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Applied Entomology and Zoology

, Volume 53, Issue 2, pp 275–279 | Cite as

Application of the LAMP assay for the detection of the Argentine ant, Linepithema humile (Hymenoptera: Formicidae), from captures of pan traps

  • Tatsuya Ide
  • Natsumi Kanzaki
  • Hayato Masuya
  • Kimiko Okabe
Technical Note

Abstract

We applied the loop-mediated isothermal amplification (LAMP) assay to monitor invasions of Linepithema humile (Mayr), the Argentine ant, a notorious invasive insect worldwide. Species-specific LAMP primers were designed on the basis of the partial sequence of the cytochrome c oxidase subunit I region of Lhumile. The species specificity and sensitivity of these primers were determined in the laboratory and considered adequate for practical use. We also confirmed that the assay successfully detected Lhumile from captures of pan traps, which contained Lhumile and several non-target ant species. The assay detected the target species even when the captures contained only a leg or an antenna. Since the LAMP assay is simple and rapid, this assay will contribute to the early detection and accurate identification of Lhumile.

Keywords

Invasive alien species Loop-mediated isothermal amplification Molecular identification Monitoring 

Notes

Acknowledgements

We thank Yoshiko Sakamoto and Koichi Goka (National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan) for giving us several samples of Lhumile, information on the bait used, and advice on how to perform field tests. We also thank Asuka Koyama (botanist; Forestry and Forest Products Research Institute) for simplifying our method by giving us feedback after she tested it. This study was supported by the Environment Research and Technology Development Fund (4–1401) from the Ministry of the Environment, Japan.

Supplementary material

13355_2018_546_MOESM1_ESM.pdf (261 kb)
Supplementary material 1 (PDF 260 kb)

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2018

Authors and Affiliations

  1. 1.Forestry and Forest Products Research InstituteTsukubaJapan
  2. 2.Department of ZoologyNational Museum of Nature and ScienceTsukubaJapan
  3. 3.Kansai Research CenterForestry and Forest Products Research InstituteKyotoJapan
  4. 4.Tohoku Research CenterForestry and Forest Products Research InstituteMoriokaJapan

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