A mitochondrial carrier gene, CG32103, is highly expressed in the corpora allata in the fruit fly Drosophila melanogaster (Diptera: Drosophilidae)
Here we describe a novel gene that is highly expressed in the corpora allata, an endocrine organ responsible for synthesizing juvenile hormones (JHs), in the fruit fly, Drosophila melanogaster Meigen. We isolated an enhancer-trap line in which the transgene was inserted at the locus CG32103, which encodes a mitochondrial carrier family protein with calcium-binding motifs. RNA in situ hybridization revealed that CG32103 is predominantly expressed in the corpora allata in D. melanogaster larvae. Putative orthologs of CG32103 are conserved in many insect species. Mitochondrial carriers are responsible for transporting metabolites across the inner mitochondrial membrane. Given that both mitochondrial membrane transport and cytoplasmic calcium signaling are important for JH biosynthesis regulation, we speculated that CG32103 represents a new member of the family of JH biosynthesis regulators in insects.
KeywordsJuvenile hormone Corpora allata Mitochondrial carrier Drosophila melanogaster
We greatly thank Takashi Adachi-Yamada and Hiroshi Kataoka for support during the initial stages of this work. We also thank Daniel F. Eberl, Günter Korge, Hiroko Sano, the Drosophila Genetic Resource Center at Kyoto Institute of Technology and the Vienna Drosophila RNAi Center for stocks and reagents; Yuko Shimada-Niwa and Tetsuro Shinoda for comments on the manuscript; and Katsuo Furukubo-Tokunaga for allowing us to use his equipment. This work was supported in part by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.
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