A mitochondrial carrier gene, CG32103, is highly expressed in the corpora allata in the fruit fly Drosophila melanogaster (Diptera: Drosophilidae)
- 215 Downloads
Here we describe a novel gene that is highly expressed in the corpora allata, an endocrine organ responsible for synthesizing juvenile hormones (JHs), in the fruit fly, Drosophila melanogaster Meigen. We isolated an enhancer-trap line in which the transgene was inserted at the locus CG32103, which encodes a mitochondrial carrier family protein with calcium-binding motifs. RNA in situ hybridization revealed that CG32103 is predominantly expressed in the corpora allata in D. melanogaster larvae. Putative orthologs of CG32103 are conserved in many insect species. Mitochondrial carriers are responsible for transporting metabolites across the inner mitochondrial membrane. Given that both mitochondrial membrane transport and cytoplasmic calcium signaling are important for JH biosynthesis regulation, we speculated that CG32103 represents a new member of the family of JH biosynthesis regulators in insects.
KeywordsJuvenile hormone Corpora allata Mitochondrial carrier Drosophila melanogaster
We greatly thank Takashi Adachi-Yamada and Hiroshi Kataoka for support during the initial stages of this work. We also thank Daniel F. Eberl, Günter Korge, Hiroko Sano, the Drosophila Genetic Resource Center at Kyoto Institute of Technology and the Vienna Drosophila RNAi Center for stocks and reagents; Yuko Shimada-Niwa and Tetsuro Shinoda for comments on the manuscript; and Katsuo Furukubo-Tokunaga for allowing us to use his equipment. This work was supported in part by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.
- Carrisi C, Madeo M, Morciano P, Dolce V, Cenci G, Cappello AR, Mazzeo G, Iacopetta D, Capobianco L (2008) Identification of the Drosophila melanogaster mitochondrial citrate carrier: bacterial expression, reconstitution, functional characterization and developmental distribution. J Biochem 144:389–392PubMedCrossRefGoogle Scholar
- Graveley BR, Brooks AN, Carlson JW, Duff MO, Landolin JM, Yang L, Artieri CG, van Baren MJ, Boley N, Booth BW, Brown JB, Cherbas L, Davis CA, Dobin A, Li R, Lin W, Malone JH, Mattiuzzo NR, Miller D, Sturgill D, Tuch BB, Zaleski C, Zhang D, Blanchette M, Dudoit S, Eads B, Green RE, Hammonds A, Jiang L, Kapranov P, Langton L, Perrimon N, Sandler JE, Wan KH, Willingham A, Zhang Y, Zou Y, Andrews J, Bickel PJ, Brenner SE, Brent MR, Cherbas P, Gingeras TR, Hoskins RA, Kaufman TC, Oliver B, Celniker SE (2011) The developmental transcriptome of Drosophila melanogaster. Nature 471:473–479PubMedCrossRefGoogle Scholar
- Huang AE, Rehm J, Rubin GM (2000) Recovery of DNA sequences flanking P-element insertions: inverse PCR and plasmid rescue. In: Sullivan W, Ashburner M, Hawley RS (eds) Drosophila protocols. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Kinjoh T, Kaneko Y, Itoyama K, Mita K, Hiruma K, Shinoda T (2007) Control of juvenile hormone biosynthesis in Bombyx mori: cloning of the enzymes in the mevalonate pathway and assessment of their developmental expression in the corpora allata. Insect Biochem Mol Biol 37:808–818PubMedCrossRefGoogle Scholar
- Larsen MJ, Burton KJ, Zantello MR, Smith VG, Lowery DL, Kubiak TM (2001) Type A allatostatins from Drosophila melanogaster and Diplotera puncata activate two Drosophila allatostatin receptors, DAR-1 and DAR-2, expressed in CHO cells. Biochem Biophys Res Commun 286:895–901PubMedCrossRefGoogle Scholar
- Liu Y, Sheng Z, Liu H, Wen D, He Q, Wang S, Shao W, Jiang RJ, An S, Sun Y, Bendena WG, Wang J, Gilbert LI, Wilson TG, Song Q, Li S (2009) Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila. Development 136:2015–2025PubMedCrossRefGoogle Scholar
- Nijhout HF (1994) Insect hormones. Princeton University Press, PrincetonGoogle Scholar
- Richard DS, Applebaum SW, Sliter TJ, Baker FC, Schooley DA, Reuter CC, Henrich VC, Gilbert LI (1989) Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster: a putative juvenile hormone in the higher Diptera. Proc Natl Acad Sci USA 86:1421–1425PubMedCrossRefGoogle Scholar
- Stapleton M, Carlson J, Brokstein P, Yu C, Champe M, George R, Guarin H, Kronmiller B, Pacleb J, Park S, Wan K, Rubin GM, Celniker SE (2002) A Drosophila full-length cDNA resource. Genome Biol 3:RESEARCH0080Google Scholar