Journal of Applied Genetics

, Volume 54, Issue 1, pp 113–118 | Cite as

Candidate gene expression affects intramuscular fat content and fatty acid composition in pigs

  • Wei Wang
  • Wenda Xue
  • Bangquan Jin
  • Xixia Zhang
  • Fei Ma
  • Xiaofeng Xu
Animal Genetics • Original Paper

Abstract

The objective of this study was to correlate the expression pattern of candidate genes with the intramuscular fat (IMF) content and fatty acid composition of the Longissimus dorsi muscle of Duroc × Shanzhu commercial crossbred pigs. Animals of both sexes were slaughtered at a body weight of about 90 kg. The IMF content and fatty acid composition of the Longissimus dorsi muscle were measured and correlated with candidate genes mRNA expression (AdPLA, ADRB3, LEPR, MC4R, PPARγ, PPARα, LPL, PEPCK, and SCD). Females presented higher IMF content (p < 0.05) than males. The total saturated fatty acid (SFA) in males was greater (p < 0.01), whereas the total monounsaturated fatty acid (MUFA) (p < 0.01) and polyunsaturated fatty acid (PUFA) (p < 0.05) were lower than in females. The expressions of AdPLA, MC4R, PEPCK, and SCD correlated with the IMF content (p < 0.05). AdPLA showed a positive association with MUFA and a negative association with SFA (p < 0.05). LEPR and MC4R were both positively and significantly associated with C18:3 and C20:0 (p < 0.05). PPARα and PPARγ were negatively correlated with SFA, and PPARγ was positively associated with MUFA (p < 0.05). LPL was positively associated with MUFA and negatively associated with SFA (p < 0.05). PEPCK was negatively correlated with PUFA (p < 0.05). SCD was positively associated with MUFA (p < 0.05). The revealed correlations may confirm that these candidate genes are important for fat deposition and fatty acid composition in pigs, and the evaluation and use of these genes may be useful for improving porcine meat quality.

Keywords

Fatty acid IMF Longissimus muscle Pig Real-time quantitative PCR 

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2012

Authors and Affiliations

  • Wei Wang
    • 1
  • Wenda Xue
    • 1
  • Bangquan Jin
    • 2
  • Xixia Zhang
    • 3
  • Fei Ma
    • 1
  • Xiaofeng Xu
    • 1
  1. 1.State Key Discipline of Zoology, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life SciencesNanjing Normal UniversityNanjingChina
  2. 2.Department of Food Science and NutritionNanjing Normal UniversityNanjingChina
  3. 3.Institute of Husbandry and Poultry ResearchNanjingChina

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