Applied Entomology and Zoology

, Volume 53, Issue 2, pp 275–279 | Cite as

Application of the LAMP assay for the detection of the Argentine ant, Linepithema humile (Hymenoptera: Formicidae), from captures of pan traps

  • Tatsuya Ide
  • Natsumi Kanzaki
  • Hayato Masuya
  • Kimiko Okabe
Technical Note


We applied the loop-mediated isothermal amplification (LAMP) assay to monitor invasions of Linepithema humile (Mayr), the Argentine ant, a notorious invasive insect worldwide. Species-specific LAMP primers were designed on the basis of the partial sequence of the cytochrome c oxidase subunit I region of Lhumile. The species specificity and sensitivity of these primers were determined in the laboratory and considered adequate for practical use. We also confirmed that the assay successfully detected Lhumile from captures of pan traps, which contained Lhumile and several non-target ant species. The assay detected the target species even when the captures contained only a leg or an antenna. Since the LAMP assay is simple and rapid, this assay will contribute to the early detection and accurate identification of Lhumile.


Invasive alien species Loop-mediated isothermal amplification Molecular identification Monitoring 



We thank Yoshiko Sakamoto and Koichi Goka (National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan) for giving us several samples of Lhumile, information on the bait used, and advice on how to perform field tests. We also thank Asuka Koyama (botanist; Forestry and Forest Products Research Institute) for simplifying our method by giving us feedback after she tested it. This study was supported by the Environment Research and Technology Development Fund (4–1401) from the Ministry of the Environment, Japan.

Supplementary material

13355_2018_546_MOESM1_ESM.pdf (261 kb)
Supplementary material 1 (PDF 260 kb)


  1. Brockerhoff EG, Liebhold AM, Richardson B, Suckling DM (2010) Eradication of invasive forest insects: concepts, methods, costs and benefits. New Zeal J For Sci 40:S117–S135Google Scholar
  2. Cole FR, Medeiros AC, Loope LL, Zuehlke WW (1992) Effects of the Argentine ant on arthropod fauna of Hawaiian high-elevation shrubland. Ecology 73:1313–1322. CrossRefGoogle Scholar
  3. Holway DA (1999) Competitive mechanisms underlying the displacement of native ants by the invasive Argentine ant. Ecology 80:238–251.[0238:CMUTDO]2.0.CO;2Google Scholar
  4. Hulme PE (2009) Trade, transport and trouble: managing invasive species pathways in an era of globalization. J Appl Ecol 46:10–18. CrossRefGoogle Scholar
  5. Human KG, Gordon DM (1996) Exploitation and interference competition between the invasive Argentine ant, Linepithema humile, and native ant species. Oecologia 105:405–412. CrossRefPubMedGoogle Scholar
  6. Ide T, Kanzaki N, Ohmura W, Okabe K (2016a) Molecular identification of an invasive wood-boring insect Lyctus brunneus (Coleoptera: Bostrichidae: Lyctinae) using frass by loop-mediated isothermal amplification and nested PCR assays. J Econ Entomol 109:1410–1414. CrossRefGoogle Scholar
  7. Ide T, Kanzaki N, Ohmura W, Okabe K (2016b) Molecular identification of the western drywood termite (Isoptera: Kalotermitidae) by loop-mediated isothermal amplification of DNA from fecal pellets. J Econ Entomol 109:2234–2237. CrossRefGoogle Scholar
  8. Inoue MN, Goka K (2009) The invasion of alien ants across continents with special reference to Argentine ants and red imported fire ants. Biodiversity 10:67–71. CrossRefGoogle Scholar
  9. Inoue MN, Sunamura E, Suhr EL, Ito F, Tatsuki S, Goka K (2013) Recent range expansion of the Argentine ant in Japan. Divers Distrib 19:29–37. CrossRefGoogle Scholar
  10. Kenis M, Anger-Rozenberg M-A, Roques A, Timms L, Pélé C, Cock MJW, Settele J, Augustin S, Lopez-Vaamonde C (2009) Ecological effects of invasive alien insects. Biol Invasions 11:21–45. CrossRefGoogle Scholar
  11. Liebhold AM, Brockerhoff EG, Garrett LJ, Parke JL, Britton KO (2012) Live plant imports: the major pathway for forest insect and pathogen invasions of the US. Front Ecol Environ 10:135–143. CrossRefGoogle Scholar
  12. Moreau CS, Wray BD, Czekanski-Moir JE, Rubin BER (2013) DNA preservation: a test used preservatives for insects. Invertebr Syst 27:81–86. CrossRefGoogle Scholar
  13. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63. (p. 7)CrossRefPubMedPubMedCentralGoogle Scholar
  14. Notomi T, Mori Y, Tomita N, Kanda H (2015) Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol 53:1–5. CrossRefPubMedGoogle Scholar
  15. Perrings C, Williamson M, Barbier EB, Delfino D, Dalmazzone Dalmazzone, Shogren J, Simmons P, Watkinson A (2002) Biological invasion risks and the public good: an economic perspective. Conserv Ecol 6:1CrossRefGoogle Scholar
  16. Perrings C, Dehnen-Schmutz K, Touza J, Williamson M (2005) How to manage biological invasions under globalization. Trends Ecol Evol 20:212–215. CrossRefPubMedGoogle Scholar
  17. Pimental D, McNair S, Janecka J, Wightman J, Simmonds C, O’Connell C, Wong E, Russel L, Zern J, Aquino T, Tsomondo T (2001) Economic and environmental threats of alien plant, animal, and microbe invasions. Agric Ecosyst Environ 84:1–20. CrossRefGoogle Scholar
  18. Rabaglia R, Duerr D, Acciavatti R, Ragenovich I (2008) Early detection and rapid response for non-native bark and ambrosia beetles. United States Department of Agriculture Forest Service, Forest Health Protection, Washington DCGoogle Scholar
  19. Rassati D, Faccoli M, Toffolo EP, Battisti A, Marini L (2015) Improving the early detection of alien wood-boring beetles in ports and surrounding forests. J Appl Ecol 52:50–58. CrossRefGoogle Scholar
  20. Romero H, Jaffe K (1989) A comparison of methods for sampling ants (Hymenoptera, Formicidae) in Savannas. Biotropica 21:348–352. CrossRefGoogle Scholar
  21. Suarez AV, Holway DA, Case TJ (2001) Patterns of spread in biological invasions dominated by long-distance jump dispersal: insights from Argentine ants. Proc Natl Acad Sci USA 98:1095–1100. CrossRefPubMedPubMedCentralGoogle Scholar
  22. Vilà M, Basnou C, Pyšek P, Josefsson M, Genovesi P, Gollasch S, Nentwig W, Olenin S, Roques A, Roy D et al (2009) How well do we understand the impacts of alien species on ecosystem services? A pan-European, cross-taxa assessment. Front Ecol Environ 8:135–144. CrossRefGoogle Scholar
  23. Vogel V, Pedersen JS, Giraud T, Krieger MJB, Keller L (2010) The worldwide expansion of the Argentine ant. Divers Distrib 16:170–186. CrossRefGoogle Scholar
  24. Wild AL (2004) Taxonomy and distribution of the Argentine ant, Linepithema humile (Hymenoptera: Formicidae). Ann Entomol Soc Am 97:1204–1215.[1204:TADOTA]2.0.CO;2Google Scholar

Copyright information

© The Japanese Society of Applied Entomology and Zoology 2018

Authors and Affiliations

  1. 1.Forestry and Forest Products Research InstituteTsukubaJapan
  2. 2.Department of ZoologyNational Museum of Nature and ScienceTsukubaJapan
  3. 3.Kansai Research CenterForestry and Forest Products Research InstituteKyotoJapan
  4. 4.Tohoku Research CenterForestry and Forest Products Research InstituteMoriokaJapan

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