Expressions of the cytochrome P450 monooxygenase gene Cyp4g1 and its homolog in the prothoracic glands of the fruit fly Drosophila melanogaster (Diptera: Drosophilidae) and the silkworm Bombyx mori (Lepidoptera: Bombycidae)

  • Ryusuke Niwa
  • Takashi Sakudoh
  • Takeshi Matsuya
  • Toshiki Namiki
  • Shinji Kasai
  • Takashi Tomita
  • Hiroshi Kataoka
Original Research Paper

Abstract

Here we describe the expression profiles of the cytochrome P450 monooxygenase gene Cyp4g1 in the fruit fly, Drosophila melanogaster Meigen, and its homolog in the silkworm, Bombyx mori L. We identified Cyp4g1 by a microarray analysis to examine the expression levels of 86 predicted D. melanogaster P450 genes in the ring gland that contains the prothoracic gland (PG), an endocrine organ responsible for synthesizing ecdysteroids. B. moriCyp4g25 is a closely related homolog of D. melanogaster Cyp4g1 and is also expressed in the PG. A developmental expression pattern of Cyp4g25 in the PG is positively correlated with a fluctuation in hemolymph ecdysteroid titer in the late stage of the final instar. Moreover, the expression of Cyp4g25 in cultured PGs is significantly induced by the addition of prothoracicotropic hormone (PTTH), a neuropeptide hormone that stimulates the synthesis and release of ecdysone. We propose that Cyp4g1 and Cyp4g25 are the candidates that play a role in regulating PG function and control ecdysteroid production and/or metabolism during insect development.

Keywords

Cytochrome P450 monooxygenase Prothoracic gland Bombyx mori Drosophila melanogaster 

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Copyright information

© The Japanese Society of Applied Entomology and Zoology 2011

Authors and Affiliations

  • Ryusuke Niwa
    • 1
  • Takashi Sakudoh
    • 2
  • Takeshi Matsuya
    • 3
  • Toshiki Namiki
    • 3
  • Shinji Kasai
    • 4
  • Takashi Tomita
    • 4
  • Hiroshi Kataoka
    • 3
  1. 1.Initiative for the Promotion of Young Scientists’ Independent Research, Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Division of Radiological Protection and BiologyNational Institute of Infectious DiseasesTokyoJapan
  3. 3.Department of Integrated Biosciences, Graduate School of Frontier SciencesThe University of TokyoKashiwaJapan
  4. 4.Department of Medical EntomologyNational Institute of Infectious DiseasesTokyoJapan

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