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Journal of Applied Genetics

, Volume 54, Issue 3, pp 265–269 | Cite as

Molecular cytogenetic characterisation of Salix viminalis L. using repetitive DNA sequences

  • Anna Viktória Németh
  • Dénes Dudits
  • Márta Molnár-Láng
  • Gabriella Linc
Plant Genetics • Short Communication

Abstract

Salix viminalis L. (2n = 38) is a diploid dicot species belonging to the Salix genus of the Salicaceae family. This short-rotation woody crop is one of the most important renewable bioenergy resources worldwide. In breeding for high biomass productivity, limited knowledge is available on the molecular cytogenetics of willow, which could be combined with genetic linkage mapping. The present paper describes the adaptation of a fluorescence in situ hybridisation (FISH) protocol as a new approach to analyse the genomic constitution of Salix viminalis using the heterologous DNA clones pSc119.2, pTa71, pTa794, pAs1, Afa-family, pAl1, HT100.3, ZCF1 and the GAA microsatellite marker. Three of the nine probes showed unambiguous signals on the metaphase chromosomes. FISH analysis with the pTa71 probe detected one major 18S-5.8S-26S rDNA locus on the short arm of one chromosome pair; however, the pTa794 rDNA site was not visible. One chromosome pair showed a distinct signal around the centromeric region after FISH with the telomere-specific DNA clone HT100.3. Two chromosome pairs were found to have pAs1 FISH signals, which represent a D-genome-specific insert from Aegilops tauschii. Based on the FISH study, a set of chromosomes with characteristic patterns is presented, which could be used to establish the karyotype of willow species.

Keywords

FISH Genome constitution Repetitive DNA clones Salix viminalis 

Notes

Acknowledgements

This study was funded by Kreátor 2005 Ltd. (Budapest), TÁMOP-4.2.2/B-10/1-2012-0025) and a János Bolyai Research Scholarship from HAS to G.L. The authors thank Barbara Harasztos for revising the manuscript linguistically.

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2013

Authors and Affiliations

  • Anna Viktória Németh
    • 1
  • Dénes Dudits
    • 1
  • Márta Molnár-Láng
    • 2
  • Gabriella Linc
    • 2
  1. 1.Institute of Plant Biology, Biological Research CentreHungarian Academy of SciencesSzegedHungary
  2. 2.Agricultural Institute, Centre for Agricultural ResearchHungarian Academy of SciencesMartonvásárHungary

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