Journal of Applied Genetics

, Volume 53, Issue 4, pp 443–448 | Cite as

Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens

  • Shi-Jie Lv
  • Ling Su
  • Hong Li
  • Rui-Li Han
  • Gui-Rong Sun
  • Xiang-Tao Kang
Animal Genetics • Original Paper

Abstract

Interleukin-15 (IL-15) is a cytokine that has been proposed to modulate skeletal muscle and adipose tissue mass. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken IL-15 gene. Two single nucleotide polymorphisms (SNPs) (g.31224G>A and g.31266T>G) were identified in exon 5 of the IL-15 gene by means of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. Associations between the two SNPs and chicken fatness and muscle fiber traits were determined using linkage disequilibrium, haplotype construction, and association analysis. Both of the SNPs were associated with abdominal fat weight, leg muscle fiber diameter, and leg muscle fiber density (p < 0.05). Haplotypes of the two linked SNPs were associated with abdominal fat weight, fat thickness under the skin, and leg muscle fiber diameter (p < 0.05). The results suggested that the IL-15 gene might be associated with the causative mutation or the quantitative trait locus (QTL) controlling the fatness traits and muscle fiber traits in chickens.

Keywords

Chicken IL-15 gene Single nucleotide polymorphism Haplotype Fatness and muscle fiber traits 

Notes

Acknowledgments

The project was supported by the National Natural Science Foundation of China (nos. 31072023 and 31101707) and the Earmarked Fund for Modern Agro-Industry Technology Research System (no. CARS-41-K04).

References

  1. Alvarez B, Carbó N, López-Soriano J, Drivdahl RH, Busquets S, López-Soriano FJ, Argilés JM, Quinn LBS (2002) Effects of interleukin-15 (IL-15) on adipose tissue mass in rodent obesity models: evidence for direct IL-15 action on adipose tissue. Biochim Biophys Acta 1570:33–37PubMedCrossRefGoogle Scholar
  2. Argilés JM, López-Soriano J, Almendro V, Busquets S, López-Soriano FJ (2005) Cross-talk between skeletal muscle and adipose tissue: a link with obesity? Med Res Rev 25:49–65PubMedCrossRefGoogle Scholar
  3. Barra NG, Reid S, MacKenzie R, Werstuck G, Trigatti BL, Richards C, Holloway AC, Ashkar AA (2010) Interleukin-15 contributes to the regulation of murine adipose tissue and human adipocytes. Obesity (Silver Spring) 18:1601–1607CrossRefGoogle Scholar
  4. Carbó N, López-Soriano J, Costelli P, Alvarez B, Busquets S, Baccino FM, Quinn LBS, López-Soriano FJ, Argilés JM (2001) Interleukin-15 mediates reciprocal regulation of adipose and muscle mass: a potential role in body weight control. Biochim Biophys Acta 1526:17–24PubMedCrossRefGoogle Scholar
  5. Carson WE, Giri JG, Lindemann MJ, Linett ML, Ahdieh M, Paxton R, Anderson D, Eisenmann J, Grabstein K, Caligiuri MA (1994) Interleukin (IL) 15 is a novel cytokine that activates human natural killer cells via components of the IL-2 receptor. J Exp Med 180:1395–1403PubMedCrossRefGoogle Scholar
  6. Fehniger TA, Caligiuri MA (2001) Interleukin 15: biology and relevance to human disease. Blood 97:14–32PubMedCrossRefGoogle Scholar
  7. Furmanczyk PS, Quinn LBS (2003) Interleukin-15 increases myosin accretion in human skeletal myogenic cultures. Cell Biol Int 27:845–851PubMedCrossRefGoogle Scholar
  8. Giri JG, Kumaki S, Ahdieh M, Friend DJ, Loomis A, Shanebeck K, DuBose R, Cosman D, Park LS, Anderson DM (1995) Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor. EMBO J 14:3654–3663PubMedGoogle Scholar
  9. Grabstein KH, Eisenman J, Shanebeck K, Rauch C, Srinivasan S, Fung V, Beers C, Richardson J, Schoenborn MA, Ahdieh M, Johnson L, Alderson MR, Watson JD, Anderson DM, Giri JG (1994) Cloning of a T cell growth factor that interacts with the beta chain of the interleukin-2 receptor. Science 264:965–968PubMedCrossRefGoogle Scholar
  10. Han RL, Lan XY, Zhang LZ, Ren G, Jing YJ, Li MJ, Zhang B, Zhao M, Guo YK, Kang XT, Chen H (2010) A novel single-nucleotide polymorphism of the visfatin gene and its associations with performance traits in the chicken. J Appl Genet 51:59–65PubMedCrossRefGoogle Scholar
  11. Han RL, Li ZJ, Li MJ, Li JQ, Lan XY, Sun GR, Kang XT, Chen H (2011) Novel 9-bp indel in visfatin gene and its associations with chicken growth. Br Poult Sci 52:52–57PubMedCrossRefGoogle Scholar
  12. Jiang RR, Zhao GP, Chen JL, Zheng MQ, Zhao JP, Li P, Hu J, Wen J (2011) Effect of dietary supplemental nicotinic acid on growth performance, carcass characteristics and meat quality in three genotypes of chicken. J Anim Physiol Anim Nutr (Berl) 95:137–145CrossRefGoogle Scholar
  13. Nielsen AR, Mounier R, Plomgaard P, Mortensen OH, Penkowa M, Speerschneider T, Pilegaard H, Pedersen BK (2007) Expression of interleukin-15 in human skeletal muscle effect of exercise and muscle fibre type composition. J Physiol 584:305–312PubMedCrossRefGoogle Scholar
  14. Nielsen AR, Hojman P, Erikstrup C, Fischer CP, Plomgaard P, Mounier R, Mortensen OH, Broholm C, Taudorf S, Krogh-Madsen R, Lindegaard B, Petersen AM, Gehl J, Pedersen BK (2008) Association between interleukin-15 and obesity: interleukin-15 as a potential regulator of fat mass. J Clin Endocrinol Metab 93:4486–4493PubMedCrossRefGoogle Scholar
  15. Pistilli EE, Siu PM, Alway SE (2007) Interleukin-15 responses to aging and unloading-induced skeletal muscle atrophy. Am J Physiol Cell Physiol 292:C1298–C1304PubMedCrossRefGoogle Scholar
  16. Pistilli EE, Devaney JM, Gordish-Dressman H, Bradbury MK, Seip RL, Thompson PD, Angelopoulos TJ, Clarkson PM, Moyna NM, Pescatello LS, Visich PS, Zoeller RF, Gordon PM, Hoffman EP (2008) Interleukin-15 and interleukin-15Rα SNPs and associations with muscle, bone, and predictors of the metabolic syndrome. Cytokine 43:45–53PubMedCrossRefGoogle Scholar
  17. Pistilli EE, Bogdanovich S, Garton F, Yang N, Gulbin JP, Conner JD, Anderson BG, Quinn LBS, North K, Ahima RS, Khurana TS (2011) Loss of IL-15 receptor α alters the endurance, fatigability, and metabolic characteristics of mouse fast skeletal muscles. J Clin Invest 121:3120–3132PubMedCrossRefGoogle Scholar
  18. Quinn LS (2008) Interleukin-15: a muscle-derived cytokine regulating fat-to-lean body composition. J Anim Sci 86:E75–E83PubMedCrossRefGoogle Scholar
  19. Quinn LS, Anderson BG (2011) Interleukin-15, IL-15 receptor-alpha, and obesity: concordance of laboratory animal and human genetic studies. J Obes 2011:1–8; Article ID 456347CrossRefGoogle Scholar
  20. Quinn LS, Haugk KL, Grabstein KH (1995) Interleukin-15: a novel anabolic cytokine for skeletal muscle. Endocrinology 136:3669–3672PubMedCrossRefGoogle Scholar
  21. Quinn LBS, Anderson BG, Drivdahl RH, Alvarez B, Argilés JM (2002) Overexpression of interleukin-15 induces skeletal muscle hypertrophy in vitro: implications for treatment of muscle wasting disorders. Exp Cell Res 280:55–63PubMedCrossRefGoogle Scholar
  22. Quinn LBS, Strait-Bodey L, Anderson BG, Argilés JM, Havel PJ (2005) Interleukin-15 stimulates adiponectin secretion by 3T3-L1 adipocytes: evidence for a skeletal muscle-to-fat signaling pathway. Cell Biol Int 29:449–457PubMedCrossRefGoogle Scholar
  23. Quinn LBS, Anderson BG, Strait-Bodey L, Stroud AM, Argilés JM (2009) Oversecretion of interleukin-15 from skeletal muscle reduces adiposity. Am J Physiol Endocrinol Metab 296:E191–E202PubMedCrossRefGoogle Scholar
  24. Riechman SE, Balasekaran G, Roth SM, Ferrell RE (2004) Association of interleukin-15 protein and interleukin-15 receptor genetic variation with resistance exercise training responses. J Appl Physiol 97:2214–2219PubMedCrossRefGoogle Scholar
  25. Sugiura T, Harigai M, Kawaguchi Y, Takagi K, Fukasawa C, Ohsako-Higami S, Ohta S, Tanaka M, Hara M, Kamatani N (2002) Increased IL-15 production of muscle cells in polymyositis and dermatomyositis. Int Immunol 14:917–924PubMedCrossRefGoogle Scholar
  26. Zhang ZR, Liu YP, Jiang X, Du HR, Zhu Q (2008) Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fibre and carcass traits in quality chicken populations. J Anim Breed Genet 125:258–264PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2012

Authors and Affiliations

  • Shi-Jie Lv
    • 1
  • Ling Su
    • 1
  • Hong Li
    • 1
  • Rui-Li Han
    • 1
  • Gui-Rong Sun
    • 1
  • Xiang-Tao Kang
    • 1
  1. 1.College of Animal Science and Veterinary MedicineHenan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm ResourceZhengzhouPeople’s Republic of China

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