Applied Entomology and Zoology

, Volume 47, Issue 1, pp 1–8 | Cite as

Gut symbiotic bacteria in the cabbage bugs Eurydema rugosa and Eurydema dominulus (Heteroptera: Pentatomidae)

  • Yoshitomo Kikuchi
  • Takahiro Hosokawa
  • Naruo Nikoh
  • Takema Fukatsu
Original Research Paper

Abstract

The cabbage bugs Eurydema rugosa Motschulsky and Eurydema dominulus (Scopoli) (Heteroptera: Pentatomidae: Strachiini) possess a number of crypts in a posterior region of the midgut, which are filled with bacterial symbiont cells. Here we characterized the gut symbionts of Eurydema stinkbugs using molecular phylogenetic and histological techniques. Specific gammaproteobacteria were consistently identified from the posterior midgut of E. rugosa representing nine populations and E. dominulus representing six populations, respectively. The bacterial 16S rRNA gene sequences were identical within the species but slightly different (98.2% sequence identity) between the species. Molecular phylogenetic analysis revealed that the Eurydema symbionts formed a well-defined monophyletic group in the Gammaproteobacteria. The symbionts were phylogenetically distinct from the gut symbionts of the stinkbug families Acanthosomatidae, Plataspidae, Parastrachiidae, Scutelleridae, and other pentatomid species, suggesting multiple evolutionary origins of the gut symbiotic bacteria among diverse stinkbugs. In situ hybridization confirmed that the symbiont is located in the cavity of the midgut crypts. Aposymbiotic insects of E. rugosa, which were produced by egg surface sterilization, were viable but suffered retarded growth, reduced body weight, and abnormal body color, suggesting the biological importance of the symbiont for the host.

Keywords

Eurydema rugosa Eurydema dominulus Symbiotic bacteria Midgut crypts Gammaproteobacteria 

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2011

Authors and Affiliations

  • Yoshitomo Kikuchi
    • 1
    • 2
  • Takahiro Hosokawa
    • 3
  • Naruo Nikoh
    • 4
  • Takema Fukatsu
    • 3
  1. 1.National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido CenterSapporoJapan
  2. 2.Graduate School of AgricultureHokkaido UniversitySapporoJapan
  3. 3.National Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan
  4. 4.Department of Liberal ArtsThe Open University of JapanChibaJapan

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