Pastori PL, Arioli CJ, Botton M, Monteiro LB, Stoltman L, Mafra-Neto A (2012) Integrated control of two tortricids (Lepidoptera) pests in apple orchards with sex pheromones and insecticides. Rev Colomb Entomol 38:224–230. https://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-04882012000200010&lng=en&tlng=en

  22. Pettit FL, Wietlisbach DO (1993) Effects of host instar and size on parasitization efficiency and life history parameters of Opius dissitus. Entomol Exp Appl 66:227–236. https://doi.org/10.1111/j.1570-7458.1993.tb00713.x

    Article  Google Scholar 

  23. Quicke DLJ (2015) Idiobionts, koinobionts and other life history traits. The braconid and ichneumonid parasitoid wasps: biology, systematics, evolution and ecology. Wiley, New York, pp 87–106

    Google Scholar 

  24. R Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/. Accessed 26 Sept 2019

  25. Rings RW (1970) Economic aspects of the biology and control of the oriental fruit moth, Grapholitha molesta Busck, in the United States. Ohio J Sci 70:58–61

    Google Scholar 

  26. Sasaki M, Hoshi H, Kawaguchi E (2015) Seasonal prevalence of occurrence of Lytopylus rufipes parasitic on larvae of oriental fruit moth, Grapholita molesta in peach orchards. Ann Rept Plant Prot N Jpn 66:153–156. https://doi.org/10.11455/kitanihon.2015.66_153(in Japanese with English summary)

    Article  Google Scholar 

  27. Sharkey M (1996) The Agathidinae (Hymenoptera: Braconidae) of Japan. Bull Natl Inst Agro-Environ Sci 13:1–100

    Google Scholar 

  28. Trimble RM, Pree DJ, Carter NJ (2001) Integrated control of oriental fruit moth (Lepidoptera: Tortricidae) in peach orchards using insecticide and mating disruption. J Econ Entomol 94:476–485. https://doi.org/10.1603/0022-0493-94.2.476

    CAS  Article  PubMed  Google Scholar 

  29. Uçkan F, Ergin E (2003) Temperature and food source effects on adult longevity of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae). Environ Entomol 32:441–446. https://doi.org/10.1603/0046-225X-32.3.441

    Article  Google Scholar 

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Acknowledgements

We appreciate the kind help of the following researchers: Mr. Masao Kaneko (Nagano Fruit Tree Experiment Station) for the moth colony; Mr. Masatake Sasaki (Fukushima Plant Protection Association) for the wasp colony; Dr. Akihiro Arakawa and Mr. Kouki Yoshida (Fruit Tree Research Centre, Fukushima Prefecture) for preparing immature apples for insect rearing.

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  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Chia-Ming Liu

  2. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Yooichi Kainoh

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  1. Chia-Ming Liu
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  2. Yooichi Kainoh
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Correspondence to Yooichi Kainoh.

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Liu, CM., Kainoh, Y. Laboratory rearing of Lytopylus rufipes (Hymenoptera: Braconidae: Agathidinae), a parasitoid wasp of the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), using apple and a commercially available diet. Appl Entomol Zool 55, 271–276 (2020). https://doi.org/10.1007/s13355-020-00671-0

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Keywords

  • Lytopylus rufipes
  • Grapholita molesta
  • Rearing
  • Transitional diet system