Interaction between CA repeat microsatellites and HIF1α regulated the transcriptional activity of porcine IGF1 promoter

  • Hongwei Geng
  • Linlin Hao
  • Yunyun Cheng
  • Chunli Wang
  • Shan Huang
  • Wenzhen Wei
  • Rui Yang
  • Haoyang Li
  • Songcai LiuEmail author
  • Hao YuEmail author
  • Huayi LuEmail author
Animal Genetics • Original Paper


Insulin-like growth factor 1 (IGF1) is pivotal in the regulation of animal growth. Highly polymorphic CA repeat microsatellites have been identified in the IGF1 promoter region of different breeds of pigs. Previous studies showed that CA repeat microsatellites are associated with circulating IGF1 level. However, the mechanisms by which CA repeat microsatellites regulate IGF1 expression remain unclear. This study aimed to detect the association of CA repeat microsatellites with the transcriptional regulation of porcine IGF1 and the possible mechanisms. Results revealed that the number of CA repeats in porcine IGF1 promoter was 14–18, and a promoter with 14 or 15 CA repeats had a higher transcriptional activity (P < 0.01). Transcription factor hypoxia-inducible factor 1 subunit alpha (HIF1α) was confirmed to bind to the binding site upstream of CA repeat microsatellites. The microsatellites with 14 or 15 CA repeats were more sensitive to changes in the HIF1α expression level (P < 0.01). These results suggested that CA repeat microsatellites and HIF1α affected the transcriptional activity of each other in the regulation of IGF1 expression, thereby implying an interaction between them. Overall, this study provided novel evidence for elucidating the effects of CA repeat microsatellites on the transcriptional regulation of porcine IGF1.


Microsatellite IGF1 HIF1α Promoter Transcription 


Funding information

This work was financially supported by the National Natural Science Foundation of China (31672514) and the Technological Development Plan of Jilin Province (20170101024JC and 20190701070GH).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2019

Authors and Affiliations

  1. 1.College of Animal ScienceJilin UniversityChangchunChina
  2. 2.Five-Star Animal Health Pharmaceutical Factory of Jilin ProvinceJilinChina
  3. 3.The Second Hospital of Jilin UniversityJilinChina

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