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Identification of novel genes expressed highly and selectively in the corpora allata of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

  • Yuri Homma
  • Kazuei Mita
  • Yuki Nakamura
  • Toshiki Namiki
  • Hiroaki Noda
  • Tetsuro Shinoda
  • Toru TogawaEmail author
Original Research Paper
  • 36 Downloads

Abstract

Juvenile hormone (JH) has crucial roles in insect physiology, including development, reproduction, and polyphenism. JH is synthesized in the corpora allata (CA) from acetyl-CoA through 13 enzymatic steps, which can be divided into the early mevalonate pathway and the late JH branch pathway. Genes for these 13 enzymes have been identified in several insect species, although the three genes for the first three steps in the late pathway are unclear in many insect species. Information about the regulatory mechanism of JH biosynthesis in the CA has been accumulated in previous researches, but the process is not fully understood. In this study, we identified two genes that are expressed highly and selectively in the CA of the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), through the screening using EST library, DNA microarray, and qRT-PCR analyses. These genes showed developmental expression patterns that correlate well with the fluctuation pattern of JH biosynthetic activity in the CA, suggesting important roles in JH biosynthesis or its regulation.

Keywords

Juvenile hormone JH biosynthesis Corpora allata DNA microarray Bombyx mori 

Abbreviations

JH

Juvenile hormone

CA

Corpora allata

CC

Corpora cardiaca

FPP

Farnesyl pyrophosphate

FA

Farnesoic acid

JHAMT

JH acid methyltransferase

Notes

Acknowledgments

This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

Supplementary material

13355_2019_652_MOESM1_ESM.pdf (926 kb)
Supplementary material 1 (PDF 925 kb)
13355_2019_652_MOESM2_ESM.xlsx (39 kb)
Supplementary material 2 (XLSX 38 kb)

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2019

Authors and Affiliations

  1. 1.Department of Biosciences, College of Humanities and SciencesNihon UniversityTokyoJapan
  2. 2.Institute of Agrobiological SciencesNational Agriculture and Food Research OrganizationTsukubaJapan
  3. 3.Faculty of Food and Agricultural SciencesFukushima UniversityFukushimaJapan

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