Applied Entomology and Zoology

, Volume 49, Issue 1, pp 89–95 | Cite as

Identification of odorant-binding protein genes expressed in the antennae and the legs of the onion fly, Delia antiqua (Diptera: Anthomyiidae)

  • Shinya Ohta
  • Yousuke Seto
  • Koichiro Tamura
  • Yukio Ishikawa
  • Takashi MatsuoEmail author
Original Research Paper


The onion fly, Delia antiqua (Meigen), is a pest specialized to the onion, Allium cepa L., and some other Allium plants. Host odorants play an important role in the attraction of D. antiqua adults and stimulation of oviposition in females. Odorant-binding proteins (OBPs) may serve as a first step in the perception of these chemical cues. In this study, to identify all OBP genes expressed in the chemosensory tissues in D. antiqua, RNA-seq analysis was carried out. In addition to the seven OBP genes previously identified, we found eight novel OBPs. Comparisons with Drosophila melanogaster Meigen OBP genes revealed that these 15 D. antiqua OBPs cover the structural variety observed in D. melanogaster OBPs, including Plus C and Minus C OBPs. These results suggest that a relatively large repertoire of chemosensory genes is maintained even in a specialist feeder.


RNA-seq Oligophagous insect Evolution of chemosensory genes 



This work was supported by JSPS KAKENHI Grant Number 24380030 to TM.


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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2013

Authors and Affiliations

  • Shinya Ohta
    • 1
  • Yousuke Seto
    • 2
  • Koichiro Tamura
    • 2
    • 3
  • Yukio Ishikawa
    • 1
  • Takashi Matsuo
    • 1
    Email author
  1. 1.Department of Agricultural and Environmental BiologyThe University of TokyoTokyoJapan
  2. 2.Department of Biological SciencesTokyo Metropolitan UniversityTokyoJapan
  3. 3.Research Center for Genomics and BioinformaticsTokyo Metropolitan UniversityTokyoJapan

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