Applied Entomology and Zoology

, Volume 51, Issue 1, pp 155–160 | Cite as

A new method for monitoring the susceptibility of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), to pymetrozine by combining topical application and measurement of offspring number

  • Katsuhiko Tsujimoto
  • Shinji Sugii
  • Sachiyo Sanada-Morimura
  • Masaya Matsumura
Technical Note


Pymetrozine is a promising chemical agent for controlling the brown planthopper, Nilaparvata lugens (Stål), after resistance to some neonicotinoid insecticides developed nearly a decade ago. Susceptibility tests by assessing mortality resulting from insecticidal activity are not available for pymetrozine because its key biological action suppresses the reproductive performance of treated N. lugens females. In this study, a new simple method for monitoring the susceptibility of N. lugens to pymetrozine was developed by combining topical application and assessment of the number of offspring using young rice seedlings in a test tube. The ED50 values of the Osaka strain (collected in 1971) and Kumamoto strain (collected in 2011) of N. lugens against pymetrozine were 0.036 and 0.091 μg/g, respectively. In the future, this method will be used to monitor the susceptibility of N. lugens to pymetrozine.


Insecticide susceptibility Nilaparvata lugens Topical application Pymetrozine ED50 



This study was supported in part by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, PRM-3003 to MM).


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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2015

Authors and Affiliations

  • Katsuhiko Tsujimoto
    • 1
  • Shinji Sugii
    • 2
  • Sachiyo Sanada-Morimura
    • 3
  • Masaya Matsumura
    • 3
  1. 1.Syngenta Japan KK, Central Research StationUshikuJapan
  2. 2.Syngenta Japan KKTokyoJapan
  3. 3.NARO Kyushu Okinawa Agriculture Research CenterKoshiJapan

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