Sex-specific elicitor from Adoxophyes honmai (Lepidoptera: Tortricidae) induces tea leaf to arrest the egg–larval parasitoid Ascogaster reticulata (Hymenoptera: Braconidae)

Applied Entomology and Zoology volume 51, pages353–362(2016)Cite this article

Abstract

Ascogaster reticulata Watanabe (Hymenoptera: Braconidae) is an egg–larval endoparasitoid wasp of several tortricid species, including Adoxophyes honmai Yasuda (Lepidoptera: Tortricidae). Little information is available on how this parasitoid can find A. honmai eggs using cues produced by tea plants in tritrophic interactions. Therefore, we focused on parasitoid responses to egg-laden tea leaves to determine the source of elicitors of these responses. In two-choice contact bioassays, female wasps spent longer on egg-laden leaves or leaves treated with host female reproductive system homogenates than on intact or untreated leaves at 24 or 48 h after initiating treatment. When tea leaves were treated with diluted host mated-female reproductive system homogenates, wasp residence time was reduced compared with the residence time spent on tea leaves treated with the original concentration of homogenates (reproductive system + 10 μl Ringer’s solution). Moreover, the leaves treated with virgin female reproductive system homogenates also arrested wasps. We conclude that egg deposition by A. honmai induces changes in tea leaves that affect parasitoid behavior. The elicitor of these changes likely occurs in the reproductive system of female moths.

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Acknowledgments

We thank Prof. DeMar Taylor for his careful and critical reading of our manuscript. We also thank Prof. Hiroshi Honda, Assist. Prof. Shigeru Matsuyama, and Assoc. Prof. Seiichi Furukawa for their helpful comments, advice, and corrections of an early version of this manuscript. We are grateful to Yoshiaki Tokushima for advice on statistics. This work was partially supported by a Grant-in-Aid for Scientific Research (no. 23658269) from the Japan Society for the Promotion of Science (JSPS).

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Affiliations

  1. Laboratory of Applied Entomology and Zoology, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Narisara Piyasaengthong

  2. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Natsuko Kinoshita & Yooichi Kainoh

  3. Sugadaira Montane Research Center, University of Tsukuba, Sugadaira Kogen 1278-294, Ueda, Nagano, 386-2204, Japan

    Yukie Sato

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  1. Narisara Piyasaengthong
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  2. Natsuko Kinoshita
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  4. Yooichi Kainoh
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Correspondence to Yooichi Kainoh.

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Piyasaengthong, N., Kinoshita, N., Sato, Y. et al. Sex-specific elicitor from Adoxophyes honmai (Lepidoptera: Tortricidae) induces tea leaf to arrest the egg–larval parasitoid Ascogaster reticulata (Hymenoptera: Braconidae). Appl Entomol Zool 51, 353–362 (2016). https://doi.org/10.1007/s13355-016-0407-6

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Keywords

  • Adoxophyes honmai
  • Ascogaster reticulata
  • Tritrophic interaction
  • Egg-deposition-induced plant defenses
  • Elicitor