Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH)
The aim of the present work was to study chromosomal polymorphism within cultivated barley (Hordeum vulgare ssp. vulgare) using three-color fluorescence in situ hybridization (FISH). The physical distribution of the most frequently used, highly repetitive DNA sequences (GAA)7 specific for pericentromeric heterochromatic regions, the ribosomal DNA clone pTa71, specific for the 45S rDNA, and the barley-specific telomere-associated sequence HvT01, was investigated to reveal genetic diversity in metaphase spreads of ten barley genotypes with diverse geographical origin, growth habit and row number. A wild relative of barley, Hordeum chilense was also studied in order to compare the polymorphism between and within Hordeum species. Significant differences in the hybridization patterns of all three DNA probes could be detected between the two related species, but only probes pTa71 and HvT01 showed variation in the intensity and/or position of hybridization sites among genotypes of H. vulgare ssp. vulgare. The extent of polymorphism was less than that earlier reported for molecular markers and was restricted to the long chromosome arms, with differences between the chromosomes. 1H and 3H proved to be the most variable chromosomes and 4H and 6H the most conserved.
KeywordsChromosomal polymorphism Hordeum chilense Hordeum vulgare ssp. vulgare Karyotype Three-color FISH
This study was funded by the the Hungarian National Research Fund (OTKA K 104382) and TÁMOP projects (4.2.2.-B-10/1-2010-0025 and 4.2.2.A-11/1/KONV-2012-0064), and was realized in the frames of TÁMOP 4.2.4. A/2-11-1-2012-0001 ‘National Excellence Program – Elaborating and operating an inland student and researcher personal support system convergence program’. The project was subsidized by the European Union and co-financed by the European Social Fund.
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