Applied Entomology and Zoology

, Volume 51, Issue 4, pp 641–651 | Cite as

Sexual difference in antennal sensilla abundance, density and size in Callosobruchus rhodesianus (Coleoptera: Chrysomelidae: Bruchinae)

  • Katsuto Fukuda
  • Aya Yanagawa
  • Midori Tuda
  • Gen Sakurai
  • Satoshi Kamitani
  • Naruto Furuya
Original Research Paper


Sexual difference in antennal sensilla size and per area density has rarely been studied in insects. The bruchine seed beetle Callosobruchus rhodesianus (Pic) (Coleoptera: Chrysomelidae: Bruchinae) is distributed over Central and Southern Africa and is a pest of stored legume seeds, especially cowpeas Vigna unguiculata. Here, we study the type, abundance, morphology and per area density of antennal sensilla on each antennal segment and their sexual differences in C. rhodesianus. The antennae of normal individuals consist of the scape, pedicel and nine flagellomeres as in other congeneric species. Sizes of most antennomeres are larger in males than in females. We observed the following eight different types of antennal sensilla: sensilla trichodea types 1 and 2 (ST1, ST2), sensillacavitae (SCa), sensilla chaetica (SC), sensilla basiconica types 1, 2 and 3 (SB1, SB2, SB3) and Böhm bristles (BB). The SCa is found on all antennomeres unlike in congeners (C. chinensis and C. maculatus). The ST1, the most numerous and longest antennal sensilla type, is denser and thinner in females than in males but more abundant in males than in females. Since the ST1 is considered to serve as a chemoreceptor, the sexual difference in the ST1 may benefit males in searching for mates.


Antennal sensilla Bean beetle Bruchidae Callosobruchus rhodesianus Sensilla per area density Sensory hair thickness 


  1. Adhikary P, Mukherjee A, Barik A (2014) Role of surface wax alkanes from Lathyrus sativus L. seeds for attraction of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). J Stored Prod Res 59:113–119CrossRefGoogle Scholar
  2. Appleby JH, Credland PF (2001) Bionomics and polymorphism in Callosobruchus subinnotatus (Coleoptera: Bruchidae). Bull Entomol Res 91:235–245CrossRefPubMedGoogle Scholar
  3. Awad AA, Ali NA, Mohamed HO (2014) Ultrastructure of the antennal sensillae of male and female peach fruit fly, Bactrocera zonata. J Insect Sci 14:45CrossRefPubMedPubMedCentralGoogle Scholar
  4. Awad AA, Mohamed HO, Ali NA (2015) Differences in antennal sensillae of male and female peach fruit flies in relation to hosts. J Insect Sci 15:1–10CrossRefGoogle Scholar
  5. Clarke KU (1973) The biology of the arthropoda. Elsevier, New YorkGoogle Scholar
  6. Cork A (1991) Identification of a component of the female sex pheromone of Callosobruchus analis (Coleoptera: Bruchidae). Tetrahedron Lett 32:129–132CrossRefGoogle Scholar
  7. Delobel A (2012) Bruchinae (Coleoptera: Chrysomelidae) from Socotra Island. Acta Entomol Mus Natl Pragae 52:373–380Google Scholar
  8. Giga DP, Smith RH (1983) Comparative life history studies of four Callosobruchus species infesting cowpeas with special reference to Callosobruchus rhodesianus (Pic) (Coleoptera: Bruchidae). J Stored Prod Res 19:189–198CrossRefGoogle Scholar
  9. Giga DP, Smith RH (1985) Oviposition markers in Callosobruchus maculatus (F.) and C. rhodesianus (Pic) (Coleoptera: Bruchidae): asymmetry of interspecific responses. Agric Ecosyst Environ 12:229–233CrossRefGoogle Scholar
  10. Giga DP, Smith RH (1987) Egg production and development of Callosobruchus rhodesianus (Pic) and Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) on several commodities at two different temperatures. J Stored Prod Res 23:9–15CrossRefGoogle Scholar
  11. Giga DP, Kadzere I, Canhao J (1993) Bionomics of four strains of Callosobruchus rhodesianus (Pic) (Coleoptela: Bruchidae) infesting different food legumes. J Stored Prod Res 29:19–26CrossRefGoogle Scholar
  12. Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70Google Scholar
  13. Hu F, Zhang GH, Wang JJ (2009) Scanning electron microscopy studies of antennal sensilla of bruchid beetles, Callosobruchus chinensis (L.) and Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Micron 40:320–326CrossRefPubMedGoogle Scholar
  14. Mbata GN, Chinta S, Ramaswamy SB (1997) Morphology of sensory structure on the antennae, maxillary and labial palps, and ovipositor of adult Callosobruchus maculatus and C. subinnotatus. Ann Entomol Soc Am 90:462–469CrossRefGoogle Scholar
  15. Messina FJ, Barmore JL, Renwick JAA (1987) Oviposition deterrent from eggs of Callosobruchus maculatus: spacing mechanism or artifact? J Chem Ecol 13:219–226CrossRefPubMedGoogle Scholar
  16. Nakamura H (1969) Comparative studies of the mating behavior of two species of Callosobruchus (Coleoptera: Bruchidae). Jpn J Ecol 19:20–26Google Scholar
  17. Nojima S, Shimomura K, Honda H, Yamamoto I, Ohsawa K (2007) Contact sex pheromone components of the cowpea weevil, Callosobruchus maculatus. J Chem Ecol 33:923–933CrossRefPubMedGoogle Scholar
  18. Parr MJ, Tran BMD, Simmonds MSJ, Kite GC, Credland PF (1998) Influence of some fatty acids on oviposition by the bruchid beetle, Callosobruchus maculatus. J Chem Ecol 24:1577–1593CrossRefGoogle Scholar
  19. Phillips TW, Phillips JK, Webster FX, Tang R, Burkholder WE (1996) Identification of sex pheromone from cowpea weevil, Callosobruchus maculatus, and related studies with C. analis. J Chem Ecol 22:2233–2249CrossRefPubMedGoogle Scholar
  20. Rup PJ (1988) Antenna and antennal sensilla dimorphism in Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). J Stored Prod Res 24:83–86CrossRefGoogle Scholar
  21. Sakai A, Honda H, Oshima K, Yamamoto I (1986) Oviposition marking pheromone of two bean weevils, Callosobruchus chinensis and Callosobruchus maculatus. J Pestic Sci 11:163–168CrossRefGoogle Scholar
  22. Shimomura K (2007) Homofarnesals: female sex attractant pheromone components of the southern cowpea weevil, Callosobruchus chinensis. J Chem Ecol 34:467–477CrossRefGoogle Scholar
  23. Shimomura K, Koshino H, Yajima A, Matsumoto N, Kagohara Y, Kamada K, Yajima S (2010a) 2, 3-dihydrohomofarnesal: female sex attractant pheromone component of Callosobruchus rhodesianus (Pic). J Chem Ecol 36:824–833CrossRefPubMedGoogle Scholar
  24. Shimomura K, Koshino H, Yajima A, Matsumoto N, Yajima S, Ohsawa K (2010b) A new sesquiterpenoid produced by female Callosobruchus rhodesianus (Pic): a possible component of the sex attractant pheromone. Tetrahedron Lett 51:6860–6862CrossRefGoogle Scholar
  25. Shimomura K, Mimura T, Ishikawa S, Yajima S, Ohsawa K (2010c) Variation in mate recognition specificities among four Callosobruchus seed beetles. Entomol Exp Appl 135:315–322CrossRefGoogle Scholar
  26. Shimomura K, Akasaka K, Yajima A, Mimura T, Yajima S, Ohsawa K (2010d) Contact sex pheromone components of the seed beetle, Callosobruchus analis (F.). J Chem Ecol 36:955–965CrossRefPubMedGoogle Scholar
  27. Shimomura K, Matsui S, Ohsawa K, Yajima S (2016) Saltational evolution of contact sex pheromone compounds of Callosobruchus rhodesianus (Pic). Chemoecology 26:15–23CrossRefGoogle Scholar
  28. Southgate BJ (1979) Biology of the Bruchidae. Annu Rev Entomol 24:449–473CrossRefGoogle Scholar
  29. Tanaka K, Ohsawa K, Honda H, Yamamoto I (1981) Copulation release pheromone, erectin, from the azuki bean weevil (Callosobruchus chinensis L.). J Pestic Sci 6:75–82CrossRefGoogle Scholar
  30. Tanaka K, Ohsawa K, Honda H, Yamamoto I (1982) Synthesis of erectin, a copulation release pheromone of the azuki bean weevil, Callosobruchus chinensis L. J Pestic Sci 7:535–537CrossRefGoogle Scholar
  31. Tuda M (2007) Applied evolutionary ecology of insects of the subfamily Bruchinae (Coleoptera: Chrysomelidae). Appl Entomol Zool 42:337–346CrossRefGoogle Scholar
  32. Tuda M, Chou LY, Niyomdham C, Buranapanichipan S, Tateishi Y (2005) Ecological factors associated with pest status in Callosobruchus (Coleoptera: Bruchidae): high host specificity of non-pests to Cajaninae (Fabaceae). J Stored Prod Res 41:31–45CrossRefGoogle Scholar
  33. Tuda M, Rönn J, Buranapanichpan S, Wasano N, Arnqvist G (2006) Evolutionary diversification of the bean beetle genus Callosobruchus (Coleoptera: Bruchidae): traits associated with stored-product pest status. Mol Ecol 15:3541–3551CrossRefPubMedGoogle Scholar
  34. Watanabe N, Sugimoto S (1988) Geographic variation in male antenna of the azuki bean weevil, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae). Appl Entomol Zool 23:282–290Google Scholar
  35. Zacharuk RY (1985) Antennae and sensilla. In: Kerkut GA, Gilbert LI (eds) Comparative insect physiology, biochemistry and pharmacology, vol 6. Pergamon Press, Oxford, pp 1–69Google Scholar

Copyright information

© The Japanese Society of Applied Entomology and Zoology 2016

Authors and Affiliations

  • Katsuto Fukuda
    • 1
  • Aya Yanagawa
    • 2
  • Midori Tuda
    • 3
    • 4
  • Gen Sakurai
    • 3
    • 5
  • Satoshi Kamitani
    • 6
  • Naruto Furuya
    • 7
  1. 1.Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  2. 2.Research Institute for Sustainable HumanosphereKyoto UniversityUjiJapan
  3. 3.Institute of Biological Control, Faculty of AgricultureKyushu UniversityFukuokaJapan
  4. 4.Laboratory of Insect Natural Enemies, Department of Bioresource Sciences, Faculty of AgricultureKyushu UniversityFukuokaJapan
  5. 5.National Agriculture and Food Research OrganizationInstitute for Agro-Environmental SciencesTsukubaJapan
  6. 6.Entomological Laboratory, Faculty of AgricultureKyushu UniversityFukuokaJapan
  7. 7.Laboratory of Plant Pathology, Faculty of AgricultureKyushu UniversityFukuokaJapan

Personalised recommendations