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Journal of Applied Genetics

, Volume 59, Issue 1, pp 91–97 | Cite as

Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis

  • Marcin Polonis
  • Takafumi Fujimoto
  • Stefan Dobosz
  • Tomasz Zalewski
  • Konrad OcalewiczEmail author
Animal Genetics • Original Paper

Abstract

Rainbow trout (Oncorhynchus mykiss Walbaum) and sea trout (Salmo trutta Linnaeus, 1758) show large karyotypic differences and their hybrid offspring is not viable due to unstable karyotype and chromosome fragmentation. However, gametes from these two species were used to induce gynogenetic development. Rainbow trout eggs activated by UV-irradiated sea trout sperm were subjected to high hydrostatic pressure (HHP) shock to prevent release of the 2nd polar body (early shock) or to inhibit the first cleavage (late shock) in order to produce diploid meiotic gynogenotes and gynogenetic doubled haploids (DHs), respectively. Cytogenetic analysis proved fish that development was induced by the sea trout spermatozoa were rainbow trout. In turn, molecular examination confirmed homozygosity of the gynogenetic DHs. Presumed appearance of the recessive alleles resulted in lower survival of the gynogenetic DH larvae (~25%) when compared to survival of the heterozygous (meiotic) gynogenotes (c. 50%). Our results proved that genomic incompatibilities between studied trout species result in the hybrid unviability. However, artificial gynogenesis including activation of rainbow trout eggs with UV-irradiated sea trout spermatozoa was successfully induced. As both species are unable to cross, application of the UV-irradiated sea trout spermatozoa to activate rainbow trout development assures only maternal inheritance with no contamination by the residues of the paternal chromosomes.

Keywords

Chromosome fragments Gynogenesis Heterologous sperm Hybrids High hydrostatic pressure UV radiation 

Notes

Acknowledgments

This study was supported in part by the National Science Centre (NCN) Poland project number 2014/15/BNZ9/00510 and by JSPS-PAN under the Japan-Poland Research Cooperative Program in 2015 to 2016FY. We thank Janusz Krom and Rafał Rożyński from the Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki for their technical assistance during the experiment.

Author contributions

KO, TZ and SD were involved in designing and performing the experiments. TZ took care of gynogenetic and control embryos and larvae. MP made molecular analysis. TF, KO, and MP were involved in the chromosome preparation, data analysis, writing, and editing the manuscript.

Compliance with ethical standards

Conflict of interest

None of the authors have any conflict of interest to declare.

Supplementary material

13353_2017_425_Fig2_ESM.gif (1.5 mb)
Fig. 1

Sex genotypes of rainbow trout parental individuals and their gynogenetic offspring examined by PCR co-amplification of sdY and 28S rDNA (positive amplification control). Lane 1 and 21: 100 bp DNA ladder (A&A Biotechnology s.c., Poland), lane 2: parental male, lanes from 3 to 6: parental females, lanes from 7 to 20 and from 22 to 26; gynogenetic rainbow trout, lane 27: negative control. sdY marker was found only in rainbow trout male while the positive control 28S rDNA was amplified in all parental and gynogenetic fish (GIF 1507 kb)

13353_2017_425_MOESM1_ESM.tif (4.9 mb)
High resolution image file (TIFF 4972 kb)

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2018

Authors and Affiliations

  • Marcin Polonis
    • 1
  • Takafumi Fujimoto
    • 2
  • Stefan Dobosz
    • 3
  • Tomasz Zalewski
    • 3
  • Konrad Ocalewicz
    • 1
    Email author
  1. 1.Faculty of Oceanography and Geography, Department of Marine Biology and Ecology, Institute of OceanographyUniversity of GdanskGdyniaPoland
  2. 2.Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan
  3. 3.Department of Salmonid ResearchInland Fisheries Institute in OlsztynŻukowoPoland

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